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Product Info Summary
| SKU: | A01014-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-BCMA/Tnfrsf17 Antibody Picoband®
View all BCMA/TNFRSF17 Antibodies
SKU/Catalog Number
A01014-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BCMA/Tnfrsf17 Antibody Picoband® catalog # A01014-3. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BCMA/Tnfrsf17 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01014-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat BCMA recombinant protein (Position: M1-T49).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01014-3 is reactive to Tnfrsf17 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
20 kDa
Calculated molecular weight
20.165kDa
Background of BCMA/TNFRSF17
B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01014-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat spleen tissue, rat thymus tissue, rat lung tissue, rat testis tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of BCMA using anti-BCMA antibody (A01014-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: rat testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCMA antigen affinity purified polyclonal antibody (Catalog # A01014-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCMA at approximately 20KD. The expected band size for BCMA is at 20KD.
Protein Target Info & Infographic
Gene/Protein Information For Tnfrsf17 (Source: Uniprot.org, NCBI)
Gene Name
Tnfrsf17
Full Name
Tumor necrosis factor receptor superfamily member 17
Weight
20.165kDa
Alternative Names
TNF receptor superfamily member 17 TNFRSF17 BCM, BCMA, CD269, TNFRSF13A TNF receptor superfamily member 17 tumor necrosis factor receptor superfamily member 17|B cell maturation |B-cell maturation factor|B-cell maturation protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tnfrsf17, check out the Tnfrsf17 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tnfrsf17: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BCMA/Tnfrsf17 Antibody Picoband® (A01014-3)
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Customer Q&As
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1 Customer Q&As for Anti-BCMA/Tnfrsf17 Antibody Picoband®
Question
We are currently using anti-BCMA/Tnfrsf17 antibody A01014-3 for rat tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-16
Answer
The anti-BCMA/Tnfrsf17 antibody (A01014-3) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-16
