Anti-Human TCP1 alpha DyLight® 488 conjugated Antibody(monoclonal, 2E7)

Product Name

Anti-Human TCP1 alpha DyLight® 488 conjugated Antibody(monoclonal, 2E7)

View all TCP1 alpha Antibodies

SKU/Catalog Number

M02389-Dyl488

Size

100 μg/vial

Form

Liquid

Description

Boster Bio Anti-Human TCP1 alpha DyLight® 488 conjugated Antibody (monoclonal, 2E7) catalog # M02389-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Cite This Product

Anti-Human TCP1 alpha DyLight® 488 conjugated Antibody(monoclonal, 2E7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02389-Dyl488)

Host

Mouse

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Clonality

Monoclonal

Clone Number

Clone: 2E7

Isotype

Mouse IgG1

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human TCP1 alpha, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M02389-Dyl488 is reactive to TCP1 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Observed Molecular Weight

39 kDa

Calculated molecular weight

60.344kDa

Background of TCP1 alpha

T-complex protein 1 subunit alpha is a protein that in humans is encoded by the TCP1 gene. The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M02389-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells

Validation Images & Assay Conditions

Gene/Protein Information For TCP1 (Source: Uniprot.org, NCBI)

Alternative Names

T-complex protein 1 subunit alpha; TCP-1-alpha; CCT-alpha; TCP1; CCT1; CCTA TCP1 CCT-alpha, CCT1, CCTa, D6S230E, TCP-1-alpha t-complex 1 T-complex protein 1 subunit alpha|T-complex protein 1, alpha subunit|t-complex 1 protein|tailless complex polypeptide 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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