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Product Info Summary
| SKU: | A00291-Dyl550 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human ICOS DyLight® 550 conjugated Antibody
SKU/Catalog Number
A00291-Dyl550
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human ICOS DyLight® 550 conjugated Antibody catalog # A00291-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human ICOS DyLight® 550 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00291-Dyl550)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human ICOS recombinant protein (Position: E21-K140).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00291-Dyl550 is reactive to ICOS in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
22.625kDa
Background of ICOS
Inducible T-cell costimulator is an immune checkpoint protein that in humans is encoded by the ICOS gene. The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00291-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Validation Images & Assay Conditions
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Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For ICOS (Source: Uniprot.org, NCBI)
Gene Name
ICOS
Full Name
Inducible T-cell costimulator
Weight
22.625kDa
Alternative Names
Inducible T-cell costimulator; Activation-inducible lymphocyte immunomediatory molecule; CD278; ICOS; AILIM ICOS AILIM, CD278, CVID1 inducible T cell costimulator inducible T-cell costimulator|activation-inducible lymphocyte immunomediatory molecule|inducible T-cell co-stimulator|inducible costimulator
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ICOS, check out the ICOS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ICOS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human ICOS DyLight® 550 conjugated Antibody (A00291-Dyl550)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Human ICOS DyLight® 550 conjugated Antibody
Question
Our lab want to know about to test anti-Human ICOS DyLight® 550 conjugated antibody A00291-Dyl550 on human leukocyte for research purposes, then I may be interested in using anti-Human ICOS DyLight® 550 conjugated antibody A00291-Dyl550 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
The products we sell, including anti-Human ICOS DyLight® 550 conjugated antibody A00291-Dyl550, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-13
Question
Is there a BSA free version of anti-Human ICOS DyLight® 550 conjugated antibody A00291-Dyl550 available?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human ICOS DyLight® 550 conjugated antibody A00291-Dyl550 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-23
Question
We are currently using anti-Human ICOS DyLight® 550 conjugated antibody A00291-Dyl550 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
The anti-Human ICOS DyLight® 550 conjugated antibody (A00291-Dyl550) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-24
Question
Will anti-Human ICOS DyLight® 550 conjugated antibody A00291-Dyl550 work on canine Flow Cytometry with leukocyte?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
Our lab technicians have not tested anti-Human ICOS DyLight® 550 conjugated antibody A00291-Dyl550 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Human ICOS DyLight® 550 conjugated antibody A00291-Dyl550 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine leukocyte in Flow Cytometry, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-21
Question
Please see the WB image, lot number and protocol we used for leukocyte using anti-Human ICOS DyLight® 550 conjugated antibody A00291-Dyl550. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-09
Question
Does A00291-Dyl550 anti-Human ICOS DyLight® 550 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
You can see on the product datasheet, A00291-Dyl550 anti-Human ICOS DyLight® 550 conjugated antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-31
Question
Would anti-Human ICOS DyLight® 550 conjugated antibody A00291-Dyl550 work for Flow Cytometry with leukocyte?
M. Kulkarni
Verified customer
Asked: 2019-07-19
Answer
According to the expression profile of leukocyte, ICOS is highly expressed in leukocyte. So, it is likely that anti-Human ICOS DyLight® 550 conjugated antibody A00291-Dyl550 will work for Flow Cytometry with leukocyte.
Boster Scientific Support
Answered: 2019-07-19
Question
Is a blocking peptide available for product anti-Human ICOS DyLight® 550 conjugated antibody (A00291-Dyl550)?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
We do provide the blocking peptide for product anti-Human ICOS DyLight® 550 conjugated antibody (A00291-Dyl550). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-18
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-Human ICOS DyLight® 550 conjugated antibody A00291-Dyl550. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-29
Question
My question regarding product A00291-Dyl550, anti-Human ICOS DyLight® 550 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Brown
Verified customer
Asked: 2019-05-13
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00291-Dyl550 anti-Human ICOS DyLight® 550 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-13
Question
I was wanting to use your anti-Human ICOS DyLight® 550 conjugated antibody for Flow Cytometry for human leukocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukocyte identification?
Verified Customer
Verified customer
Asked: 2019-01-22
Answer
You can see on the product datasheet, A00291-Dyl550 anti-Human ICOS DyLight® 550 conjugated antibody has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-22
Question
Is this A00291-Dyl550 anti-Human ICOS DyLight® 550 conjugated antibody reactive to the isotypes of ICOS?
Verified Customer
Verified customer
Asked: 2018-09-07
Answer
The immunogen of A00291-Dyl550 anti-Human ICOS DyLight® 550 conjugated antibody is E. coli-derived human ICOS recombinant protein (Position: E21-K140). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-09-07
Question
We have seen staining in human blood. Are there any suggestions? Is anti-Human ICOS DyLight® 550 conjugated antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2018-02-02
Answer
Based on literature blood does express ICOS. Based on Uniprot.org, ICOS is expressed in leukocyte, blood, thymus, among other tissues. Regarding which tissues have ICOS expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 11006126, 15489334
Thymus, Pubmed ID: 10779774
Boster Scientific Support
Answered: 2018-02-02
Question
My boss were satisfied with the WB result of your anti-Human ICOS DyLight® 550 conjugated antibody. However we have seen positive staining in thymus isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is ICOS supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-12-28
Answer
From literature, thymus does express ICOS. Generally ICOS expresses in isoform 1: cell membrane. Regarding which tissues have ICOS expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 11006126, 15489334
Thymus, Pubmed ID: 10779774
Boster Scientific Support
Answered: 2017-12-28
Question
I see that the anti-Human ICOS DyLight® 550 conjugated antibody A00291-Dyl550 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
O. Gonzalez
Verified customer
Asked: 2015-11-06
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-11-06
