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Product Info Summary
| SKU: | EK0990 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. |
Product info
Product Name
Human TNFSF14/LIGHT ELISA Kit PicoKine®
View all LIGHT/TNFSF14 ELISA kits
SKU/Catalog Number
EK0990
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TNFSF14/LIGHT ELISA Kit PicoKine® (96 Tests). Quantitate Human TNFSF14 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TNFSF14/LIGHT ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0990)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: D74-V240
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0990 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0990 is reactive to TNFSF14 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.
Application Guarantee
EK0990 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of LIGHT/TNFSF14
Tumor necrosis factor ligand superfamily member 14 is a protein that in humans is encoded by the TNFSF14 gene. TNFSF14 has also been designated as CD258, as well as LIGHT. It was mapped on chromosome 19p13.3. The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0990-CAP | Anti-Human TNFSF14 Pre-coated 96-well strip microplate | 1 |
| EK0990-ST | Human TNFSF14 Standard | 2 vials, 10 ng/tube |
| EK0990-DA | Human TNFSF14 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.006 | 0.086 | 0.146 | 0.313 | 0.533 | 0.993 | 1.745 | 2.284 |
Data Example Images
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Human TNFSF14/LIGHT PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect LIGHT/TNFSF14, Dilution ratio of 1:1, concentration in serum and plasma is 0-259 pg/ml..
Some articles we found to cite concentrations of LIGHT/TNFSF14 in samples: 19380287 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TNFSF14/LIGHT ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 71 | 260 | 754 | 65 | 241 | 814 |
| Standard deviation | 3.9 | 14.04 | 36.94 | 4.81 | 13.97 | 39.88 |
| CV (%) | 5.5% | 5.4% | 4.9% | 7.4% | 5.8% | 4.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of LIGHT/TNFSF14 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 71 | 75 | 65 | 70 | 70 | 3.56 | 5% |
| Sample 2 | 260 | 268 | 247 | 270 | 261 | 9.03 | 3.4% |
| Sample 3 | 754 | 758 | 794 | 885 | 797 | 52.72 | 6.6% |
Protein Target Info & Infographic
Gene/Protein Information For TNFSF14 (Source: Uniprot.Org, NCBI)
Gene Name
TNFSF14
Full Name
Tumor necrosis factor ligand superfamily member 14
Weight
26350 MW
Superfamily
tumor necrosis factor family
Alternative Names
Tumor necrosis factor ligand superfamily member 14;Herpes virus entry mediator ligand;HVEM-L;Herpesvirus entry mediator ligand;CD258;Tumor necrosis factor ligand superfamily member 14, membrane form;Tumor necrosis factor ligand superfamily member 14, soluble form;TNFSF14;HVEML, LIGHT;UNQ391/PRO726; TNFSF14 CD258, HVEML, LIGHT, LTg TNF superfamily member 14 tumor necrosis factor ligand superfamily member 14|herpesvirus entry mediator ligand|tumor necrosis factor (ligand) superfamily, member 14|tumor necrosis factor ligand 1D|tumor necrosis factor superfamily member 14
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TNFSF14, check out the TNFSF14 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNFSF14: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human TNFSF14/LIGHT ELISA Kit PicoKine® (EK0990)
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No publications found for EK0990
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Human TNFSF14/LIGHT ELISA Kit PicoKine®
Question
Do you know if TNFSF14 can be measured in mother’s milk sample using the EK0990 kit?
Verified customer
Asked: 2019-10-08
Answer
The Human TNFSF14/LIGHT ELISA Kit PicoKine EK0990 has been tested and validated for cell culture supernates, serum, plasma(heparin, EDTA) and saliva. We have not tested EK0990 using mother’s milk samples. In theory, the ELISA kit will work for all sample types if the target protein is present at a level that falls within the linear range of the ELISA kit detection range. We guarantee the kit to work on the sample types that we have tested. For sample types that we have not tested for, we suggest you run pilot experiments to decide the optimal sample dilution.
Boster Scientific Support
Answered: 2019-10-09
Question
I am interested to purchase your product Human TNFSF14/LIGHT ELISA Kit and use it in plasma sample. But I am wondering if the EDTA or Heparin in the plasma would affect the results. Since it is necessary for me to use EDTA in my samples. Please let me know. Thank you
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
The product Human TNFSF14/LIGHT ELISA Kit has been quality tested with EDTA, heparin and citrate anti-coagulants, so no problem to use these antibody in your plasma sample with EDTA.
Boster Scientific Support
Answered: 2019-09-16
Question
Q: how to analyze ELISA data? I have performed LIGHT level in serum.
Verified Customer
Verified customer
Asked: 2019-08-17
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-08-17
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-12-15
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-12-15
Question
Q: Can LIGHT ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
T. Wright
Verified customer
Asked: 2018-08-11
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-08-11
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification LIGHT using the EK0990 Human LIGHT Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-06-01
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2018-06-01
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-03-29
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-03-29
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-02-17
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-02-17
Question
Q: What is the optimal O.D. value for LIGHT ELISA kit? I performed your LIGHT ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain LIGHT even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-01-16
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this LIGHT ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of LIGHT that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-01-16
Question
Q: how to thaw whole blood sample for LIGHT ELISA after freezing?
Verified Customer
Verified customer
Asked: 2017-12-18
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test LIGHT for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2017-12-18
