Click image to see more details
Product Info Summary
| SKU: | M01358 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-IDE/Insulysin Rabbit Monoclonal Antibody
View all Insulysin/IDE Antibodies
SKU/Catalog Number
M01358
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IDE/Insulysin Rabbit Monoclonal Antibody catalog # M01358. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IDE/Insulysin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01358)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOOB-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IDE
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01358 is reactive to IDE in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
118 kDa
Calculated molecular weight
117968 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01358 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:100
Positive Control
WB: human HepG2 whole cell, human U251 whole cell, human U2OS whole cell, human SH-SY5Y whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of IDE using anti-IDE antibody (M01358).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human U2OS whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDE antigen affinity purified monoclonal antibody (M01358) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDE at approximately 118 kDa. The expected band size for IDE is at 118 kDa.
Protein Target Info & Infographic
Gene/Protein Information For IDE (Source: Uniprot.org, NCBI)
Gene Name
IDE
Full Name
Insulin-degrading enzyme
Weight
117968 MW
Superfamily
peptidase M16 family
Alternative Names
Insulin-degrading enzyme;3.4.24.56;Abeta-degrading protease;Insulin protease;Insulinase;Insulysin;IDE; IDE INSULYSIN insulin degrading enzyme insulin-degrading enzyme|Abeta-degrading protease|insulin protease|insulinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IDE, check out the IDE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IDE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IDE/Insulysin Rabbit Monoclonal Antibody (M01358)
Hello CJ!
No publications found for M01358
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-IDE/Insulysin Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-IDE/Insulysin Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
