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Product Info Summary
| SKU: | EK0928 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA, citrate) , Cell lysates, and urine. |
Product info
Product Name
Human Tissue Factor/F3 ELISA Kit PicoKine®
View all Coagulation Factor III/Tissue Factor ELISA kits
SKU/Catalog Number
EK0928
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Tissue Factor/F3 ELISA Kit PicoKine® (96 Tests). Quantitate Human F3 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) , Cell lysates, and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Tissue Factor/F3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0928)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: S33-E251
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0928 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0928 is reactive to F3 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA, citrate) , Cell lysates, and urine.
Application Guarantee
EK0928 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Coagulation Factor III/Tissue Factor
Tissue factor, also called platelet tissue factor, factor III, thrombokinase, or CD142 is a protein present in sub endothelial tissue and leukocytes necessary for the initiation of thrombin formation from the zymogene prothrombin. An incorrect synonym is thromboplastin. the F3 gene was mapped to 1pter-p21 by study of somatic cell hybrids with a species-specific sensitive chromogenic assay. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0928-CAP | Anti-Human F3 Pre-coated 96-well strip microplate | 1 |
| EK0928-ST | Human F3 Standard | 2 vials, 10 ng/tube |
| EK0928-DA | Human F3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D | 0.031 | 0.101 | 0.165 | 0.296 | 0.708 | 0.934 | 1.851 | 2.431 |
Data Example Images
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Human Tissue factor/F3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Coagulation Factor III/Tissue Factor, Dilution ratio of 1:1, concentration in serum and plasma is around 40 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Tissue Factor/F3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 28 | 204 | 455 | 28 | 205 | 493 |
| Standard deviation | 1.26 | 13.46 | 34.58 | 1.54 | 14.14 | 40.91 |
| CV (%) | 4.5% | 6.6% | 7.6% | 5.5% | 6.9% | 8.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Coagulation Factor III/Tissue Factor in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 28 | 29 | 31 | 27 | 28 | 1.47 | 5.2% |
| Sample 2 | 204 | 231 | 201 | 203 | 209 | 12.31 | 5.8% |
| Sample 3 | 455 | 425 | 389 | 431 | 425 | 23.62 | 5.5% |
Protein Target Info & Infographic
Gene/Protein Information For F3 (Source: Uniprot.Org, NCBI)
Gene Name
F3
Full Name
Tissue factor
Weight
33068 MW
Superfamily
tissue factor family
Alternative Names
Tissue factor;TF;Coagulation factor III;Thromboplastin;CD142;F3; F3 CD142, TF, TFA coagulation factor III, tissue factor tissue factor|coagulation factor III (thromboplastin, tissue factor)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on F3, check out the F3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for F3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Tissue Factor/F3 ELISA Kit PicoKine® (EK0928)
Hello CJ!
EK0928 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Evaluation of venous thrombosis and tissue factor in epithelial ovarian cancer
Cohen, J.G., Prendergast, E., Geddings, J.E., Walts, A.E., Agadjanian, H., Hisada, Y.,…& Walsh, C.S. (2017). Evaluation of venous thrombosis and tissue factor in epithelial ovarian cancer. Gynecologic Oncology. Advance online publication. doi: 10....
Chen J, Wei D, Zhuang H, Qiao Y, Tang B, Zhang X, Wei J, Fang S, Chen G, Du P, Huang X, Jiang W, Hu Q, Hua Zc. Mol Cell Proteomics. 2011 Jun;10(6):M111.009399. Doi: 10.1074/Mcp.M111.009399. Epub 2011 Apr 7. Proteomic Screening Of Anaerobically Reg...
Wu Z, Ming Y, Zhang Z, Wu J, Wu Z, Yao S. Chin Med J (Engl). 2014;127(5):968-70. Edaravone Rescues The Lung By Inhibiting Lipid Peroxidation And Pro-Inflammatory Cytokines In A Rat Model.
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Human Tissue Factor/F3 ELISA Kit PicoKine®
Question
Q: how can I thaw whole blood sample for Tissue Factor ELISA after freezing?
M. Williams
Verified customer
Asked: 2020-12-06
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Tissue Factor for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-12-06
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-11-12
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-11-12
Question
Can we measure the concentration of recombinant tissue factor, for both natural and recombinant?
Verified customer
Asked: 2020-06-09
Answer
The expression system of standard (recombinant protein) was NSO eukaryotic expression system. If the recombinant tissue factor was expressed using eukaryotic expression system, the Human Tissue Factor/F3 ELISA Kit PicoKine™ (EK0928) will be more likely to detect it. If the recombinant tissue factor was expressed using prokaryotic expression system, the kit might be less likely to detect it. We suggest to run a pilot test to see if the kit will work.
Boster Scientific Support
Answered: 2020-06-17
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-12-17
Question
Q: can you suggest the dilution ratio of serum samples for detection of Tissue Factor in Human Cell lysates? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
W. Kulkarni
Verified customer
Asked: 2019-06-09
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Tissue Factor ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-06-09
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-02-27
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-02-27
Question
Q: how to proceed with the analysis of ELISA data? I have performed Tissue Factor level in Cell lysates.
Verified Customer
Verified customer
Asked: 2019-01-29
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-01-29
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-11-19
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-11-19
Question
Q: if the enzyme conjugated Tissue Factor antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Tissue Factor antigen?
R. Parker
Verified customer
Asked: 2018-04-16
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-04-16
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Tissue Factor using the EK0928 Human Tissue Factor Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-01-15
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2018-01-15
