Anti-SP6 Antibody Picoband™

Product Name

Anti-SP6 Antibody Picoband™

View all SP6 Antibodies

SKU/Catalog Number

A08730-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SP6 Antibody Picoband™ catalog # A08730-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SP6 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08730-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SP6 recombinant protein (Position: H18-N376).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A08730-3 is reactive to SP6 in Human

Applications

A08730-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

43 kDa

Calculated molecular weight

Background of SP6

SP6 belongs to a family of transcription factors that contain 3 classical zinc finger DNA-binding domains consisting of a zinc atom tetrahedrally coordinated by 2 cysteines and 2 histidines (C2H2 motif). These transcription factors bind to GC-rich sequences and related GT and CACCC boxes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SP6 using anti-SP6 antibody (A08730-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP6 antigen affinity purified polyclonal antibody (Catalog # A08730-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SP6 at approximately 43 kDa. The expected band size for SP6 is at 43 kDa.

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Figure 2. Flow Cytometry analysis of K562 cells using anti-SP6 antibody (A08730-3).
Overlay histogram showing K562 cells stained with A08730-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SP6 Antibody (A08730-3, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For SP6 (Source: Uniprot.org, NCBI)

Alternative Names

Epfn; EPIPROFIN; KLF14MGC119663; Krueppel-like factor 14; Kruppel-like factor 14; MGC119662; MGC119664; MGC119665; Sp6 transcription factor; transcription factor Sp6 SP6 EPFN, EPIPROFIN, KLF14 Sp6 transcription factor transcription factor Sp6|Kruppel-like factor 14|krueppel-like factor 14

For more info on SP6, check out the SP6 Infographic

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In this infographic, you will see the following information for SP6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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