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Product Info Summary
| SKU: | A01096 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-TSLP Antibody Picoband®
SKU/Catalog Number
A01096
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TSLP Antibody Picoband® catalog # A01096. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TSLP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01096)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TSLP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01096 is reactive to TSLP in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
18 kDa
Calculated molecular weight
16.152kDa
Background of Tslp
Thymic stromal lymphopoietin, also called TSLP is a protein belonging to the cytokine family. This gene is mapped to 5q22.1. It encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c (+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01096 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml
Positive Control
WB: human Hela whole cell, mouse spleen tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TSLP using anti-TSLP antibody (A01096).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: mouse spleen tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSLP antigen affinity purified polyclonal antibody (Catalog # A01096) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSLP at approximately 18KD. The expected band size for TSLP is at 18KD.
Protein Target Info & Infographic
Gene/Protein Information For TSLP (Source: Uniprot.org, NCBI)
Gene Name
TSLP
Full Name
Thymic stromal lymphopoietin
Weight
16.152kDa
Alternative Names
Thymic stromal lymphopoietin; TSLP; Tslp|thymic stromal lymphopoietin|thymic stromal lymphopoietin|thymic stroma-derived lymphopoietin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TSLP, check out the TSLP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TSLP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TSLP Antibody Picoband® (A01096)
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No publications found for A01096
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Customer Q&As
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4 Customer Q&As for Anti-TSLP Antibody Picoband®
Question
We are currently using anti-TSLP antibody A01096 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
The anti-TSLP antibody (A01096) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-20
Question
We have seen staining in mouse brain. Any tips? Is anti-TSLP antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
According to literature brain does express TSLP. According to Uniprot.org, TSLP is expressed in epithelial cell of pancreas, brain, lung testis, among other tissues. Regarding which tissues have TSLP expression, here are a few articles citing expression in various tissues:
Brain, Lung, and Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-12-04
Question
My colleagues were content with the WB result of your anti-TSLP antibody. However we have been able to see positive staining in epithelial cell of pancreas secreted. using this antibody. Is that expected? Could you tell me where is TSLP supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
Based on literature, epithelial cell of pancreas does express TSLP. Generally TSLP expresses in secreted. Regarding which tissues have TSLP expression, here are a few articles citing expression in various tissues:
Brain, Lung, and Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-09-17
Question
We bought anti-TSLP antibody for WB on lung testis last year. I am using human, and I plan to use the antibody for ELISA next. Our lab want to know about examining lung testis as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
D. Patel
Verified customer
Asked: 2017-04-13
Answer
I viewed the website and datasheets of our anti-TSLP antibody and it seems that A01096 has been validated on human in both WB and ELISA. Thus A01096 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-04-13
