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Product Info Summary
| SKU: | A00090 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Monkey |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SMAD2 Antibody
SKU/Catalog Number
A00090
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-SMAD2 Antibody (Catalog # A00090). Tested in ELISA, WB applications. This antibody reacts with Human, Monkey.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-SMAD2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00090)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad2 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00090 is reactive to SMAD2 in Human, Monkey
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
46281 MW
Background of Smad2
Smad2 (also known as Mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, Mad2, hMAD-2 or hSMAD2) is a member of the Smad family of proteins which are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively, spliced transcript variants have been observed for this gene. SMAD2 may act as a tumor suppressor in colorectal carcinoma. It positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. SMAD2 may be associated with diseases such as Keloids and Ureteral Disease. Anti-SMAD2 Antibody is useful for researchers interested in SMAD pathways, transcription factor activity, sequence-specific DNA binding, and cancer research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00090 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:100,000
IP: User optimized
WB: 1:1,000 - 1:3,000
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SMAD2 using anti-SMAD2 antibody (A00090).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 antigen affinity purified polyclonal antibody (Catalog # A00090) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for SMAD2.
Protein Target Info & Infographic
Gene/Protein Information For SMAD2 (Source: Uniprot.org, NCBI)
Gene Name
SMAD2
Full Name
Mothers against decapentaplegic homolog 2
Weight
46281 MW
Superfamily
dwarfin/SMAD family
Alternative Names
SMAD2, SMAD-2, SMAD 2, mothers against decapentaplegic homolog 2, MAD homolog 2, Mothers against DPP homolog 2, SMAD family member 2, MADH2, MADH 2, JV18-1 SMAD2 JV18, JV18-1, MADH2, MADR2, hMAD-2, hSMAD2 SMAD family member 2 mothers against decapentaplegic homolog 2|MAD homolog 2|SMAD, mothers against DPP homolog 2|Sma- and Mad-related protein 2|mother against DPP homolog 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMAD2, check out the SMAD2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMAD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SMAD2 Antibody (A00090)
Hello CJ!
A00090 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Mouse hepatic neoplasm formation induced by trace level and low frequency exposure to diethylnitrosamine through %u03B2-catenin signaling pathway
Astragaloside effect on TGF-%u03B21, SMAD2/3, and %u03B1-SMA expression in the kidney tissues of diabetic KKAy mice
Effect of Kuijie Granule on the Expression of TGF-?/Smads Signaling Pathway in Patients with Ulcerative Colitis
Recommended Resources
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- Boster's Pathways Library
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-SMAD2 Antibody
Question
We have observed staining in rat cervix carcinoma erythroleukemia. What should we do? Is anti-SMAD2 antibody supposed to stain cervix carcinoma erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
From what I have seen in literature cervix carcinoma erythroleukemia does express SMAD2. From what I have seen in Uniprot.org, SMAD2 is expressed in kidney, placenta, kidney, pancreas spleen, chronic myeloid leukemia cell, colon adenocarcinoma, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have SMAD2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Chronic myeloid leukemia cell, Pubmed ID: 8980228
Colon adenocarcinoma, Pubmed ID: 9702198
Kidney, Pubmed ID: 8752209
Kidney, Pancreas, and Spleen, Pubmed ID: 15489334
Placenta, Pubmed ID: 8774881
Boster Scientific Support
Answered: 2020-01-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas spleen using anti-SMAD2 antibody A00090. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-19
Question
My boss were content with the WB result of your anti-SMAD2 antibody. However we have observed positive staining in kidney cytoplasm using this antibody. Is that expected? Could you tell me where is SMAD2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
Based on literature, kidney does express SMAD2. Generally SMAD2 expresses in cytoplasm. Regarding which tissues have SMAD2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Chronic myeloid leukemia cell, Pubmed ID: 8980228
Colon adenocarcinoma, Pubmed ID: 9702198
Kidney, Pubmed ID: 8752209
Kidney, Pancreas, and Spleen, Pubmed ID: 15489334
Placenta, Pubmed ID: 8774881
Boster Scientific Support
Answered: 2019-11-05
Question
We ordered your anti-SMAD2 antibody for IP on pancreas spleen a few months ago. I am using rat, and We intend to use the antibody for WB next. I am interested in examining pancreas spleen as well as colon adenocarcinoma in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
I viewed the website and datasheets of our anti-SMAD2 antibody and it appears that A00090 has been validated on rat in both IP and WB. Thus A00090 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-03
Question
Would A00090 anti-SMAD2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
It shows on the product datasheet, A00090 anti-SMAD2 antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-22
Question
I was wanting to use your anti-SMAD2 antibody for IP for human pancreas spleen on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human pancreas spleen identification?
Verified Customer
Verified customer
Asked: 2019-02-12
Answer
You can see on the product datasheet, A00090 anti-SMAD2 antibody has been tested for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human pancreas spleen in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-12
Question
Is there a BSA free version of anti-SMAD2 antibody A00090 available?
M. Mitchell
Verified customer
Asked: 2019-02-12
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-SMAD2 antibody A00090 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-02-12
Question
Can you help my question with product A00090, anti-SMAD2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-02-08
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00090 anti-SMAD2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-02-08
Question
Will anti-SMAD2 antibody A00090 work for IP with pancreas spleen?
A. Baker
Verified customer
Asked: 2019-01-02
Answer
According to the expression profile of pancreas spleen, SMAD2 is highly expressed in pancreas spleen. So, it is likely that anti-SMAD2 antibody A00090 will work for IP with pancreas spleen.
Boster Scientific Support
Answered: 2019-01-02
Question
We are currently using anti-SMAD2 antibody A00090 for human tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-03
Answer
The anti-SMAD2 antibody (A00090) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-03
Question
My question regards to test anti-SMAD2 antibody A00090 on human pancreas spleen for research purposes, then I may be interested in using anti-SMAD2 antibody A00090 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-06-04
Answer
The products we sell, including anti-SMAD2 antibody A00090, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-06-04
Question
Is this A00090 anti-SMAD2 antibody reactive to the isotypes of SMAD2?
E. Zhang
Verified customer
Asked: 2018-03-19
Answer
The immunogen of A00090 anti-SMAD2 antibody is This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad2 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-03-19
Question
Is a blocking peptide available for product anti-SMAD2 antibody (A00090)?
R. Jackson
Verified customer
Asked: 2014-05-06
Answer
We do provide the blocking peptide for product anti-SMAD2 antibody (A00090). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-05-06
Question
I see that the anti-SMAD2 antibody A00090 works with IP, what is the protocol used to produce the result images on the product page?
B. Krishna
Verified customer
Asked: 2014-04-04
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-04-04
Question
Please see the WB image, lot number and protocol we used for pancreas spleen using anti-SMAD2 antibody A00090. Please let me know if you require anything else.
D. Li
Verified customer
Asked: 2014-03-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-03-06
