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Product Info Summary
| SKU: | A12956-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-TOX2 Antibody Picoband®
SKU/Catalog Number
A12956-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TOX2 Antibody Picoband® catalog # A12956-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TOX2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12956-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TOX2 recombinant protein (Position: Q41-A425).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A12956-1 is reactive to TOX2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
70 kDa
Calculated molecular weight
51.604kDa
Background of TOX2
TOX high mobility group box family member 2, also known as TOX2, is a human gene. Enables transcription coactivator activity. Involved in positive regulation of transcription by RNA polymerase II. Located in nucleoplasm.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12956-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat thymus tissue, rat liver tissue, mouse thymus tissue, mouse liver tissue
ICC/IF: A549 cell
FCM: MCF-7 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TOX2 using anti-TOX2 antibody (A12956-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse thymus tissue lysates,
Lane 4: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOX2 antigen affinity purified polyclonal antibody (Catalog # A12956-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOX2 at approximately 70 kDa. The expected band size for TOX2 is at 52 kDa.
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Figure 2. IF analysis of TOX2 using anti-TOX2 antibody (A12956-1).
TOX2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TOX2 Antibody (A12956-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of MCF-7 cells using anti-TOX2 antibody (A12956-1).
Overlay histogram showing MCF-7 cells stained with A12956-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TOX2 Antibody (A12956-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For TOX2 (Source: Uniprot.org, NCBI)
Gene Name
TOX2
Full Name
TOX high mobility group box family member 2
Weight
51.604kDa
Alternative Names
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8; CPAMD8; KIAA1283 TOX2 C20orf100, GCX-1, GCX1, dJ1108D11.2, dJ495O3.1 TOX high mobility group box family member 2 TOX high mobility group box family member 2|granulosa cell HMG box 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TOX2, check out the TOX2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TOX2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TOX2 Antibody Picoband® (A12956-1)
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