Mouse MIP-1Alpha/CCL3 ELISA Kit PicoKine®

Product Name

Mouse MIP-1Alpha/CCL3 ELISA Kit PicoKine®

View all CCL3 ELISA kits

SKU/Catalog Number

EK0449

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse MIP-1Alpha/CCL3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ccl3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse MIP-1Alpha/CCL3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0449)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: A24-A92

Sensitivity

<2 pg/ml

Assay Range

7.8 pg/ml - 500 pg/ml

Standard Dilution Instructions

See datasheet of EK0449 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0449 is reactive to Ccl3 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0449 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CCL3

Chemokine (C-C motif) ligand 3 (CCL3), so known as Macrophage inflammatory protein-1alpha (MIP-1 alpha), is a protein that in humans is encoded by the CCL3 gene. It is a cytokine which belongs to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This gene is mapped to 17q12. CCL3 plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. It has been found that the activation of CCR5 by CCL3 directly and independently activates a G-protein signaling pathway through GNAI2 and a tyrosine phosphorylation signaling pathway through JAK2.

Kit Components

Catalog Number	Description	Quantity
EK0449-CAP	Anti-Mouse Ccl3 Pre-coated 96-well strip microplate	1
EK0449-ST	Mouse Ccl3 Standard	2 vials, 10 ng/tube
EK0449-DA	Mouse Ccl3 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
O.D.	0.106	0.134	0.166	0.226	0.345	0.529	1.028	1.804

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect CCL3, Dilution ratio of 1:1, concentration in serum and plasma is around 50 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse MIP-1Alpha/CCL3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CCL3 in ELISA kits from four different production batches/lots.

Gene/Protein Information For Ccl3 (Source: Uniprot.Org, NCBI)

Alternative Names

C-C motif chemokine 3;Heparin-binding chemotaxis protein;L2G25B;Macrophage inflammatory protein 1-alpha;MIP-1-alpha;SIS-alpha;Small-inducible cytokine A3;TY-5;Ccl3;Mip1a, Scya3; CCL3 G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, SCYA3 C-C motif chemokine ligand 3 C-C motif chemokine 3|G0/G1 switch regulatory protein 19-1|PAT 464.1|SIS-beta|macrophage inflammatory protein 1-alpha|small inducible cytokine A3 (homologous to mouse Mip-1a)|tonsillar lymphocyte LD78 alpha protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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