GCSF Granulocyte-Colony Stimulating Factor Rat Recombinant Protein

Product Name

GCSF Granulocyte-Colony Stimulating Factor Rat Recombinant Protein

View all G-CSF recombinant proteins

SKU/Catalog Number

PROTP97712

Size

2ug, 10ug, 1mg

Description

GCSF Rat Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 195 amino acids and having a molecular mass of 21.5kDa. The G-CSF is purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized G-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GCSF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Cite This Product

GCSF Granulocyte-Colony Stimulating Factor Rat Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP97712)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2µm filtered solution in 5mM Sodium Citrate, pH 4.0.

Purity

Greater than 97.0% as determined by (a)Analysis by RP-HPLC and (b)Analysis by SDS-PAGE.

Predicted MW

22.293kDa

Reconstitution

It is recommended to reconstitute the lyophilized GCSF in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

The ED₅₀ determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05ng/ml, corresponding to a specific activity of greater than 2.0× 10⁷ IU/mg.

Reconstitution

It is recommended to reconstitute the lyophilized GCSF in sterile 18M-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For CSF3 (Source: Uniprot.org, NCBI)

Alternative Names

Granulocyte colony stimulating factor; Protein Csf3; Csf3 CSF3 C17orf33OS, GCSF, CSF3 colony stimulating factor 3 granulocyte colony-stimulating factor|filgrastim|granulocyte-colony stimulating factor|lenograstim|pluripoietin

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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