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Product Info Summary
| SKU: | EK0361 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse G-CSF / CSF3 ELISA Kit PicoKine®
SKU/Catalog Number
EK0361
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse G-CSF / CSF3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Csf3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 4pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse G-CSF / CSF3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0361)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: V31-A208
Sensitivity
<4 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0361 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0361 is reactive to Csf3 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0361 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of G-CSF
Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of hormone-like glycoprotein that regulates hematopoietic cell proliferation and differentiation, and it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture. G-CSF is also termed colony stimulating factor-3, and a single gene of which codes for a 177 or 180 amino acid mature protein of molecular weight 19,600. Functionally, it specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity. Besides, it also prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. Human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17.3 The standard product used in this kit is recombinant Mouse G-csf, consisting of 179 amino acids with the molecular mass of 19KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0361-CAP | Anti-Mouse Csf3 Pre-coated 96-well strip microplate | 1 |
| EK0361-ST | Mouse Csf3 Standard | 2 vials, 10 ng/tube |
| EK0361-DA | Mouse Csf3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.034 | 0.094 | 0.169 | 0.294 | 0.613 | 1.320 | 1.822 | 1.990 |
Data Example Images
Click image to see more details
Mouse G-CSF PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect G-CSF, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 31.2 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse G-CSF / CSF3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 54 | 247 | 1009 | 49 | 250 | 976 |
| Standard deviation | 4.15 | 12.59 | 58.52 | 3.96 | 16.25 | 70.27 |
| CV (%) | 7.7% | 7.2%% | 5.8% | 8.1% | 6.5% | 7.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of G-CSF in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 54 | 53 | 52 | 59 | 54 | 2.69 | 4.9% |
| Sample 2 | 247 | 297 | 262 | 252 | 264 | 19.52 | 7.3% |
| Sample 3 | 1009 | 966 | 904 | 972 | 962 | 37.7 | 3.9% |
Protein Target Info & Infographic
Gene/Protein Information For Csf3 (Source: Uniprot.Org, NCBI)
Gene Name
Csf3
Full Name
Granulocyte colony-stimulating factor
Weight
22421 MW
Superfamily
IL-6 superfamily
Alternative Names
Granulocyte colony-stimulating factor;G-CSF;Csf3;Csfg; CSF3 C17orf33OS, GCSF, CSF3 colony stimulating factor 3 granulocyte colony-stimulating factor|filgrastim|granulocyte-colony stimulating factor|lenograstim|pluripoietin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Csf3, check out the Csf3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Csf3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse G-CSF / CSF3 ELISA Kit PicoKine® (EK0361)
Hello CJ!
EK0361 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effects of ferulic acid on hematopoietic cell recovery in whole-body gamma irradiated mice
Improvement of icaritin on hematopoietic function in cyclophosphamide-induced myelosuppression mice
HSF1 regulates expression of G-CSF through the binding element for NF-IL6/CCAAT enhancer binding protein beta
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Mouse G-CSF / CSF3 ELISA Kit PicoKine®
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of G-CSF in Mouse cell lysates? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Z. Green
Verified customer
Asked: 2020-11-26
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the G-CSF ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-11-26
Question
Q: Can G-CSF ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-09-19
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-09-19
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
B. Wilson
Verified customer
Asked: 2020-07-27
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-07-27
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
W. Jones
Verified customer
Asked: 2020-05-25
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-05-25
Question
Q: is it okay to use citrate plasma as samples in Mouse G-CSF Picokine® ELISA Kit (Catalog # EK0361)?
W. Collins
Verified customer
Asked: 2019-04-19
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of G-CSF causing degradation of its protein structure. G-CSF may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the G-CSF ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-04-19
Question
Q: if the enzyme conjugated G-CSF antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the G-CSF antigen?
T. Lopez
Verified customer
Asked: 2019-02-27
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-02-27
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis G-CSF using the EK0361 Mouse G-CSF Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-02-22
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2018-02-22
Question
Q: how to thaw whole blood sample for G-CSF ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-02-04
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test G-CSF for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-02-04
