Click image to see more details
Product Info Summary
| SKU: | RP1000 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-TNF alpha Antibody Picoband®
SKU/Catalog Number
RP1000
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TNF alpha Antibody catalog # RP1000. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TNF alpha Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1000)
Host
Rabbit
Contents
Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse TNF alpha recombinant protein (Position: L80-L235).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1000 is reactive to Tnf in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
17 kDa
Calculated molecular weight
25896 MW
Background of TNF-alpha
TNF alpha (Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1000 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, By Heat
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
Click image to see more details
Figure. Western blot analysis of TNF alpha using anti-TNF alpha antibody (RP1000).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V(Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane: Recombinant Mouse TNFα Protein 0.5ng,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # RP1000) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 17KD. The expected band size for TNF alpha is at 17KD.
Protein Target Info & Infographic
Gene/Protein Information For Tnf (Source: Uniprot.org, NCBI)
Gene Name
Tnf
Full Name
Tumor necrosis factor
Weight
25896 MW
Superfamily
Tumor necrosis factor family
Alternative Names
Tumor necrosis factor;Cachectin;TNF-alpha;Tumor necrosis factor ligand superfamily member 2;TNF-a;Tumor necrosis factor, membrane form;N-terminal fragment;NTF;Intracellular domain 1;ICD1;Intracellular domain 2;ICD2;C-domain 1;C-domain 2;Tumor necrosis factor, soluble form;Tnf;Tnfa, Tnfsf2; TNF DIF-alpha, TNFA, TNFSF2, TNLG1F, TNF tumor necrosis factor tumor necrosis factor|APC1 protein|TNF, macrophage-derived|TNF, monocyte-derived|TNF-a|tumor necrosis factor ligand 1F|tumor necrosis factor ligand superfamily member 2|tumor necrosis factor-alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tnf, check out the Tnf Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tnf: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TNF alpha Antibody Picoband® (RP1000)
Hello CJ!
RP1000 has been cited in 63 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Lan Q,Lu R,Chen H,Pang Y, Xiong F,Shen C,Qin Z,Zheng L,Xu G,Zhao J.MMP-13 enzyme and pH responsive theranostic nanoplatform for osteoarthritis.J Nanobiotechnology.2020 Aug 27;18(1):117.doi:10.1186/s12951-020-00666-7. PMID:32854712;PMCID:PMC7450974.
Species: Mouse
RP1000 usage in article: APP:IF, SAMPLE:CHONDROCYTES, DILUTION:1:100
Resveratrol attenuates hyperoxia%u2010induced oxidative stress, inflammation and fibrosis and suppresses Wnt/%u03B2%u2010catenin signalling in lungs of neonatal rats
Efficacy of acetylshikonin in preventing obesity and hepatic steatosis in db/db mice
Protective effects of baicalin on carbon tetrachloride induced liver injury by activating PPAR%u03B3 and inhibiting TGF%u03B21
Immunomodulatory properties of quercetin-3-O-%u03B1-L-rhamnopyranoside from Rapanea melanophloeos against influenza a virus
Neuroprotective effects of %u03B1-lipoic acid on long-term experimental autoimmune encephalomyelitis.
Polymeric micelles for potentiated antiulcer and anticancer activities of naringin
Rhein inhibits lipopolysaccharide-induced intestinal injury during sepsis by blocking the toll-like receptor 4 nuclear factor-%u03BAB pathway
Effects of aqueous extracts of Taraxacum Officinale on expression of tumor necrosis factor-alpha and intracellular adhesion molecule 1 in LPS-stimulated %u2026
Characterization of acetaminophen-induced cytotoxicity in target tissues
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Customer Q&As
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21 Customer Q&As for Anti-TNF alpha Antibody Picoband®
Question
I have attached the WB image, lot number and protocol we used for prostatic carcinoma using anti-TNF alpha antibody RP1000. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-10
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for prostatic carcinoma using anti-TNF alpha antibody RP1000. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-24
Question
My question regarding product RP1000, anti-TNF alpha antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Jackson
Verified customer
Asked: 2020-02-14
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1000 anti-TNF alpha antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-14
Question
Do you have a BSA free version of anti-TNF alpha antibody RP1000 available?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TNF alpha antibody RP1000 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-30
Question
We have seen staining in mouse prostatic carcinoma. Any tips? Is anti-TNF alpha antibody supposed to stain prostatic carcinoma positively?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
From literature prostatic carcinoma does express TNF. From Uniprot.org, TNF is expressed in leukocyte, blood, prostatic carcinoma, among other tissues. Regarding which tissues have TNF expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Prostatic carcinoma, Pubmed ID: 8597870, 10205166
Boster Scientific Support
Answered: 2020-01-29
Question
you antibody to test anti-TNF alpha antibody RP1000 on mouse prostatic carcinoma for research purposes, then I may be interested in using anti-TNF alpha antibody RP1000 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
The products we sell, including anti-TNF alpha antibody RP1000, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-01
Question
Our lab were well pleased with the WB result of your anti-TNF alpha antibody. However we have been able to see positive staining in leukocyte cell membrane using this antibody. Is that expected? Could you tell me where is TNF supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
Based on literature, leukocyte does express TNF. Generally TNF expresses in cell membrane. Regarding which tissues have TNF expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Prostatic carcinoma, Pubmed ID: 8597870, 10205166
Boster Scientific Support
Answered: 2019-11-18
Question
Is a blocking peptide available for product anti-TNF alpha antibody (RP1000)?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
We do provide the blocking peptide for product anti-TNF alpha antibody (RP1000). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-08
Question
Is this RP1000 anti-TNF alpha antibody reactive to the isotypes of TNF?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
The immunogen of RP1000 anti-TNF alpha antibody is E. coli-derived mouse TNF alpha recombinant protein(Position: L80-L235). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-27
Question
We had a look in your products and it seems that RP1000 Anti-TNF alpha Antibody is the most suitable for our research. Would you be able to please give us the epitopes of this antibody?
