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Product Info Summary
| SKU: | P00090-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Phospho-Smad2 (S250) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00090-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Smad2 (S250) Rabbit Monoclonal Antibody catalog # P00090-1. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Smad2 (S250) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00090-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HBC-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Smad2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00090-1 is reactive to SMAD2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
100 kDa
Calculated molecular weight
52306 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00090-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
FC 1:20
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human HT-1080 whole cell, rat heart tissue, rat skeletal muscle tissue, mouse heart tissue, mouse skeletal muscle tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Phospho-Smad2 (S250) using anti-Phospho-Smad2 (S250) antibody (P00090-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human HT-1080 whole cell lysates,
Lane 4: rat heart tissue lysates,
Lane 5: rat skeletal muscle tissue lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospho-Smad2 (S250) antigen affinity purified monoclonal antibody (Catalog # P00090-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Phospho-Smad2 (S250) at approximately 58 kDa. The expected band size for Phospho-Smad2 (S250) is at 52 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SMAD2 (Source: Uniprot.org, NCBI)
Gene Name
SMAD2
Full Name
Mothers against decapentaplegic homolog 2
Weight
52306 MW
Superfamily
dwarfin/SMAD family
Alternative Names
Mothers against decapentaplegic homolog 2;MAD homolog 2;Mothers against DPP homolog 2;JV18-1;Mad-related protein 2;hMAD-2;SMAD family member 2;SMAD 2;Smad2;hSMAD2;SMAD2;MADH2, MADR2; SMAD2 JV18, JV18-1, MADH2, MADR2, hMAD-2, hSMAD2 SMAD family member 2 mothers against decapentaplegic homolog 2|MAD homolog 2|SMAD, mothers against DPP homolog 2|Sma- and Mad-related protein 2|mother against DPP homolog 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMAD2, check out the SMAD2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMAD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Smad2 (S250) Rabbit Monoclonal Antibody (P00090-1)
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Customer Q&As
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5 Customer Q&As for Anti-Phospho-Smad2 (S250) Rabbit Monoclonal Antibody
Question
We ordered your anti-Phospho-Smad2 (S250) Rabbit Monoclonal antibody for WB on pancreas spleen a few years ago. I am using mouse, and We want to use the antibody for Flow Cytometry next. We want examining pancreas spleen as well as cervix carcinoma erythroleukemia in our next experiment. Could give a recommendation on which antibody would work the best for Flow Cytometry?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
I have checked the website and datasheets of our anti-Phospho-Smad2 (S250) Rabbit Monoclonal antibody and it appears that P00090-1 has been validated on mouse in both WB and Flow Cytometry. Thus P00090-1 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-30
Question
My colleagues were satisfied with the WB result of your anti-Phospho-Smad2 (S250) Rabbit Monoclonal antibody. However we have seen positive staining in colon adenocarcinoma cytoplasm using this antibody. Is that expected? Could you tell me where is SMAD2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
From literature, colon adenocarcinoma does express SMAD2. Generally SMAD2 expresses in cytoplasm. Regarding which tissues have SMAD2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Chronic myeloid leukemia cell, Pubmed ID: 8980228
Colon adenocarcinoma, Pubmed ID: 9702198
Kidney, Pubmed ID: 8752209
Kidney, Pancreas, and Spleen, Pubmed ID: 15489334
Placenta, Pubmed ID: 8774881
Boster Scientific Support
Answered: 2020-02-21
Question
We are currently using anti-Phospho-Smad2 (S250) Rabbit Monoclonal antibody P00090-1 for mouse tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
The anti-Phospho-Smad2 (S250) Rabbit Monoclonal antibody (P00090-1) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-03
Question
My question regards using your anti-Phospho-Smad2 (S250) Rabbit Monoclonal antibody for smad protein signal transduction studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
I appreciate your inquiry. This P00090-1 anti-Phospho-Smad2 (S250) Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for Flow Cytometry, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-09
Question
We have been able to see staining in mouse colon adenocarcinoma. Any tips? Is anti-Phospho-Smad2 (S250) Rabbit Monoclonal antibody supposed to stain colon adenocarcinoma positively?
Verified Customer
Verified customer
Asked: 2018-06-19
Answer
From what I have seen in literature colon adenocarcinoma does express SMAD2. From what I have seen in Uniprot.org, SMAD2 is expressed in kidney, placenta, kidney, pancreas spleen, chronic myeloid leukemia cell, colon adenocarcinoma, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have SMAD2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Chronic myeloid leukemia cell, Pubmed ID: 8980228
Colon adenocarcinoma, Pubmed ID: 9702198
Kidney, Pubmed ID: 8752209
Kidney, Pancreas, and Spleen, Pubmed ID: 15489334
Placenta, Pubmed ID: 8774881
Boster Scientific Support
Answered: 2018-06-19
