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Product Info Summary
| SKU: | PA1497 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CAMKK2 Antibody Picoband®
SKU/Catalog Number
PA1497
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CAMKK2 Antibody catalog # PA1497. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CAMKK2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1497)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human CAMKK2, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1497 is reactive to CAMKK2 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
64746 MW
Background of CAMKK2
CAMKK2, Calcium/calmodulin-dependent protein kinase kinase 2, is an enzyme that in humans is encoded by the CAMKK2 gene. The product of this gene belongs to the serine/threonine-specific protein kinase family, and to the Ca++/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. CaMKK2 regulates production of the appetite stimulating hormone neuropeptide Y and functions as an AMPK kinase in the hypothalamus. It also has an important role in the development of hyperalgesia and tolerance to opioid analgesic drugs, through reduction in downstream signalling pathways and mu opioid receptor downregulation. Inhibition of CaMKK2 in mice reduces appetite and promotes weight loss.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1497 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Rat Brain Tissue, Mouse Brain Tissue, Mouse Brain Tissue
IHC: Rat Brain Tissue
Validation Images & Assay Conditions
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Anti-CAMKK2 antibody, PA1497, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: Mouse Brain Tissue Lysate
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Anti-CAMKK2 antibody, PA1497, IHC(P)
IHC(P): Rat Brain Tissue
Protein Target Info & Infographic
Gene/Protein Information For CAMKK2 (Source: Uniprot.org, NCBI)
Gene Name
CAMKK2
Full Name
Calcium/calmodulin-dependent protein kinase kinase 2
Weight
64746 MW
Superfamily
protein kinase superfamily
Alternative Names
Calcium/calmodulin-dependent protein kinase kinase 2;CaM-KK 2;CaM-kinase kinase 2;CaMKK 2;2.7.11.17;Calcium/calmodulin-dependent protein kinase kinase beta;CaM-KK beta;CaM-kinase kinase beta;CaMKK beta;CAMKK2;CAMKKB, KIAA0787; CAMKK2 CAMKK, CAMKKB calcium/calmodulin dependent protein kinase kinase 2 calcium/calmodulin-dependent protein kinase kinase 2|CAMKK beta protein|caM-KK 2|caM-KK beta|caM-kinase kinase 2|caM-kinase kinase beta|calcium/calmodulin-dependent protein kinase beta|calcium/calmodulin-dependent protein kinase kinase 2, beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CAMKK2, check out the CAMKK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CAMKK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CAMKK2 Antibody Picoband® (PA1497)
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No publications found for PA1497
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Recommended Resources
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-CAMKK2 Antibody Picoband®
Question
We are currently using anti-CAMKK2 antibody PA1497 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
The anti-CAMKK2 antibody (PA1497) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-22
Question
My team were satisfied with the WB result of your anti-CAMKK2 antibody. However we have seen positive staining in cervix carcinoma nucleus. using this antibody. Is that expected? Could you tell me where is CAMKK2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
From literature, cervix carcinoma does express CAMKK2. Generally CAMKK2 expresses in nucleus. Regarding which tissues have CAMKK2 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 9872452
Brain cortex, Pubmed ID: 9822657
Cervix carcinoma, Pubmed ID: 18669648, 18691976
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334
Stomach, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-09-19
Question
We have been able to see staining in rat stomach. Do you have any suggestions? Is anti-CAMKK2 antibody supposed to stain stomach positively?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
Based on literature stomach does express CAMKK2. Based on Uniprot.org, CAMKK2 is expressed in cerebellum, brain cortex, brain, stomach, placenta, amygdala, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have CAMKK2 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 9872452
Brain cortex, Pubmed ID: 9822657
Cervix carcinoma, Pubmed ID: 18669648, 18691976
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334
Stomach, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-05-28
Question
Our lab want to know about using your anti-CAMKK2 antibody for positive regulation of protein phosphorylation studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-07-30
Answer
We appreciate your inquiry. This PA1497 anti-CAMKK2 antibody is tested on rat brain tissue, tissue lysate, mouse brain. It is guaranteed to work for IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-07-30
Question
We purchased anti-CAMKK2 antibody for IHC on cervix carcinoma erythroleukemia a few years ago. I am using mouse, and We intend to use the antibody for ICC next. Our lab want to know about examining cervix carcinoma erythroleukemia as well as placenta in our next experiment. Do you have any suggestion on which antibody would work the best for ICC?
B. Zhao
Verified customer
Asked: 2014-03-11
Answer
I have checked the website and datasheets of our anti-CAMKK2 antibody and it appears that PA1497 has been validated on mouse in both IHC and ICC. Thus PA1497 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-03-11
