Click image to see more details
Product Info Summary
| SKU: | PB9710 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-IGFBP3 Antibody Picoband®
SKU/Catalog Number
PB9710
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IGFBP3 Antibody Picoband® catalog # PB9710. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IGFBP3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9710)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human IGFBP-3, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9710 is reactive to IGFBP3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
40 kDa
Calculated molecular weight
31674 MW
Background of IGFBP-3
IGFBP3, Insulin-like growth fator-binding protein 3, is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. IGFBP3 is located on chromosome 7. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9710 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: rat heart tissue, rat brain tissue, rat liver tissue, rat PC-12 whole cellhuman U-87MG whole cellmouse kidney tissue, mouse heart tissue, mouse brain tissue,
IHC: Human Intestinal Cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of IGFBP-3 using anti-IGFBP-3 antibody (PB9710).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat liver tissue lysates,
Lane 4: rat PC-12 whole cell lysates.
Lane 5: human U-87MG whole cell lysates.
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse brain tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP-3 antigen affinity purified polyclonal antibody (Catalog # PB9710) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP-3 at approximately 40KD. The expected band size for IGFBP-3 is at 32KD.
Click image to see more details
Figure 2. IHC analysis of IGFBP3 using anti-IGFBP3 antibody (PB9710).
IGFBP3 was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IGFBP3 Antibody (PB9710) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For IGFBP3 (Source: Uniprot.org, NCBI)
Gene Name
IGFBP3
Full Name
Insulin-like growth factor-binding protein 3
Weight
31674 MW
Alternative Names
Insulin-like growth factor-binding protein 3;IBP-3;IGF-binding protein 3;IGFBP-3;IGFBP3;IBP3; IGFBP3 BP-53, IBP3 insulin like growth factor binding protein 3 insulin-like growth factor-binding protein 3|IBP-3|IGF-binding protein 3|IGFBP-3|acid stable subunit of the 140 K IGF complex|binding protein 29|binding protein 53|growth hormone-dependent binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IGFBP3, check out the IGFBP3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IGFBP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IGFBP3 Antibody Picoband® (PB9710)
Hello CJ!
PB9710 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Correlation between insulin‑like growth factor binding protein 3 and metastasis‑associated gene 1 protein in esophageal squamous cell carcinoma
Correlation between insulin%u2011like growth factor binding protein 3 and metastasis%u2011associated gene 1 protein in esophageal squamous cell carcinoma
Pu Xy, Zheng Xg, Zhang Y, Xiao Hj, Xu Zp, Liu Jm, Wang Hp, Wen Am, Zhou Xx, Wu Yl. J Sex Med. 2011 Aug;8(8):2181-90. Doi: 10.1111/J.1743-6109.2011.02318.X. Epub 2011 May 19. Higher Expression Of Mrna And Protein Of Insulin-Like Growth Factor Bindi...
Pu Xy, Wen Am, Liu Jm, Zheng Xg, Xiao Hj, Xu Zp, Wang Hp, Li D, Zhang Rl. J Urol. 2014 Sep;192(3):990-6. Doi: 10.1016/J.Juro.2014.02.042. Epub 2014 Feb 24. Shrna Constructs Targeting Igfbp-3 Alleviate Age Related Erectile Dysfunction In The Rat.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-IGFBP3 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-IGFBP3 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-IGFBP3 Antibody Picoband®
Question
I am interested in to test anti-IGFBP3 antibody PB9710 on rat cerebrospinal fluid for research purposes, then I may be interested in using anti-IGFBP3 antibody PB9710 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
The products we sell, including anti-IGFBP3 antibody PB9710, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-24
Question
Is a blocking peptide available for product anti-IGFBP3 antibody (PB9710)?
A. Johnson
Verified customer
Asked: 2019-10-16
Answer
We do provide the blocking peptide for product anti-IGFBP3 antibody (PB9710). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-16
Question
We are currently using anti-IGFBP3 antibody PB9710 for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
H. Singh
Verified customer
Asked: 2018-06-18
Answer
The anti-IGFBP3 antibody (PB9710) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-18
Question
Does anti-IGFBP3 antibody PB9710 work on feline IHC with kidney spleen?
Verified Customer
Verified customer
Asked: 2017-11-13
Answer
Our lab technicians have not validated anti-IGFBP3 antibody PB9710 on feline. You can run a BLAST between feline and the immunogen sequence of anti-IGFBP3 antibody PB9710 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline kidney spleen in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-13
Question
Would anti-IGFBP3 antibody PB9710 work for WB with cerebrospinal fluid?
A. Baker
Verified customer
Asked: 2017-02-07
Answer
According to the expression profile of cerebrospinal fluid, IGFBP3 is highly expressed in cerebrospinal fluid. So, it is likely that anti-IGFBP3 antibody PB9710 will work for WB with cerebrospinal fluid.
Boster Scientific Support
Answered: 2017-02-07
