Product Info Summary
SKU: | PROTP09920 |
---|---|
Size: | 2ug, 10ug, 1mg |
Origin Species: | Mouse |
Source: | Escherichia coli |
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Product info
Product Name
G-CSF Granulocyte-Colony Stimulating Factor Mouse Recombinant Protein
View all G-CSF recombinant proteins
SKU/Catalog Number
PROTP09920
Size
2ug, 10ug, 1mg
Description
Granulocyte Colony Stimulating Factor Mouse Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 178 amino acids and having a molecular mass of approximately 18.9kDa. G-CSF is purified by proprietary chromatographic techniques.
Storage & Handling
Lyophilized Granulocyte Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GCSF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Cite This Product
G-CSF Granulocyte-Colony Stimulating Factor Mouse Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP09920)
Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
G-CSF Lyophilized from 10mM NaCitrate, pH 4.0 and 150mM NaCl.
Purity
Greater than 98.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.
Predicted MW
22.293kDa
Reconstitution
It is recommended to reconstitute the lyophilized Granulocyte Colony Stimulating Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Amino Acid Sequence
VPLVTVSAL PPSLPLPRSF LLKSLEQVRK IQASGSVLLE QLCATYKLCH PEELVLLGHS LGIPKASLSG CSSQALQQTQ CLSQLHSGLC LYQGLLQALS GISPALAPTL DLLQLDVANF ATTIWQQMEN LGVAPTVQPT QSAMPAFTSA FQRRAGGVLA ISYLQGFLET ARLALHHLA
Biological Activity
The ED50 as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05 ng/ml, corresponding to a Specific Activity of 2 x 107IU/mg.
Assay dilution & Images
Reconstitution
It is recommended to reconstitute the lyophilized Granulocyte Colony Stimulating Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Validation Images & Assay Conditions
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Recombinant protein fun image
Protein Target Info & Infographic
Gene/Protein Information For CSF3 (Source: Uniprot.org, NCBI)
Gene Name
CSF3
Full Name
Granulocyte colony-stimulating factor
Weight
22.293kDa
Superfamily
IL-6 superfamily
Alternative Names
CSF3; MGI-1G; GM-CSF beta; Pluripoietin; G-CSF; GCSF CSF3 C17orf33OS, GCSF, CSF3 colony stimulating factor 3 granulocyte colony-stimulating factor|filgrastim|granulocyte-colony stimulating factor|lenograstim|pluripoietin
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on CSF3, check out the CSF3 Infographic
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In this infographic, you will see the following information for CSF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For G-CSF Granulocyte-Colony Stimulating Factor Mouse Recombinant Protein (PROTP09920)
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