Anti-TLR4 Antibody

SKU PA1484
Size 100μg/vial
Reactivity Human
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications IHC, WB

Overview

Product Name Anti-TLR4 Antibody
SKU/Catalog Number PA1484
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Toll-like receptor 4(TLR4) detection. Tested with WB, IHC-P in Human.
Cite This Product Anti-TLR4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1484)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human TLR4(50-68aa DNLPFSTKNLDLSFNPLRH).
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Images And Assay Conditions

Anti-TLR4 antibody, PA1484, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: SMMC Cell Lysate

Anti-TLR4 antibody, PA1484, IHC(P)
IHC(P): Human Lung Cancer Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id O00206
Gene Name TLR4
Protein Name Toll-like receptor 4
Tissue Specificity Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
Alternative Names Toll-like receptor 4;hToll;CD284;TLR4;
Subcellular Localization Cell membrane; Single-pass type I membrane protein. Upon complex formation with CD36 and TLR6, internalized through dynamin-dependent endocytosis. .
Molecular Weight 95680 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS- independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+). Responses triggered by Ni(2+) require non-conserved histidines and are, therefore, species- specific. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. .
Research Areas Human

*You can search these to find other products in these research areas.
Background TLR4, Toll-like receptor 4, is a protein that in humans is encoded by the TLR4 gene. TLR 4 is a toll-like receptor. TLR4, the human homolog of Drosophila Toll, is a type I transmembrane protein with an extracellular domain consisting of a leucine-rich repeat region and an intracellular domain homologous to that of human interleukin-1 receptor. The TLR4 gene is mapped to chromosome 9q32-q33 by fluorescence in situ hybridization.It detects lipopolysaccharide from Gram-negative bacteria and is thus important in the activation of the innate immune system.The protein encoded by this gene is a member of the Toll-like receptor(TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns(PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity.

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In stock
Order Product
PA1484
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

