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Product Info Summary
| SKU: | PA1484 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Toll-like receptor 4 TLR4 Antibody
SKU/Catalog Number
PA1484
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Toll-like receptor 4 TLR4 Antibody catalog # PA1484. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Toll-like receptor 4 TLR4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1484)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human TLR4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1484 is reactive to TLR4 in Human
Applications
PA1484 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
80 kDa
Calculated molecular weight
95680 MW
Background of TLR4
TLR4, Toll-like receptor 4, is a protein that in humans is encoded by the TLR4 gene. TLR 4 is a toll-like receptor. TLR4, the human homolog of Drosophila Toll, is a type I transmembrane protein with an extracellular domain consisting of a leucine-rich repeat region and an intracellular domain homologous to that of human interleukin-1 receptor. The TLR4 gene is mapped to chromosome 9q32-q33 by fluorescence in situ hybridization.It detects lipopolysaccharide from Gram-negative bacteria and is thus important in the activation of the innate immune system.The protein encoded by this gene is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Anti-TLR4 antibody, PA1484, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: SMMC Cell Lysate
Click image to see more details
Anti-TLR4 antibody, PA1484, IHC(P)
IHC(P): Human Lung Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For TLR4 (Source: Uniprot.org, NCBI)
Gene Name
TLR4
Full Name
Toll-like receptor 4
Weight
95680 MW
Superfamily
Toll-like receptor family
Alternative Names
76B357.1; ARMD10; CD284 antigen; CD284; hTollhomolog of Drosophila toll; tlr4 76B357.1; TLR4 facs; TLR4 flow cytometry; TLR4 human; TLR4 ihc; TLR4 western blot; TLR4; TOLL; toll-like receptor 4 TLR4 ARMD10, CD284, TLR-4, TOLL toll like receptor 4 toll-like receptor 4|hToll|homolog of Drosophila toll|toll like receptor 4 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TLR4, check out the TLR4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TLR4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Toll-like receptor 4 TLR4 Antibody (PA1484)
Hello CJ!
PA1484 has been cited in 30 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of curcumin supplementation on TLR4 mediated non-specific immune responses in liver of laying hens under high-temperature conditions
Liu J,Zhang N,Zhang M,Yin H,Zhang X,Wang X,Wang X,Zhao Y.N-acetylserotonin alleviated the expression of interleukin-1β in retinal ischemia-reperfusion rats via the TLR4/NF-κB/NLRP3 pathway.Exp Eye Res.2021 May 14:108595.doi:10.1016/j.exer.2021.108595.Epub ahead of print.PMID:34000276.
Species: Rat
PA1484 usage in article: APP:IHC, SAMPLE:RETINAL TISSUE, DILUTION:1:1000
Daiyang Xia,Lin Yang,Yu Li,Jianying Chen,Xiufen Zhang,Heng Wang,Shuangshuang Zhai,Xianzhi Jiang,Giuseppe Meca,Shunxiang Wang,Liang Huang,Shanshan Zhu,Yang Fu,Weiqing Ma,Yongwen Zhu,Hui Ye,Wence Wang,Melatonin alleviates Ochratoxin A-induced liver inflamma
Species: Cherry Valley duckling
PA1484 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA
Regulation on Toll-like Receptor 4 and Cell Barrier Function by Rab26 siRNA-loaded DNA Nanovector in Pulmonary Microvascular Endothelial Cells
Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4)-Mediated Signaling
Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response
Allicin Alleviates Reticuloendotheliosis Virus-Induced Immunosuppression via ERK/Mitogen-Activated Protein Kinase Pathway in Specific Pathogen-Free Chickens
Phospholipase A2 of Peroxiredoxin 6 Plays a Critical Role in Cerebral Ischemia/Reperfusion Inflammatory Injury
Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therapy Using the Bifidobacterium-Mediated Recombinant HSV-1 Thymidine Kinase and Ganciclovir
Knockdown of versicanV1 induces a severe inflammatory response in LPS-induced acute lung injury via the TLR2-NF-?B signaling pathway in C57BL/6J mice
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Customer Q&As
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5 Customer Q&As for Anti-Toll-like receptor 4 TLR4 Antibody
Question
We are currently using anti-TLR4 antibody PA1484 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
The anti-TLR4 antibody (PA1484) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-07
Question
Our lab used your anti-TLR4 antibody for IHC on cerebellum last year. I am using human, and We intend to use the antibody for WB next. My lab would like examining cerebellum as well as fetal liver in our next experiment. Could give a recommendation on which antibody would work the best for WB?
P. Zhao
Verified customer
Asked: 2019-12-30
Answer
I looked at the website and datasheets of our anti-TLR4 antibody and I see that PA1484 has been tested on human in both IHC and WB. Thus PA1484 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-30
Question
We have been able to see staining in human spleen. Any tips? Is anti-TLR4 antibody supposed to stain spleen positively?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
According to literature spleen does express TLR4. According to Uniprot.org, TLR4 is expressed in leukocyte, spleen, fetal liver, lung placenta, hippocampus, kidney uterus, cerebellum, monocyte, among other tissues. Regarding which tissues have TLR4 expression, here are a few articles citing expression in various tissues:
Cerebellum, Pubmed ID: 15489334
Fetal liver, Lung, and Placenta, Pubmed ID: 9435236
Hippocampus, Kidney, and Uterus, Pubmed ID: 14702039
Monocyte, Pubmed ID: 16622205
Spleen, Pubmed ID: 9237759
Boster Scientific Support
Answered: 2019-07-16
Question
My colleagues were happy with the WB result of your anti-TLR4 antibody. However we have seen positive staining in leukocyte cell membrane using this antibody. Is that expected? Could you tell me where is TLR4 supposed to be expressed?
K. Kulkarni
Verified customer
Asked: 2018-02-12
Answer
From literature, leukocyte does express TLR4. Generally TLR4 expresses in cell membrane. Regarding which tissues have TLR4 expression, here are a few articles citing expression in various tissues:
Cerebellum, Pubmed ID: 15489334
Fetal liver, Lung, and Placenta, Pubmed ID: 9435236
Hippocampus, Kidney, and Uterus, Pubmed ID: 14702039
Monocyte, Pubmed ID: 16622205
Spleen, Pubmed ID: 9237759
Boster Scientific Support
Answered: 2018-02-12
Question
My lab would like using your anti-TLR4 antibody for positive regulation of i-kappab kinase/nf-kappab signaling studies. Has this antibody been tested with western blotting on lung cancer tissue? We would like to see some validation images before ordering.
R. Krishna
Verified customer
Asked: 2016-10-04
Answer
I appreciate your inquiry. This PA1484 anti-TLR4 antibody is validated on hela cell lysate, smmc cell lysate, lung cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-10-04
