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Product Info Summary
| SKU: | A02952-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-TACI/TNFRSF13B Antibody
View all TACI/TNFRSF13B/CVID Antibodies
SKU/Catalog Number
A02952-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TACI/TNFRSF13B Antibody catalog # A02952-1. Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TACI/TNFRSF13B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02952-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human TACI recombinant protein (Position: M1-S165).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02952-1 is reactive to TNFRSF13B in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
40 kDa
Calculated molecular weight
79686 MW
Background of TACI/TNFRSF13B/CVID
Transmembrane activator and CAML interactor (TACI), also known as tumor necrosis factor receptor superfamily member 13B (TNFRSF13B) is a protein that in humans is encoded by the TNFRSF13B gene. The protein encoded by this gene is a lymphocyte-specific member of the tumor necrosis factor (TNF) receptor superfamily. It interacts with calcium-modulator and cyclophilin ligand (CAML). The protein induces activation of the transcription factors NFAT, AP1, and NF-kappa-B and plays a crucial role in humoral immunity by interacting with a TNF ligand. This gene is located within the Smith-Magenis syndrome region on chromosome 17.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02952-1 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 2-5 μg/ml
Positive Control
IHC: human breast cancer tissue, human cervical cancer tissue, human colorectal adenocarcinoma tissue, human spleen tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of TACI using anti-TACI antibody (A02952-1).
TACI was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TACI Antibody (A02952-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 2. IHC analysis of TACI using anti-TACI antibody (A02952-1).
TACI was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TACI Antibody (A02952-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of TACI using anti-TACI antibody (A02952-1).
TACI was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TACI Antibody (A02952-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of TACI using anti-TACI antibody (A02952-1).
TACI was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TACI Antibody (A02952-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For TNFRSF13B (Source: Uniprot.org, NCBI)
Gene Name
TNFRSF13B
Full Name
Tumor necrosis factor receptor superfamily member 13B
Weight
79686 MW
Alternative Names
Tumor necrosis factor receptor superfamily member 13B; Transmembrane activator and CAML interactor; CD267; TNFRSF13B; TACI TNFRSF13B CD267, CVID, CVID2, IGAD2, RYZN, TACI, TNFRSF14B TNF receptor superfamily member 13B tumor necrosis factor receptor superfamily member 13B|transmembrane activator and CAML interactor|tumor necrosis factor receptor 13B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNFRSF13B, check out the TNFRSF13B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNFRSF13B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TACI/TNFRSF13B Antibody (A02952-1)
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Customer Q&As
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1 Customer Q&As for Anti-TACI/TNFRSF13B Antibody
Question
We are currently using anti-TACI/TNFRSF13B antibody A02952-1 for human tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
B. Li
Verified customer
Asked: 2017-03-28
Answer
The anti-TACI/TNFRSF13B antibody (A02952-1) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-03-28
