Anti-Human IDE DyLight® 550 conjugated Antibody

Product Name

Anti-Human IDE DyLight® 550 conjugated Antibody

View all Insulysin/IDE Antibodies

SKU/Catalog Number

A01358-Dyl550

Size

100 μg/vial

Form

Liquid

Description

Boster Bio Anti-Human IDE DyLight® 550 conjugated Antibody catalog # A01358-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Cite This Product

Anti-Human IDE DyLight® 550 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01358-Dyl550)

Host

Rabbit

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human IDE recombinant protein (Position: F485-K756).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01358-Dyl550 is reactive to IDE in Human

Reconstitution

Observed Molecular Weight

39 kDa

Calculated molecular weight

117.968kDa

Background of Insulysin/IDE

Insulin-degrading enzyme, also known as IDE, is an enzyme. This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01358-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells

Validation Images & Assay Conditions

Gene/Protein Information For IDE (Source: Uniprot.org, NCBI)

Alternative Names

Insulin-degrading enzyme; Abeta-degrading protease; Insulin protease; Insulinase; Insulysin; IDE IDE INSULYSIN insulin degrading enzyme insulin-degrading enzyme|Abeta-degrading protease|insulin protease|insulinase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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