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Product Info Summary
| SKU: | A00291-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-ICOS Antibody Picoband®
SKU/Catalog Number
A00291-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ICOS Antibody Picoband® catalog # A00291-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ICOS Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00291-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse ICOS recombinant protein (Position: E21-S200).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00291-2 is reactive to Icos in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
20 kDa
Calculated molecular weight
22.625kDa
Background of ICOS
Inducible T-cell costimulator is an immune checkpoint protein that in humans is encoded by the ICOS gene. The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00291-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: mouse stomach tissue, mouse spleen tissue, mouse thymus tissue, mouse small intestine tissue, rat thymus tissue, human placenta tissue
IHC: mouse spleen tissue, mouse thymus tissue, rat spleen tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ICOS using anti-ICOS antibody (A00291-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse stomach tissue lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: mouse thymus tissue lysates,
Lane 4: mouse small intestine tissue lysates,
Lane 5: rat thymus tissue lysates,
Lane 6: human placenta tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICOS antigen affinity purified polyclonal antibody (Catalog # A00291-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICOS at approximately 20KD. The expected band size for ICOS is at 22KD.
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Figure 2. IHC analysis of ICOS using anti-ICOS antibody (A00291-2).
ICOS was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ICOS Antibody (A00291-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of ICOS using anti-ICOS antibody (A00291-2).
ICOS was detected in paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ICOS Antibody (A00291-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of ICOS using anti-ICOS antibody (A00291-2).
ICOS was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ICOS Antibody (A00291-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Icos (Source: Uniprot.org, NCBI)
Gene Name
Icos
Full Name
Inducible T-cell costimulator
Weight
22.625kDa
Alternative Names
Inducible T-cell costimulator; Activation-inducible lymphocyte immunomediatory molecule; CD28 and CTLA-4-like protein; CCLP; CD28-related protein 1; CRP-1; CD278; Icos; Ailim ICOS AILIM, CD278, CVID1 inducible T cell costimulator inducible T-cell costimulator|activation-inducible lymphocyte immunomediatory molecule|inducible T-cell co-stimulator|inducible costimulator
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Icos, check out the Icos Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Icos: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ICOS Antibody Picoband® (A00291-2)
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No publications found for A00291-2
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Customer Q&As
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17 Customer Q&As for Anti-ICOS Antibody Picoband®
Question
My team were well pleased with the WB result of your anti-ICOS antibody. However we have observed positive staining in leukocyte isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is ICOS supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
Based on literature, leukocyte does express ICOS. Generally ICOS expresses in isoform 1: cell membrane. Regarding which tissues have ICOS expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 11006126, 15489334
Thymus, Pubmed ID: 10779774
Boster Scientific Support
Answered: 2020-01-31
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thymus using anti-ICOS antibody A00291-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-06
Question
you antibody to test anti-ICOS antibody A00291-2 on rat thymus for research purposes, then I may be interested in using anti-ICOS antibody A00291-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
The products we sell, including anti-ICOS antibody A00291-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-12
Question
Is this A00291-2 anti-ICOS antibody reactive to the isotypes of ICOS?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
The immunogen of A00291-2 anti-ICOS antibody is E. coli-derived mouse ICOS recombinant protein (Position: E21-S200). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-28
Question
I am looking for using your anti-ICOS antibody for r-mmu-1257604; pip3 activates akt signaling studies. Has this antibody been tested with western blotting on rat thymus tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
Thank you for your inquiry. This A00291-2 anti-ICOS antibody is validated on mouse thymus tissue, stomach tissue, spleen tissue, small intestine tissue, rat thymus tissue, human placenta tissue. It is guaranteed to work for ELISA, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-05
Question
Can you help my question with product A00291-2, anti-ICOS antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00291-2 anti-ICOS antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-10
Question
Do you have a BSA free version of anti-ICOS antibody A00291-2 available?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ICOS antibody A00291-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-17
Question
Does anti-ICOS antibody A00291-2 work for WB with thymus?
N. Anderson
Verified customer
Asked: 2019-05-10
Answer
According to the expression profile of thymus, ICOS is highly expressed in thymus. So, it is likely that anti-ICOS antibody A00291-2 will work for WB with thymus.
Boster Scientific Support
Answered: 2019-05-10
Question
I was wanting to use your anti-ICOS antibody for WB for rat thymus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat thymus identification?
Verified Customer
Verified customer
Asked: 2018-11-02
Answer
You can see on the product datasheet, A00291-2 anti-ICOS antibody has been validated for ELISA, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat thymus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-02
Question
Please see the WB image, lot number and protocol we used for thymus using anti-ICOS antibody A00291-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-05-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-03
Question
We ordered your anti-ICOS antibody for ELISA on blood a few years ago. I am using mouse, and We intend to use the antibody for IHC next. We want examining blood as well as thymus in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2018-04-24
Answer
I took a look at the website and datasheets of our anti-ICOS antibody and it appears that A00291-2 has been validated on mouse in both ELISA and IHC. Thus A00291-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-04-24
Question
Would A00291-2 anti-ICOS antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-03-20
Answer
You can see on the product datasheet, A00291-2 anti-ICOS antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-03-20
Question
Is a blocking peptide available for product anti-ICOS antibody (A00291-2)?
Verified Customer
Verified customer
Asked: 2018-01-12
Answer
We do provide the blocking peptide for product anti-ICOS antibody (A00291-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-01-12
Question
We have observed staining in mouse blood. What should we do? Is anti-ICOS antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2017-07-19
Answer
According to literature blood does express ICOS. According to Uniprot.org, ICOS is expressed in leukocyte, blood, thymus, among other tissues. Regarding which tissues have ICOS expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 11006126, 15489334
Thymus, Pubmed ID: 10779774
Boster Scientific Support
Answered: 2017-07-19
Question
We are currently using anti-ICOS antibody A00291-2 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-27
Answer
The anti-ICOS antibody (A00291-2) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-27
Question
I see that the anti-ICOS antibody A00291-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-06-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-06-19
Question
Hello Boster Support: I am planning to purchase your product Anti-ICOS Antibody Picoband, so I want to ask which antigen retrieval do you recommend for it?
Verified Customer
Verified customer
Asked: 2017-01-30
Answer
The product Anti-ICOS Antibody Picoband antigen retrieval protocol used when testing in IHC-P are; fully immerse the slide in Tris EDTA buffer pH 9.0, cooked for 10-15 min with medium-low heat, then cook again for another 10-15 mins. Turn off for 10 mins but avoid drying out. Cool down at room temperature and washed with PBS buffer pH 7.4 for about 3-5mins.
Boster Scientific Support
Answered: 2017-01-30
