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Product Info Summary
| SKU: | EKC2591 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human |
| Application: | ELISA |
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Product info
Product Name
TOP2A (Phospho-Thr1343) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC2591
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The TOP2A (Phospho-Thr1343) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor TOP2A (Phospho-Thr1343) protein expression profile in cells. The kit can be used for measuring the relative amounts of TOP2A (Phospho-Thr1343) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on TOP2A (Phospho-Thr1343).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
TOP2A (Phospho-Thr1343) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2591)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2591 is reactive to TOP2A in Human samples
Application Guarantee
EKC2591 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-TOP2A Phospho-Thr1343 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-TOP2A Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from HepG2 cells treated with Ca2+ 40nM 30'
Protein Target Info & Infographic
Gene/Protein Information For TOP2A (Source: Uniprot.Org, NCBI)
Gene Name
TOP2A
Full Name
DNA topoisomerase 2-alpha
Weight
174385 MW
Superfamily
type II topoisomerase family
Alternative Names
DNA topoisomerase 2-alpha;5.99.1.3;DNA topoisomerase II, alpha isozyme;TOP2A;TOP2; TOP2A TOP2, TP2A DNA topoisomerase II alpha DNA topoisomerase 2-alpha|DNA gyrase|DNA topoisomerase (ATP-hydrolyzing)|DNA topoisomerase II, 170 kD|DNA topoisomerase II, alpha isozyme|topoisomerase (DNA) II alpha 170kDa
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TOP2A, check out the TOP2A Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TOP2A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For TOP2A (Phospho-Thr1343) Colorimetric Cell-Based ELISA Kit (EKC2591)
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