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Key=sku, value=EK0513-CN
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Key=created_at, value=2016-09-16 02:59:46
Key=updated_at, value=2024-10-14 05:03:36
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Key=status, value=Enabled
Key=name, value=Dog Canine TGF Beta 1 ELISA Kit PicoKine®
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Key=meta_title, value=Dog Canine TGF Beta 1 ELISA Kit Price PicoKine® | BosterBio
Key=meta_description, value=Dog Canine TGF Beta 1 ELISA Kit PicoKine® (96 Tests). Quantitate Dog TGFB1 in cell culture supernatants, serum, plasma (EDTA) and urine.. Sensitivity: 1pg/ml.
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Key=url_key, value=dog-canine-tgf-beta-1-picokine-trade-elisa-kit-ek0513-cn-boster
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Key=image_label, value=Dog TGF beta 1 PicoKine ELISA Kit standard curve
Key=small_image_label, value=Dog TGF beta 1 PicoKine ELISA Kit standard curve
Key=thumbnail_label, value=Dog TGF beta 1 PicoKine ELISA Kit standard curve
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Key=size, value=96 wells/kit, with removable strips.
Key=uniprot_id, value=P54831
Key=assay_range, value=15.6 pg/ml - 1,000 pg/ml
Key=sensitivity, value=<1 pg/ml
Key=applications, value=ELISA
Key=reactivity, value=Canine
Key=cross_reactivity, value=There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%.
Key=principle, value=polyclonal antibody from chicken|polyclonal antibody from chicken
Key=anticoagulant, value=EDTA
Key=assay_procedure_step_1, value=1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml, 15.6 pg/ml
Key=tmb_incubation_time, value=15-25min
Key=normal_range_citations, value=21786325
Key=source_company, value=Boster EK0513
Key=molecular_weight, value=44341 MW
Key=related_product_skus, value=M00019,PROTP01137,PROTP01137-1,PROTP01137-2,PROTP01137-3,PROTP01137-4,PROTP01137-5,PROTP04202
Key=product_category, value=Picokine ELISA Kits
Key=research_category, value=Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Co-Factors, Cofactors, Vitamins/Minerals, Developmental Biology, Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Metabolism, Metabolism Processes, Organogenesis, Pathways and Processes, Response To Hypoxia, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, TGF Beta
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Key=cj_related_products, value=Fibroblast Proliferation:EZ1625^Hepatic Fibrosis:EK0513-PO|PA1385|PA1879|A01698-1^Pulmonary Fibrosis:A07954-1
Key=description, value=Dog Canine TGF Beta 1 ELISA Kit PicoKine® (96 Tests). Quantitate Dog TGFB1 in cell culture supernatants, serum, plasma (EDTA) and urine. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=short_description, value=Dog Canine TGF Beta 1 ELISA Kit PicoKine® (96 Tests). Quantitate Dog TGFB1 in cell culture supernatants, serum, plasma (EDTA) and urine. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=meta_keyword, value=Dog Canine TGF beta 1 ELISA Kit, LAP/TGF beta 1 ELISA, Bosterbio High Sensitivity
Key=background, value=Transforming growth factor-beta1 (TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects.TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation.TGFbeta1 is also an important mediator of bone remodeling.TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation.TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa.
Key=gene_name, value=TGFB1
Key=synonyms, value=Transforming growth factor beta-1;TGF-beta-1;Latency-associated peptide;LAP;TGFB1;TGFB;
Key=sample_type, value=cell culture supernatants, serum, plasma (EDTA) and urine.
Key=kit_components, value=10 ng/tube
Key=componentsneeded, value=

• Microplate reader in standard size.
• Automated plate washer.
• Incubator.
• Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
• Clean tubes and Eppendorf tubes.
• Washing buffer (neutral PBS or TBS).
• Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
• Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Key=intra_inter_assay_cv, value=

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	135	264	521	152	270	543
Standard deviation	3.64	13.73	18.8	7.6	17.3	32.04
CV (%)	4.4	5.2	3.6	5	6.4	5.9

Key=subcellular_localization, value= Secreted, extracellular space, extracellular matrix .
Key=sequence_similarities, value=Belongs to the TGF-beta family.
Key=tissue_specificity, value= Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Colocalizes with ASPN in chondrocytes within OA lesions of articular cartilage. .
Key=sample_data, value=

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.039	0.108	0.186	0.321	0.599	1.017	1.586	2.000

Key=activating_reagent, value=TGF beta 1 is mostly contained as inactive form in samples, please activate it before assay. Don't activate recombinant TGF beta 1.
Solution A: 1N HCI: add 8.33ml of 12N HCI into 91.67ml of H₂O.
Solution B: 1.2N NaOH/0.5M HEPES: add 12ml of 10N NaOH and 11.9g HEPES into 75ml of H₂O, add H₂O to adjust volume to 100ml.
Key=sample_activation_procedure, value=

Activate the sample

Cell culture supernate, urine: add activating reagent pro rata, i.e. add 20ul of Solution A into 100ul of sample, 10 min later, add 20ul of Solution B. PH 7.0-7.6.

Serum, plasma (EDTA): add activating reagent pro rata, i.e. add 20ul of Solution A into 40l of sample, 10 min later, add 20ul of Solution B. PH 7.0-7.6.

It is unnecessary to activate the recombinant TGF 1.

Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration.

Key=protein_function, value= Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T- helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. .
Key=competitor_equivalent_skus, value=
Key=immunogen, value=Expression system for standard: CHO; Immunogen sequence: A279-S390
Key=storage, value=Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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