Click image to see more details
Product Info Summary
| SKU: | M01985-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PROX1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01985-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PROX1 Rabbit Monoclonal Antibody catalog # M01985-1. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PROX1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01985-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOGA-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PROX1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01985-1 is reactive to PROX1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
45 kDa
Calculated molecular weight
83203 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01985-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Positive Control
WB: HepG2 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PROX1 expression in HepG2 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For PROX1 (Source: Uniprot.org, NCBI)
Gene Name
PROX1
Full Name
Prospero homeobox protein 1
Weight
83203 MW
Superfamily
Prospero homeodomain family
Alternative Names
Prospero homeobox protein 1;Homeobox prospero-like protein PROX1;PROX-1;PROX1; PROX1 prospero homeobox 1 prospero homeobox protein 1|homeobox prospero-like protein PROX1|prospero-related homeobox 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PROX1, check out the PROX1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PROX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PROX1 Rabbit Monoclonal Antibody (M01985-1)
Hello CJ!
M01985-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Meng FW,Liu FS,Liu WH,Li L,Jie LL.Formation of new lymphatic vessels in glioma: An immunohistochemical analysis.Neuropathology.2020 Jun;40(3):215-223.doi:10.1111/neup.12625.Epub 2020 Jan 20.PMID:31960509;PMCID:PMC 7317190.
Species: Human
M01985-1 usage in article: APP:IHC, SAMPLE:GILOMA TISSUE, DILUTION:1:100
Fucoidan inhibits lymphangiogenesis by downregulating the expression of VEGFR3 and PROX1 in human lymphatic endothelial cells
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-PROX1 Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-PROX1 Rabbit Monoclonal antibody for WB for human embryonic brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human embryonic brain identification?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
As indicated on the product datasheet, M01985-1 anti-PROX1 Rabbit Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human embryonic brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-12
Question
See attached the WB image, lot number and protocol we used for embryonic brain using anti-PROX1 Rabbit Monoclonal antibody M01985-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-27
Question
Is this M01985-1 anti-PROX1 Rabbit Monoclonal antibody reactive to the isotypes of PROX1?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
The immunogen of M01985-1 anti-PROX1 Rabbit Monoclonal antibody is A synthesized peptide derived from human PROX1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-02
Question
Is a blocking peptide available for product anti-PROX1 Rabbit Monoclonal antibody (M01985-1)?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
We do provide the blocking peptide for product anti-PROX1 Rabbit Monoclonal antibody (M01985-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-15
Question
My boss were content with the WB result of your anti-PROX1 Rabbit Monoclonal antibody. However we have observed positive staining in embryonic brain nucleus. using this antibody. Is that expected? Could you tell me where is PROX1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
From literature, embryonic brain does express PROX1. Generally PROX1 expresses in nucleus. Regarding which tissues have PROX1 expression, here are a few articles citing expression in various tissues:
Embryonic brain, Pubmed ID: 8812486
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-09-27
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic brain using anti-PROX1 Rabbit Monoclonal antibody M01985-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-07
Question
I am interested in using your anti-PROX1 Rabbit Monoclonal antibody for response to nutrient levels studies. Has this antibody been tested with western blotting on hepg2 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
We appreciate your inquiry. This M01985-1 anti-PROX1 Rabbit Monoclonal antibody is tested on hepg2 cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-29
Question
I see that the anti-PROX1 Rabbit Monoclonal antibody M01985-1 works with WB, what is the protocol used to produce the result images on the product page?
V. Anderson
Verified customer
Asked: 2019-03-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-03-11
Question
We are currently using anti-PROX1 Rabbit Monoclonal antibody M01985-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
T. Zhao
Verified customer
Asked: 2019-01-08
Answer
The anti-PROX1 Rabbit Monoclonal antibody (M01985-1) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-08
Question
We have observed staining in human liver. Do you have any suggestions? Is anti-PROX1 Rabbit Monoclonal antibody supposed to stain liver positively?
W. Banerjee
Verified customer
Asked: 2018-11-06
Answer
According to literature liver does express PROX1. According to Uniprot.org, PROX1 is expressed in liver, embryonic brain, muscle, among other tissues. Regarding which tissues have PROX1 expression, here are a few articles citing expression in various tissues:
Embryonic brain, Pubmed ID: 8812486
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-11-06
Question
Is there a BSA free version of anti-PROX1 Rabbit Monoclonal antibody M01985-1 available?
Verified Customer
Verified customer
Asked: 2018-02-01
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PROX1 Rabbit Monoclonal antibody M01985-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-02-01
Question
Will anti-PROX1 Rabbit Monoclonal antibody M01985-1 work for WB with embryonic brain?
Verified Customer
Verified customer
Asked: 2017-06-15
Answer
According to the expression profile of embryonic brain, PROX1 is highly expressed in embryonic brain. So, it is likely that anti-PROX1 Rabbit Monoclonal antibody M01985-1 will work for WB with embryonic brain.
Boster Scientific Support
Answered: 2017-06-15
Question
My lab would like to test anti-PROX1 Rabbit Monoclonal antibody M01985-1 on human embryonic brain for research purposes, then I may be interested in using anti-PROX1 Rabbit Monoclonal antibody M01985-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Krishna
Verified customer
Asked: 2017-04-12
Answer
The products we sell, including anti-PROX1 Rabbit Monoclonal antibody M01985-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-04-12
Question
Can you help my question with product M01985-1, anti-PROX1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Banerjee
Verified customer
Asked: 2015-04-22
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01985-1 anti-PROX1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-04-22
Question
Does M01985-1 anti-PROX1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Zhang
Verified customer
Asked: 2014-08-27
Answer
You can see on the product datasheet, M01985-1 anti-PROX1 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-08-27
