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Product Info Summary
| SKU: | A07402 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Bag5 Antibody Picoband®
SKU/Catalog Number
A07402
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Bag5 Antibody Picoband® catalog # A07402. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bag5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07402)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Bag5 recombinant protein (Position: N389-Y447). Human Bag5 shares 96.6% and 93.2% amino acid (aa) sequence identity with mouse and rat Bag5, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07402 is reactive to BAG5 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
51 kDa
Calculated molecular weight
51200 MW
Background of BAG5
BAG family molecular chaperone regulator 5 is a protein that in humans is encoded by the BAG5 gene. It is mapped to 14q32.33. The protein encoded by this gene is a member of the BAG1-related protein family. Bag5 is a negative regulator of both Hsp70 and parkin function that sensitizes dopaminergic neurons to injury-induced death and thus promotes neurodegeneration.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07402 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human PC-3 whole cell, human U-87 whole cell, human Hela whole cell, human K562 whole cell, rat brain tissue, rat stomach tissue, mouse brain tissue, mouse stomach tissue
ICC/IF: T-47D cell
FCM: HL-60 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Bag5 using anti-Bag5 antibody (A07402).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human U-87 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat stomach tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse stomach tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bag5 antigen affinity purified polyclonal antibody (Catalog # A07402) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bag5 at approximately 51 kDa. The expected band size for Bag5 is at 51 kDa.
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Figure 3. Flow Cytometry analysis of HL-60 cells using anti-Bag5 antibody (A07402).
Overlay histogram showing HL-60 cells stained with A07402 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Bag5 Antibody (A07402, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 2. IF analysis of Bag5 using anti-Bag5 antibody (A07402).
Bag5 was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Bag5 Antibody (A07402) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For BAG5 (Source: Uniprot.org, NCBI)
Gene Name
BAG5
Full Name
BAG family molecular chaperone regulator 5
Weight
51200 MW
Alternative Names
BAG family molecular chaperone regulator 5;BAG-5;Bcl-2-associated athanogene 5;BAG5;KIAA0873; BAG5 BAG-5 BAG cochaperone 5 BAG family molecular chaperone regulator 5|BCL2 associated athanogene 5|bcl-2-associated athanogene 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BAG5, check out the BAG5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BAG5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bag5 Antibody Picoband® (A07402)
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Customer Q&As
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4 Customer Q&As for Anti-Bag5 Antibody Picoband®
Question
Does anti-Bag5 antibody A07402 work on monkey WB with testis?
B. Jones
Verified customer
Asked: 2017-02-16
Answer
Our lab technicians have not tested anti-Bag5 antibody A07402 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Bag5 antibody A07402 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-02-16
Question
I have a question about product A07402, anti-Bag5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Carter
Verified customer
Asked: 2016-10-13
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07402 anti-Bag5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-10-13
Question
Is there a BSA free version of anti-Bag5 antibody A07402 available?
A. Zhao
Verified customer
Asked: 2013-04-01
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Bag5 antibody A07402 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-04-01
Question
Here is the WB image, lot number and protocol we used for testis using anti-Bag5 antibody A07402. Please let me know if you require anything else.
M. Jha
Verified customer
Asked: 2013-01-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-01-03
