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Product Info Summary
| SKU: | PA1237 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CXCR4 Antibody Picoband®
SKU/Catalog Number
PA1237
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CXCR4 Antibody catalog # PA1237. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CXCR4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1237)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CXCR4, different from the related rat and mouse sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1237 is reactive to CXCR4 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
40-43 kDa
Calculated molecular weight
39746 MW
Background of CXCR4
CXCR4 (Chemokine,CXC Motif, Receptor 4), also known as FUSIN or NPY3R, is a protein that in humans is encoded by the CXCR4 gene. It is the receptor for the CXC chemokine SDF1 that has essential functions on embryo organogenesis, immunological functions and T lymphocyte trafficking. CXCR4 is the only SDF1 receptor identified so far. This suggests that CXCR4 expression is critical for the biological effects of SDF1. CXCR4 is also a seven-transmembrane-spanning, G-protein-coupled receptor for the CXC chemokine PBSF/SDF-1. It functions as a co-receptor for T-cell-line tropic human immunodeficiency virus HIV-1. It was concluded that PBSF/SDF-1 and CXCR4 define a new signalling system for organ vascularization.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1237 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: human THP-1 whole cell, human Hela whole cell
IHC: human mammary cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CXCR4 using anti-CXCR4 antibody (PA1237).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human Hela whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR4 antigen affinity purified polyclonal antibody (Catalog # PA1237) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR4 at approximately 40-43KD. The expected band size for CXCR4 is at 40KD.
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Figure 2. IHC analysis of CXCR4 using anti-CXCR4 antibody (PA1237).
CXCR4 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CXCR4 Antibody (PA1237) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CXCR4 (Source: Uniprot.org, NCBI)
Gene Name
CXCR4
Full Name
C-X-C chemokine receptor type 4
Weight
39746 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
C-X-C chemokine receptor type 4;CXC-R4;CXCR-4;FB22;Fusin;HM89;LCR1;Leukocyte-derived seven transmembrane domain receptor;LESTR;Lipopolysaccharide-associated protein 3;LAP-3;LPS-associated protein 3;NPYRL;Stromal cell-derived factor 1 receptor;SDF-1 receptor;CD184;CXCR4; CXCR4 CD184, D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, WHIMS C-X-C motif chemokine receptor 4 C-X-C chemokine receptor type 4|CD184 |LPS-associated protein 3|SDF-1 receptor|chemokine (C-X-C motif) receptor 4|fusin|leukocyte-derived seven transmembrane domain receptor|lipopolysaccharide-associated protein 3|neuropeptide Y receptor Y3|neuropeptide Y3 receptor|seven transmembrane helix receptor|seven-transmembrane-segment receptor, spleen|stromal cell-derived factor 1 receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CXCR4, check out the CXCR4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CXCR4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CXCR4 Antibody Picoband® (PA1237)
Hello CJ!
PA1237 has been cited in 28 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
CXCL12 induces migration of oligodendrocyte precursor cells through the CXCR4‑activated MEK/ERK and PI3K/AKT pathways
CXCR4 protects bone marrow‑derived endothelial progenitor cells against hypoxia through the PI3K/Akt signaling pathway
Forkhead box P3 gene silencing inhibits the expression of chemokines and chemokine receptors associated with cell growth, migration, and apoptosis in hepatocellular carcinoma cells
Effect of CO2 pneumoperitoneum on the expression of the chemokine receptors CXCR4 and CCR7 in colorectal carcinoma cellsin vitro
CXCR4/SDF-1 axis is involved in lymph node metastasis of gastric carcinoma
Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer
Overexpression of CXCR4 in tracheal epithelial cells promotes their proliferation and migration to a stromal cell-derived factor-1 gradient:
Demineralized Bone Scaffolds with Tunable Matrix Stiffness for Efficient Bone Integration
Preferential siRNA delivery to injured kidneys for combination treatment of acute kidney injury
SDF-1α/CXCR4-mediated migration of systemically transplanted bone marrow stromal cells towards ischemic brain lesion in a rat model
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-CXCR4 Antibody Picoband®
Question
Do you have a BSA free version of anti-CXCR4 antibody PA1237 available?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CXCR4 antibody PA1237 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-05-01
Question
Our lab used your anti-CXCR4 antibody for IHC on oviduct epithelium in the past. I am using rat, and We intend to use the antibody for WB next. I was wanting to use examining oviduct epithelium as well as monocyte in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
I took a look at the website and datasheets of our anti-CXCR4 antibody and it appears that PA1237 has been validated on rat in both IHC and WB. Thus PA1237 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-21
Question
I am looking for using your anti-CXCR4 antibody for brain development studies. Has this antibody been tested with western blotting on human hela? We would like to see some validation images before ordering.