Verified Customer
Verified customer
Asked: 2019-05-09
Answer
I am sorry to tell you that there is no available information about the epitope recognized by RP1000 Anti-TNF alpha Antibody, as it has not been mapped yet.
Boster Scientific Support
Answered: 2019-05-09
Question
I would be interested in your anti TNF antibody (RP1000). Does it recognize both the cleaved and full length forms of TNF or only the cleaved one? Does it cross-react with human? I need an antibody that does NOT recognize the human TNF.
Verified Customer
Verified customer
Asked: 2019-04-17
Answer
We didn't obtain the target band by working on tissue or cell samples. We tested the recombinant protein and didn't know if RP1000 recognized the cleaved one or full length forms of TNF. We have another anti-mouse TNF alpha antibody A00002-4 which recognize full length forms of TNF. https://www.bosterbio.com/anti-tnf-alpha-picoband-trade-antibody-a00002-4-boster.html?options=cart A00002-4 doesn't cross react with human.
Boster Scientific Support
Answered: 2019-04-17
Question
Will anti-TNF alpha antibody RP1000 work for WB with prostatic carcinoma?
Verified Customer
Verified customer
Asked: 2019-03-11
Answer
According to the expression profile of prostatic carcinoma, TNF is highly expressed in prostatic carcinoma. So, it is likely that anti-TNF alpha antibody RP1000 will work for WB with prostatic carcinoma.
Boster Scientific Support
Answered: 2019-03-11
Question
Would anti-TNF alpha antibody RP1000 work on dog ELISA with blood?
Verified Customer
Verified customer
Asked: 2019-01-04
Answer
Our lab technicians have not validated anti-TNF alpha antibody RP1000 on dog. You can run a BLAST between dog and the immunogen sequence of anti-TNF alpha antibody RP1000 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog blood in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-04
Question
To Boster Technical Support; I am looking for antibodies against neutrophils and TNF-alpha for Immunohistochemistry. I came across in your product RP1000 Anti-TNF alpha Antibody that can be used for staining. But, I have few questions. Do these antibodies work on tissue sections of knee joint? Knee joints are 4% formaldehyde fixed and paraffin embedded sections. Before embedding, knee joints are decalcified with 20% formic acid + Sodium citrate for 3 days. Do these antibodies work on this particular treated sections?
Verified Customer
Verified customer
Asked: 2018-04-27
Answer
Thank you for your inquiry, unfortunately, we were not able to test every organ or tissue when we establish the product. However, if the knee joints were properly decalcified and the species is tested, we don't see any problems why these antibodies should not work in these sections.
Boster Scientific Support
Answered: 2018-04-27
Question
We are currently using anti-TNF alpha antibody RP1000 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-26
Answer
The anti-TNF alpha antibody (RP1000) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-26
Question
I need help, I already received the products RP1000 Anti-TNF alpha Antibody this week and I run to detect the TNF-alpha that is supposed to be overexpressed in some transfected cells, but the results in WB I got strange band around 40 KD and no bands at the position of 17 KD.
Verified Customer
Verified customer
Asked: 2017-08-28
Answer
The TNF-alpha is known to form a homotrimer, the expected molecular weight for that band would be around 58 kDa. In order for us to help you more about the nature of the band you are observing, it would be helpful if you could provide us some additional details about your protocol. Including; what type of cells were you staining? What species was the transfected TNF alpha? Was the transfected protein tagged? How were the cells lysed? How much protein was loaded per lane, and were the samples reduced and denatured? What blocking agent you used? What was the primary antibody dilution and incubation time? What secondary antibody was used? What was the dilution and incubation time, and has this secondary antibody been working well with other primary antibodies?
Boster Scientific Support
Answered: 2017-08-28
Question
Does RP1000 anti-TNF alpha antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-05-17
Answer
It shows on the product datasheet, RP1000 anti-TNF alpha antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-05-17
Question
I see that the anti-TNF alpha antibody RP1000 works with WB, what is the protocol used to produce the result images on the product page?
H. Johnson
Verified customer
Asked: 2017-01-31
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-01-31
Question
Our lab used your anti-TNF alpha antibody for ELISA on blood a few months ago. I am using mouse, and I plan to use the antibody for WB next. We are interested in examining blood as well as prostatic carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
D. Jones
Verified customer
Asked: 2016-10-04
Answer
I looked at the website and datasheets of our anti-TNF alpha antibody and I see that RP1000 has been tested on mouse in both ELISA and WB. Thus RP1000 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-10-04
Question
I was wanting to use your anti-TNF alpha antibody for WB for mouse prostatic carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse prostatic carcinoma identification?
D. Parker
Verified customer
Asked: 2015-10-22
Answer
You can see on the product datasheet, RP1000 anti-TNF alpha antibody has been tested for ELISA, IHC, WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse prostatic carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-10-22
Question
I would like to investigate TNF-alpha expression rat perivascular adipose tissue using immunohistochemistry (paraffin sections) and western blotting. Do you know if your product RP1000 Anti-TNF alpha Antibody would be suitable to determine expression in rat PVAT or any other white adipose tissue?
Verified Customer
Verified customer
Asked: 2013-06-03
Answer
Yes, I am glad to say that our products RP1000 Anti-TNF alpha Antibody is tested and guaranteed for rat in WB and IHC-P. However, in regards to the perivascular adipose tissues, we do not have any records about it yet, so we suggest running a positive control with a tissue that is known for its expression of TNF alpha
Boster Scientific Support
Answered: 2013-06-03
