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Product Info Summary
| SKU: | EK0513-BV |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Bovine |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (EDTA) and urine. |
Product info
Product Name
Bovine TGF Beta 1 ELISA Kit PicoKine®
SKU/Catalog Number
EK0513-BV
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Bovine TGF Beta 1 ELISA Kit PicoKine® (96 Tests). Quantitate Bovine TGFB1 in cell culture supernatants, serum, plasma (EDTA) and urine. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Bovine TGF Beta 1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0513-BV)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: A279-S390
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0513-BV for more details
Cross-reactivity
There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%.
Reactive Species
EK0513-BV is reactive to TGFB1 in Bovine samples
Validated Sample Types
cell culture supernatants, serum, plasma (EDTA) and urine.
Application Guarantee
EK0513-BV is guaranteed for ELISA in Bovine by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TGFB1
Transforming growth factor-beta1 (TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects.TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation.TGFbeta1 is also an important mediator of bone remodeling.TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation.TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0513-BV-CAP | Anti-Bovine TGFB1 Pre-coated 96-well strip microplate | 1 |
| EK0513-BV-ST | Bovine TGFB1 Standard | 2 vials, 10 ng/tube |
| EK0513-BV-DA | Bovine TGFB1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.075 | 0.136 | 0.229 | 0.310 | 0.462 | 0.770 | 1.192 | 1.837 |
Data Example Images
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Bovine TGF beta 1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Bovine TGF Beta 1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 38 | 184 | 433 | 36 | 179 | 490 |
| Standard deviation | 2.09 | 8.46 | 30.74 | 2.45 | 10.56 | 42.14 |
| CV (%) | 5.5% | 4.6% | 7.1% | 6.8% | 5.9% | 8.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TGFB1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 38 | 38 | 35 | 34 | 36 | 1.78 | 4.9% |
| Sample 2 | 184 | 175 | 191 | 162 | 178 | 10.83 | 6% |
| Sample 3 | 433 | 417 | 480 | 413 | 435 | 26.62 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For TGFB1 (Source: Uniprot.Org, NCBI)
Gene Name
TGFB1
Full Name
Transforming growth factor beta-1 proprotein
Weight
44341 MW
Superfamily
TGF-beta family
Alternative Names
Transforming growth factor beta-1;TGF-beta-1;Latency-associated peptide;LAP;TGFB1;TGFB; TGFB1 CED, DPD1, IBDIMDE, LAP, TGF-beta1, TGFB, TGFbeta transforming growth factor beta 1 transforming growth factor beta-1 proprotein|TGF-beta-1|latency-associated peptide|prepro-transforming growth factor beta-1|transforming growth factor beta1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TGFB1, check out the TGFB1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TGFB1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Bovine TGF Beta 1 ELISA Kit PicoKine® (EK0513-BV)
Hello CJ!
EK0513-BV has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Cell immaturity and white/beige adipocyte potential of primary human adipose‐derived stromal cells are restrained by culture‐medium TGFβ1
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Bovine TGF Beta 1 ELISA Kit PicoKine®
Question
Has EK0513-BV been tested on platelet lysate?
Verified customer
Asked: 2020-12-22
Answer
Unfortunately, the Bovine TGF Beta 1 ELISA Kit PicoKine™ (EK0513-BV) hasn't been tested on platelet lysate. Please run pilot tests to see if they could work.
Boster Scientific Support
Answered: 2020-12-23
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of TGFB1 in Bovine serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-11-28
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TGFB1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-11-28
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
B. Kaur
Verified customer
Asked: 2020-09-17
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-09-17
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement TGFB1 using the EK0513-BV Bovine TGFB1 Picokine® ELISA Kit?
R. Martinez
Verified customer
Asked: 2020-08-26
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2020-08-26
Question
Q: how to analyze ELISA data? I have performed TGFB1 level in serum.
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-04-03
Question
Q: What is the optimal O.D. value for TGFB1 ELISA kit? I performed your TGFB1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain TGFB1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-12-30
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TGFB1 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of TGFB1 that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2018-12-30
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-06-25
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-06-25
Question
Q: if the enzyme conjugated TGFB1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TGFB1 antigen?
Verified Customer
Verified customer
Asked: 2018-02-21
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-02-21
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-06-09
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-06-09
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2015-10-22
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2015-10-22
