Product Info Summary
SKU: | PROTP22692 |
---|---|
Size: | 5ug, 20ug, 1mg |
Origin Species: | Human |
Source: | HEK293 |
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Product info
Product Name
IGFBP-4 Insulin-Like Growth Factor Binding Protein-4 Human
View all IGFBP-4 recombinant proteins
SKU/Catalog Number
PROTP22692
Size
5ug, 20ug, 1mg
Description
IGFBP-4 Human Recombinant amino acids Asp22- Glu258, produced in HEK293 cells and fused with a polyhistidine tag at the C-terminus. IGFBP4 predicted Mw is 27kDa and on SDS-PAGE appears as a 32kDa band under denaturing conditions. IGFBP4 is purified by proprietary chromatographic techniques.
Storage & Handling
Lyophilized IGFBP4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFBP-4 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Cite This Product
IGFBP-4 Insulin-Like Growth Factor Binding Protein-4 Human (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP22692)
Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
IGFBP 4 was lyophilized after extensive dialysis against PBS.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Predicted MW
27.934kDa
Reconstitution
It is recommended to reconstitute the lyophilized IGFBP4 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Amino Acid Sequence
DEAIHCPPCS EEKLARCRPP VGCEELVREP GCGCCATCAL GLGMPCGVYT PRCGSGLRCY PPRGVEKPLH TLMHGQGVCM ELAEIEAIQE SLQPSDKDEG DHPNNSFSPC SAHDRRCLQK HFAKIRDRST SGGKMKVNGA PREDARPVPQ GSCQSELHRA LERLAASQSR THEDLYIIPI PNCDRNGNFH PKQCHPALDG QRGKCWCVDR KTGVKLPGGL EPKGELDCHQ LADSFRE + His Tag
Biological Activity
The ED50 range is 0.01-0.09µg/ml as measured by its ability to inhibit the biological activity of IGFII (20ng/ml) on MCF7 human breast adenocarcinoma cells.
Assay dilution & Images
Reconstitution
It is recommended to reconstitute the lyophilized IGFBP4 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Validation Images & Assay Conditions
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Recombinant protein fun image
Protein Target Info & Infographic
Gene/Protein Information For IGFBP4 (Source: Uniprot.org, NCBI)
Gene Name
IGFBP4
Full Name
Insulin-like growth factor-binding protein 4
Weight
27.934kDa
Alternative Names
Insulin-like growth factor-binding protein 4; IBP-4; IGF-binding protein 4; IGFBP-4; IGFBP4; IBP4; BP-4; HT29-IGFBP IGFBP4 BP-4, HT29-IGFBP, IBP4, IGFBP-4 insulin like growth factor binding protein 4 insulin-like growth factor-binding protein 4|IBP-4|IGF-binding protein 4
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on IGFBP4, check out the IGFBP4 Infographic
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In this infographic, you will see the following information for IGFBP4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For IGFBP-4 Insulin-Like Growth Factor Binding Protein-4 Human (PROTP22692)
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