Click image to see more details
-
-
-
-
-
+1
Product Info Summary
| SKU: | A01096-4 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Thymic stromal lymphopoietin TSLP Antibody
SKU/Catalog Number
A01096-4
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Thymic stromal lymphopoietin TSLP Antibody (Catalog # A01096-4). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Mouse, Rat.
Storage & Handling
TSLP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Thymic stromal lymphopoietin TSLP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01096-4)
Host
Rabbit
Contents
TSLP Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-TSLP antibody was raised against a peptide corresponding to 20 amino acids near the center of mouse TSLP. The immunogen is located within the amino acids 70-120 of TSLP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of SCARB1 are known to exist; this antibody can detect both isoforms.
Reactive Species
A01096-4 is reactive to Tslp in Mouse, Rat
Applications
A01096-4 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
18141 MW
Background of Tslp
Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. TSLP is a four-helix-bundle cytokine that is expressed mainly by barrier epithelial cells and is a potent activator of several cell types such as myeloid dendritic cells. TSLP is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Amino acid sequence analysis has shown poor homology between human and mouse TSLP although they exhibit similar biological functions and are expressed in similar tissues. At least two differentially spliced isoforms of TSLP are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.5 - 2 μg/mL; IHC-P: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western Blot Validation in Mouse A-20 Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: TSLP A01096-4 (A: 0.5 μg/mL, B: 1 μg/mL, C: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Immunofluorescence Validation of TSLP in Mouse Brain Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed Mouse Brain Tissue labeling TSLP with A01096-4 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Click image to see more details
Immunohistochemistry Validation of TSLP in Mouse Brain Tissue
Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using anti-TSLP antibody (A01096-4) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Click image to see more details
Induction Validation of TSLP in Nasal Mucosa of Allergic Rhinitis (AR) mice (Wang et al.
Click image to see more details
Induction Validation of TSLP in Rat VSMCs (Zhao et al.
Protein Target Info & Infographic
Gene/Protein Information For Tslp (Source: Uniprot.org, NCBI)
Gene Name
Tslp
Full Name
Thymic stromal lymphopoietin
Weight
18141 MW
Alternative Names
thymic stromal lymphopoietin; TSLP Tslp|thymic stromal lymphopoietin|thymic stromal lymphopoietin|thymic stroma-derived lymphopoietin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tslp, check out the Tslp Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tslp: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Thymic stromal lymphopoietin TSLP Antibody (A01096-4)
Hello CJ!
No publications found for A01096-4
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Thymic stromal lymphopoietin TSLP Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Thymic stromal lymphopoietin TSLP Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