Troubleshooting

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Publications

Rhein inhibits lipopolysaccharide-induced intestinal injury during sepsis by blocking the toll-like receptor 4 nuclear factor-%u03BAB pathway
Regulation on Toll-like Receptor 4 and Cell Barrier Function by Rab26 siRNA-loaded DNA Nanovector in Pulmonary Microvascular Endothelial Cells
Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4)-Mediated Signaling
Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response
Allicin Alleviates Reticuloendotheliosis Virus-Induced Immunosuppression via ERK/Mitogen-Activated Protein Kinase Pathway in Specific Pathogen-Free Chickens
Phospholipase A2 of Peroxiredoxin 6 Plays a Critical Role in Cerebral Ischemia/Reperfusion Inflammatory Injury
Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therapy Using the Bifidobacterium-Mediated Recombinant HSV-1 Thymidine Kinase and Ganciclovir
Knockdown of versicanV1 induces a severe inflammatory response in LPS-induced acute lung injury via the TLR2-NF-?B signaling pathway in C57BL/6J mice
Toll-like receptor 4 contributes to chronic itch, alloknesis, and spinal astrocyte activation in male mice
Inhibition of HMGB1 reduces rat spinal cord astrocytic swelling and AQP4 expression after oxygen-glucose deprivation and reoxygenation via TLR4 and NF-?B signaling in an IL-6-dependent manner
Expression of TLR2/4 in the sperm?storing oviduct of the Chinese soft?shelled turtle Pelodiscus sinensis during hibernation season
High glucose induces and activates Toll-like receptor 4 in endothelial cells of diabetic retinopathy
Increased Thymic Cell Turnover under Boron Stress May Bypass TLR3/4 Pathway in African Ostrich
Sun L, Xiu M, Wang S, Brigstock DR, Li H, Qu L, Gao R. J Cell Mol Med. 2018 Apr;22(4):2346-2356. doi: 10.1111/jcmm.13526. Epub 2018 Feb 9. Lipopolysaccharide enhances TGF‐β1 signalling pathway and rat pancreatic fibrosis
Wang Y, Ge P, Yang L, Wu C, Zha H, Luo T, Zhu Y. J Neuroinflammation. 2014 Jan 24;11:15. Doi: 10.1186/1742-2094-11-15. Protection Of Ischemic Post Conditioning Against Transient Focal Ischemia-Induced Brain Damage Is Associated With Inhibition Of ...
Tu Ct, Yao Qy, Xu Bl, Wang Jy, Zhou Ch, Zhang Sc. Food Chem Toxicol. 2012 Sep;50(9):3343-51. Doi: 10.1016/J.Fct.2012.05.050. Epub 2012 Jun 7. Protective Effects Of Curcumin Against Hepatic Fibrosis Induced By Carbon Tetrachloride: Modulation Of Hi...
Xu N, Yu Zh, Yao Qs, Wang Zq, Qu Hl, Sun Y, Liu J, Jin Cz. J Immunoassay Immunochem. 2013;34(3):219-31. Doi: 10.1080/15321819.2012.699490. Ppar-?? And Tollip Are Associated With Toll-Like Receptors In Colitis Rats.
Fu Hy, Li C, Yang W, Gai Xd, Jia T, Lei Ym, Li Y. Acta Histochem. 2013 Mar;115(2):151-7. Doi: 10.1016/J.Acthis.2012.06.002. Epub 2012 Jun 29. Foxp3 And Tlr4 Protein Expression Are Correlated In Non-Small Cell Lung Cancer: Implications For Tumor Pr...
Wang Yz, Tian Ff, Liu H, Zhang W, Li J, Xiao B, Zhou Wb. J Neuroimmunol. 2013 Apr 15;257(1-2):67-75. Doi: 10.1016/J.Jneuroim.2013.01.006. Epub 2013 Feb 12. Macrophage Migration Inhibitory Factor Is Necessary For The Lipo-Oligosaccharide-Induced Re...
Yang Ss, Tang L, Li Rg, Ge Gh, Qu Xk, Ma Jw, Qiao Zy, Zhang L, Liu Hj, Hou Ym, Cao H, Hao Zm, Cheng Wb, Wang Hw. Neuro Endocrinol Lett. 2014;35(1):80-6. The Effects Of Subclinical Hypothyroidism On Serum Lipid Level And Tlr4 Expression Of Monocyte...
Yang Ss, Li R, Qu X, Fang W, Quan Z. Cell Immunol. 2012 Sep;279(1):96-102. Doi: 10.1016/J.Cellimm.2012.09.008. Epub 2012 Oct 1. Atorvastatin Decreases Toll-Like Receptor 4 Expression And Downstream Signaling In Human Monocytic Leukemia Cells.
Liu X, Hu R, Lian H, Liu Y, Liu J, Liu J, Lin G, Liu L, Duan X, Yong Kt, Ye L. Int J Nanomedicine. 2015 May 20;10:3651-62. Doi: 10.2147/Ijn.S81395. Ecollection 2015. Dual-Color Immunofluorescent Labeling With Quantum Dots Of The Diabetes-Associate...
Xu M, Wang Kn, Wu K, Wang Xp. Gut Liver. 2015 May 23;9(3):411-6. Doi: 10.5009/Gnl14050. Pyrrolidine Dithiocarbamate Inhibits Nuclear Factor ??b And Toll-Like Receptor 4 Expression In Rats With Acute Necrotizing Pancreatitis.
Ou Jm, Zhang Xp, Wu Cj, Wu Dj, Yan P. J Zhejiang Univ Sci B. 2012 Nov;13(11):919-31. Doi: 10.1631/Jzus.B1100351. Effects Of Dexamethasone And Salvia Miltiorrhiza On Multiple Organs In Rats With Severe Acute Pancreatitis.
Cui L, Feng L, Zhang Zh, Jia Xb. Int Immunopharmacol. 2014 Nov;23(1):294-303. Doi: 10.1016/J.Intimp.2014.09.005. Epub 2014 Sep 18. The Anti-Inflammation Effect Of Baicalin On Experimental Colitis Through Inhibiting Tlr4/Nf-??b Pathway Activation.
Liu Mw, Wang Yh, Qian Cy, Li H. Int J Mol Med. 2014 Dec;34(6):1492-504. Doi: 10.3892/Ijmm.2014.1943. Epub 2014 Sep 23. Xuebijing Exerts Protective Effects On Lung Permeability Leakage And Lung Injury By Upregulating Toll-Interacting Protein Expres...
Li X, Qian D, Ju F, Wang B. Oncol Lett. 2015 Jan;9(1):365-370. Epub 2014 Oct 15. Upregulation Of Toll-Like Receptor 2 Expression In Colorectal Cancer Infected By Human Cytomegalovirus.
Yuan Z, Zhang J, Yang C. Evid Based Complement Alternat Med. 2014;2014:620359. Doi: 10.1155/2014/620359. Epub 2014 Aug 17. Ligusticum Wallichii Extract Inhibited The Expression Of Il-1?? After Ami In Rats.

Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at [email protected] for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
  • Q: What are some alternative names that could be used to describe this product?
    A: Some common names include but are not limited to tlr4 antibody, tlr 4 antibody, toll like receptor 4 antibody, toll-like receptor 4 antibody
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