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Product Info Summary
| SKU: | M00953-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Topoisomerase II alpha TOP2A Rabbit Monoclonal Antibody
SKU/Catalog Number
M00953-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Topoisomerase II alpha TOP2A Rabbit Monoclonal Antibody catalog # M00953-1. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Topoisomerase II alpha TOP2A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00953-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FB-20
Isotype
Rabbit IgG
Standard Protein
A synthesized peptide derived from human Topoisomerase II alpha
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00953-1 is reactive to TOP2A in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
28 kDa
Calculated molecular weight
174385 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00953-1 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
Positive Control
WB: HeLa cell, Jurkat cell
IHC: human colon cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of Topoisomerase II alpha expression in (1) HeLa cell lysate; (2) Jurkat cell lysate.
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Immunohistochemical analysis of paraffin-embedded human colon cancer, using Topoisomerase II alpha Antibody.
Protein Target Info & Infographic
Gene/Protein Information For TOP2A (Source: Uniprot.org, NCBI)
Gene Name
TOP2A
Full Name
DNA topoisomerase 2-alpha
Weight
174385 MW
Superfamily
type II topoisomerase family
Alternative Names
DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase 2-alpha; DNA topoisomerase II, 170 kD; DNA topoisomerase II, alpha isozyme; EC 5.99.1.3; TOP2; TOP2A; TOP2DNA gyrase; topoisomerase (DNA) II alpha (170kD); topoisomerase (DNA) II alpha 170kDa; TP2A TOP2A TOP2, TP2A DNA topoisomerase II alpha DNA topoisomerase 2-alpha|DNA gyrase|DNA topoisomerase (ATP-hydrolyzing)|DNA topoisomerase II, 170 kD|DNA topoisomerase II, alpha isozyme|topoisomerase (DNA) II alpha 170kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TOP2A, check out the TOP2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TOP2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Topoisomerase II alpha TOP2A Rabbit Monoclonal Antibody (M00953-1)
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No publications found for M00953-1
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Topoisomerase II alpha TOP2A Rabbit Monoclonal Antibody
Question
We have tried in the past anti-Topoisomerase II alpha Rabbit Monoclonal antibody for WB on heart in the past. I am using rat, and We intend to use the antibody for IHC next. I would like examining heart as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
I took a look at the website and datasheets of our anti-Topoisomerase II alpha Rabbit Monoclonal antibody and it seems that M00953-1 has been validated on rat in both WB and IHC. Thus M00953-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-30
Question
We were content with the WB result of your anti-Topoisomerase II alpha Rabbit Monoclonal antibody. However we have observed positive staining in leukemic t-cell cytoplasm. using this antibody. Is that expected? Could you tell me where is TOP2A supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
From literature, leukemic t-cell does express TOP2A. Generally TOP2A expresses in cytoplasm. Regarding which tissues have TOP2A expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 10942766, 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2019-10-04
Question
I am interested in using your anti-Topoisomerase II alpha Rabbit Monoclonal antibody for hematopoietic progenitor cell differentiation studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
E. Zhao
Verified customer
Asked: 2019-04-12
Answer
We appreciate your inquiry. This M00953-1 anti-Topoisomerase II alpha Rabbit Monoclonal antibody is validated on hela cell lysate, jurkat cell lysate. It is guaranteed to work for IP, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-04-12
Question
We have seen staining in human ovarian carcinoma. Do you have any suggestions? Is anti-Topoisomerase II alpha Rabbit Monoclonal antibody supposed to stain ovarian carcinoma positively?
Verified Customer
Verified customer
Asked: 2017-06-06
Answer
Based on literature ovarian carcinoma does express TOP2A. Based on Uniprot.org, TOP2A is expressed in heart, ovarian carcinoma, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have TOP2A expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 10942766, 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2017-06-06
Question
We are currently using anti-Topoisomerase II alpha Rabbit Monoclonal antibody M00953-1 for rat tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-02
Answer
The anti-Topoisomerase II alpha Rabbit Monoclonal antibody (M00953-1) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-02