E. Evans
Verified customer
Asked: 2020-04-07
Answer
Thank you for your inquiry. This PA1237 anti-CXCR4 antibody is validated on human hela, hela whole cell lysates. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-07
Question
Is a blocking peptide available for product anti-CXCR4 antibody (PA1237)?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
We do provide the blocking peptide for product anti-CXCR4 antibody (PA1237). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-25
Question
I see that the anti-CXCR4 antibody PA1237 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-26
Question
I was wanting to use your anti-CXCR4 antibody for IHC for human monocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human monocyte identification?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
It shows on the product datasheet, PA1237 anti-CXCR4 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human monocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-21
Question
I am interested in to test anti-CXCR4 antibody PA1237 on human monocyte for research purposes, then I may be interested in using anti-CXCR4 antibody PA1237 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
The products we sell, including anti-CXCR4 antibody PA1237, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-19
Question
Please see the WB image, lot number and protocol we used for monocyte using anti-CXCR4 antibody PA1237. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-18
Question
Does anti-CXCR4 antibody PA1237 work for IHC with monocyte?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
According to the expression profile of monocyte, CXCR4 is highly expressed in monocyte. So, it is likely that anti-CXCR4 antibody PA1237 will work for IHC with monocyte.
Boster Scientific Support
Answered: 2019-07-22
Question
Is this PA1237 anti-CXCR4 antibody reactive to the isotypes of CXCR4?
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
The immunogen of PA1237 anti-CXCR4 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human CXCR4(18-33aa SGDYDSMKEPCFREEN), different from the related rat and mouse sequences by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-17
Question
We are currently using anti-CXCR4 antibody PA1237 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
The anti-CXCR4 antibody (PA1237) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-21
Question
We have observed staining in human cervix carcinoma. Any tips? Is anti-CXCR4 antibody supposed to stain cervix carcinoma positively?
W. Banerjee
Verified customer
Asked: 2019-05-21
Answer
According to literature cervix carcinoma does express CXCR4. According to Uniprot.org, CXCR4 is expressed in oviduct epithelium, lung, fetal brain, fetal spleen, monocyte, peripheral blood leukocyte, peripheral blood lymphocyte, neutrophil, adrenal gland, colon, cervix carcinoma, among other tissues. Regarding which tissues have CXCR4 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18220336, 18669648, 20068231, 23186163
Colon, Pubmed ID: 15489334
Fetal brain, Pubmed ID: 8234909
Fetal spleen, Pubmed ID: 8325644
Lung, Pubmed ID: 8329116, 15815621
Monocyte, Pubmed ID: 8276799
Boster Scientific Support
Answered: 2019-05-21
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for monocyte using anti-CXCR4 antibody PA1237. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-12-19
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-19
Question
My team were well pleased with the WB result of your anti-CXCR4 antibody. However we have seen positive staining in peripheral blood leukocyte cell membrane using this antibody. Is that expected? Could you tell me where is CXCR4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-05-10
Answer
Based on literature, peripheral blood leukocyte does express CXCR4. Generally CXCR4 expresses in cell membrane. Regarding which tissues have CXCR4 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18220336, 18669648, 20068231, 23186163
Colon, Pubmed ID: 15489334
Fetal brain, Pubmed ID: 8234909
Fetal spleen, Pubmed ID: 8325644
Lung, Pubmed ID: 8329116, 15815621
Monocyte, Pubmed ID: 8276799
Boster Scientific Support
Answered: 2018-05-10
Question
My question regarding product PA1237, anti-CXCR4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-10-13
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1237 anti-CXCR4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-13
Question
Does PA1237 anti-CXCR4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
F. Jackson
Verified customer
Asked: 2014-06-18
Answer
It shows on the product datasheet, PA1237 anti-CXCR4 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-06-18
