Product Info Summary
SKU: | PB9073 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-15 Lipoxygenase 1/ALOX15 Antibody Picoband®
View all 15-Lipoxygenase 1 Antibodies
SKU/Catalog Number
PB9073
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-15 Lipoxygenase 1/ALOX15 Antibody Picoband® catalog # PB9073. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-15 Lipoxygenase 1/ALOX15 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9073)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ALOX15 recombinant protein (Position: G2-P337). Human ALOX15 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat ALOX15, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9073 is reactive to ALOX15 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
75 kDa
Calculated molecular weight
74804 MW
Background of 15-Lipoxygenase 1
Arachidonate 15-lipoxygenase, also known as 15 LOX or LOG15, is an enzyme that in humans is encoded by the ALOX15 gene. This gene is mapped to 17p13.2. It is found that ALOX15 is a mutator gene. ALOX15 gene product is implicated in antiinflammation, membrane remodeling, and cancer development/metastasis. Loss of the TP53 gene, or gain-of-function activities resulting from the expression of its mutant forms regulates ALOX15 promoter activity in human and in mouse, albeit in directionally opposite manners.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9073 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Positive Control
WB: rat lung tissue, rat thymus tissue, rat spleen tissue, mouse lung tissue, mouse testis tissue, mouse thymus tissue
IHC: mouse lung tissue, rat lung tissue, human intestine cancer tissue, human mammary cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC analysis of ALOX15 using anti-ALOX15 antibody (PB9073).
ALOX15 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ALOX15 Antibody (PB9073) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 2. IHC analysis of ALOX15 using anti-ALOX15 antibody (PB9073).
ALOX15 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ALOX15 Antibody (PB9073) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of ALOX15 using anti-ALOX15 antibody (PB9073).
ALOX15 was detected in a paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ALOX15 Antibody (PB9073) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of ALOX15 using anti-ALOX15 antibody (PB9073).
ALOX15 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ALOX15 Antibody (PB9073) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. Western blot analysis of ALOX15 using anti-ALOX15 antibody (PB9073).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: rat spleen tissue lysates,
Lane 4: mouse lung tissue lysates,
Lane 5: mouse testis tissue lysates,
Lane 6: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALOX15 antigen affinity purified polyclonal antibody (Catalog # PB9073) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALOX15 at approximately 75 kDa. The expected band size for ALOX15 is at 75 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ALOX15 (Source: Uniprot.org, NCBI)
Gene Name
ALOX15
Full Name
Arachidonate 15-lipoxygenase
Weight
74804 MW
Superfamily
lipoxygenase family
Alternative Names
Arachidonate 15-lipoxygenase;15-LOX;15-LOX-1;1.13.11.33;12/15-lipoxygenase;Arachidonate 12-lipoxygenase, leukocyte-type;12-LOX;1.13.11.31;Arachidonate omega-6 lipoxygenase;ALOX15;LOG15; ALOX15 12-LOX, 15-LOX, 15-LOX-1, LOG15 arachidonate 15-lipoxygenase polyunsaturated fatty acid lipoxygenase ALOX15|12/15-lipoxygenase|15-LOX type 1|15-lipooxygenase-1|15-lipoxygenase type 1|arachidonate 12-lipoxygenase, leukocyte-type|arachidonate omega-6 lipoxygenase|hepoxilin A3 synthase Alox15|linoleate 13S-lipoxygenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ALOX15, check out the ALOX15 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ALOX15: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-15 Lipoxygenase 1/ALOX15 Antibody Picoband® (PB9073)
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Customer Q&As
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6 Customer Q&As for Anti-15 Lipoxygenase 1/ALOX15 Antibody Picoband®
Question
We are currently using anti-15 Lipoxygenase 1/ALOX15 antibody PB9073 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
J. Parker
Verified customer
Asked: 2020-03-04
Answer
The anti-15 Lipoxygenase 1/ALOX15 antibody (PB9073) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-04
Question
See below the WB image, lot number and protocol we used for tongue using anti-15 Lipoxygenase 1/ALOX15 antibody PB9073. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-07
Question
Would anti-15 Lipoxygenase 1/ALOX15 antibody PB9073 work for WB with tongue?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
According to the expression profile of tongue, ALOX15 is highly expressed in tongue. So, it is likely that anti-15 Lipoxygenase 1/ALOX15 antibody PB9073 will work for WB with tongue.
Boster Scientific Support
Answered: 2019-08-07
Question
Would anti-15 Lipoxygenase 1/ALOX15 antibody PB9073 work on monkey IHC-P with brain lung?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
Our lab technicians have not tested anti-15 Lipoxygenase 1/ALOX15 antibody PB9073 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-15 Lipoxygenase 1/ALOX15 antibody PB9073 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey brain lung in IHC-P, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-24
Question
Is this PB9073 anti-15 Lipoxygenase 1/ALOX15 antibody reactive to the isotypes of ALOX15?
Verified Customer
Verified customer
Asked: 2019-02-08
Answer
The immunogen of PB9073 anti-15 Lipoxygenase 1/ALOX15 antibody is E.coli-derived human ALOX15 recombinant protein (Position: G2-P337). Human ALOX15 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat ALOX15, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-08
Question
Is a blocking peptide available for product anti-15 Lipoxygenase 1/ALOX15 antibody (PB9073)?
Verified Customer
Verified customer
Asked: 2018-04-19
Answer
We do provide the blocking peptide for product anti-15 Lipoxygenase 1/ALOX15 antibody (PB9073). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-04-19