Human Kallistatin/Serpina4 PicoKine® Quick ELISA Kit

Product Name

Human Kallistatin/Serpina4 PicoKine® Quick ELISA Kit

See all Serpin A4/Kallistatin products

SKU/Catalog Number

FEK0836

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Kallistatin/Serpina4 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human SERPINA4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Cite This Product

Human Kallistatin/Serpina4 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0836)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: Q21-P427

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

See datasheet of FEK0836 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0836 is reactive to SERPINA4 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Application Guarantee

FEK0836 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Serpin A4/Kallistatin

Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4, also called Kallistatin, is a protein that in humans is encoded by the SERPINA4 gene. This gene is mapped to chromosome 14q32.13. Kallistatin is a serine proteinase inhibitor (serpin) and a heparin-binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function. It shows that kallistatin plays a role in neointima hyperplasia. The potential role of kallistatin was investigated in angiogenesis in vitro and in vivo, and presented results demonstrating a novel role of the protein in the inhibition of angiogenesis and tumor growth.

Kit Components

Catalog number	Description	Quantity	Volume
FEK0836-CAP	Anti-tag Pre-coated 96-well Strip Microplate	1	12 strips of 8 wells
EK0836-ST	Human Serpin A4/Kallistatin Standard	2	10 ng/tube
FEK0836-A	Human SERPINA4 Antibody A	1	3ml
FEK0836-B	Human SERPINA4 Antibody B	1	3ml
AR1106-1	Sample Diluent	1	15ml
AR1106-7	TBS-T Wash Buffer (25x)	1	12ml
AR1104	Color Developing Reagent (TMB)	1	10ml
AR1105	Stop Solution	1	10ml
PLA-SEA	Adhesive Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional)
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.
• Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.010	0.132	0.225	0.431	0.753	1.438	2.208	2.404

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Kallistatin/Serpina4 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Serpin A4/Kallistatin in ELISA kits from four different production batches/lots.

Gene/Protein Information For SERPINA4 (Source: Uniprot.Org, NCBI)

Alternative Names

antitrypsin), member 4; KAL; Kallistatin; KLST; KSTKallikrein inhibitor; member 4; PI-4; PI4Peptidase inhibitor 4; protease inhibitor 4 (kallistatin); serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; Serpin A4; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin) SERPINA4 KAL, KLST, KST, PI-4, PI4, kallistatin serpin family A member 4 kallistatin|kallikrein inhibitor|peptidase inhibitor 4|protease inhibitor 4 (kallistatin)|serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4|serpin A4|serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4|sserpin family A member 4|testicular secretory protein Li 22

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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