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Product Info Summary
| SKU: | PA1127 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Topoisomerase II alpha/TOP2A Antibody Picoband®
SKU/Catalog Number
PA1127
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Topoisomerase II alpha/TOP2A Antibody catalog # PA1127. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Topoisomerase II alpha/TOP2A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1127)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Topoisomerase II alpha, different from the related rat sequence by three amino acids, and different from the related mouse sequence by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1127 is reactive to TOP2A in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
174 kDa
Calculated molecular weight
174385 MW
Background of TOP2A
The human topoisomerase II enzyme is encoded by a single-copy gene which is mapped to 17q21-q22. The TOP2A gene spans approximately 30 kb and contains 35 exons. Furthermore, DNA topoisomerases are enzymes that control and alter the topologic states of DNA in both prokaryotes and eukaryotes. Topoisomerase II from eukaryotic cells catalyzes the relaxation of supercoiled DNA molecules, catenation, decatenation, knotting, and unknotting of circular DNA. It appears likely that the reaction catalyzed by topoisomerase II involves the crossing-over of 2 DNA segments. There are about 100,000 molecules of topoisomerase II per HeLa cell nucleus, constituting about 0.1% of the nuclear extract. DNA topoisomerase II-alpha is associated with the pol II holoenzyme and is a required component of chromatin-dependent coactivation. Specific inhibitors of topoisomerase II blocked transcription on chromatin templates, but did not affect transcription on naked templates. Addition of purified topoisomerase II-alpha reconstituted chromatin-dependent activation activity in reactions with core pol II.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1127 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Whole Cell, JURKAT Whole Cell
Validation Images & Assay Conditions
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Anti-Topoisomerase II alpha antibody, PA1127, Western blotting
All lanes: Anti Topoisomerase II alpha (PA1127) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 174KD
Observed bind size: 174KD
Protein Target Info & Infographic
Gene/Protein Information For TOP2A (Source: Uniprot.org, NCBI)
Gene Name
TOP2A
Full Name
DNA topoisomerase 2-alpha
Weight
174385 MW
Superfamily
type II topoisomerase family
Alternative Names
DNA topoisomerase 2-alpha;5.99.1.3;DNA topoisomerase II, alpha isozyme;TOP2A;TOP2; TOP2A TOP2, TP2A DNA topoisomerase II alpha DNA topoisomerase 2-alpha|DNA gyrase|DNA topoisomerase (ATP-hydrolyzing)|DNA topoisomerase II, 170 kD|DNA topoisomerase II, alpha isozyme|topoisomerase (DNA) II alpha 170kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TOP2A, check out the TOP2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TOP2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Topoisomerase II alpha/TOP2A Antibody Picoband® (PA1127)
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No publications found for PA1127
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Topoisomerase II alpha/TOP2A Antibody Picoband®
Question
See below the WB image, lot number and protocol we used for heart using anti-Topoisomerase II alpha/TOP2A antibody PA1127. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-16
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart using anti-Topoisomerase II alpha/TOP2A antibody PA1127. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-31
Question
I have a question about product PA1127, anti-Topoisomerase II alpha/TOP2A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1127 anti-Topoisomerase II alpha/TOP2A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-24
Question
Will anti-Topoisomerase II alpha/TOP2A antibody PA1127 work for WB with heart?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
According to the expression profile of heart, TOP2A is highly expressed in heart. So, it is likely that anti-Topoisomerase II alpha/TOP2A antibody PA1127 will work for WB with heart.
Boster Scientific Support
Answered: 2019-10-09
Question
My team were happy with the WB result of your anti-Topoisomerase II alpha/TOP2A antibody. However we have seen positive staining in heart cytoplasm. using this antibody. Is that expected? Could you tell me where is TOP2A supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
Based on literature, heart does express TOP2A. Generally TOP2A expresses in cytoplasm. Regarding which tissues have TOP2A expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 10942766, 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2019-10-01
Question
Is this PA1127 anti-Topoisomerase II alpha/TOP2A antibody reactive to the isotypes of TOP2A?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
The immunogen of PA1127 anti-Topoisomerase II alpha/TOP2A antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Topoisomerase II alpha(1466-1480aa RKPSTSDDSDSNFEK), different from the related rat sequence by three amino acids, and different from the related mouse sequence by four amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-15
Question
We want using your anti-Topoisomerase II alpha/TOP2A antibody for regulation of circadian rhythm studies. Has this antibody been tested with western blotting on jurkat whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
We appreciate your inquiry. This PA1127 anti-Topoisomerase II alpha/TOP2A antibody is tested on hela whole cell lysate, jurkat whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-26
Question
I was wanting to use your anti-Topoisomerase II alpha/TOP2A antibody for WB for human heart on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human heart identification?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
As indicated on the product datasheet, PA1127 anti-Topoisomerase II alpha/TOP2A antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human heart in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-22
Question
I see that the anti-Topoisomerase II alpha/TOP2A antibody PA1127 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-06-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-06-04
Question
Our lab want to know about to test anti-Topoisomerase II alpha/TOP2A antibody PA1127 on human heart for research purposes, then I may be interested in using anti-Topoisomerase II alpha/TOP2A antibody PA1127 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-03-28
Answer
The products we sell, including anti-Topoisomerase II alpha/TOP2A antibody PA1127, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-28
Question
We have been able to see staining in human ovarian carcinoma. Any tips? Is anti-Topoisomerase II alpha/TOP2A antibody supposed to stain ovarian carcinoma positively?
Verified Customer
Verified customer
Asked: 2018-01-30
Answer
According to literature ovarian carcinoma does express TOP2A. According to Uniprot.org, TOP2A is expressed in heart, ovarian carcinoma, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have TOP2A expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 10942766, 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2018-01-30
Question
Is a blocking peptide available for product anti-Topoisomerase II alpha/TOP2A antibody (PA1127)?
Verified Customer
Verified customer
Asked: 2018-01-09
Answer
We do provide the blocking peptide for product anti-Topoisomerase II alpha/TOP2A antibody (PA1127). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-01-09
Question
Is there a BSA free version of anti-Topoisomerase II alpha/TOP2A antibody PA1127 available?
J. Mitchell
Verified customer
Asked: 2016-01-05
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Topoisomerase II alpha/TOP2A antibody PA1127 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-01-05
Question
Would PA1127 anti-Topoisomerase II alpha/TOP2A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Wu
Verified customer
Asked: 2014-06-26
Answer
As indicated on the product datasheet, PA1127 anti-Topoisomerase II alpha/TOP2A antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-06-26
Question
We are currently using anti-Topoisomerase II alpha/TOP2A antibody PA1127 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
T. Lewis
Verified customer
Asked: 2013-07-04
Answer
The anti-Topoisomerase II alpha/TOP2A antibody (PA1127) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-07-04
