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Product Info Summary
| SKU: | EK1742 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). |
Product info
Product Name
Rat IL6ST ELISA Kit PicoKine®
View all gp130/CD130 ELISA kits
SKU/Catalog Number
EK1742
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat IL6ST ELISA Kit PicoKine® (96 Tests). Quantitate Rat Il6st in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 0.05ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat IL6ST ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1742)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q23-E618
Sensitivity
<0.05 ng/ml
Assay Range
0.78 ng/ml - 50 ng/ml
Standard Dilution Instructions
See datasheet of EK1742 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1742 is reactive to Il6st in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).
Application Guarantee
EK1742 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of gp130/CD130
GP130 (Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein which is the founding member of the class of all cytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130 is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membranebound gp130. Gp130 is an important part of many different types of signaling complexes. Gp130 molecules with mutations in either of these segments could not transduce a growth signal.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1742-CAP | Anti-Rat Il6st Pre-coated 96-well strip microplate | 1 |
| EK1742-ST | Rat Il6st Standard | 2 vials, 50 ng/tube |
| EK1742-DA | Rat Il6st Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.008 | 0.329 | 0.516 | 0.817 | 1.102 | 1.336 | 1.568 | 1.839 |
Data Example Images
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Rat IL6ST PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect gp130/CD130, Dilution ratio of 1:10, concentration in serum and plasma is around 40 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat IL6ST ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 1254 | 4579 | 20188 | 1322 | 5546 | 21456 |
| Standard deviation | 66.46 | 290.3 | 847.9 | 99.15 | 321.7 | 1652.1 |
| CV (%) | 5.3% | 6.1% | 4.2% | 7.5% | 5.8% | 7.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of gp130/CD130 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1254 | 1275 | 1273 | 1502 | 1326 | 101.94 | 7.6% |
| Sample 2 | 4597 | 4887 | 4558 | 4251 | 4573 | 225.32 | 4.9% |
| Sample 3 | 20188 | 20369 | 19931 | 19988 | 20119 | 173.03 | 0.8% |
Protein Target Info & Infographic
Gene/Protein Information For Il6st (Source: Uniprot.Org, NCBI)
Gene Name
Il6st
Full Name
Interleukin-6 receptor subunit beta
Weight
103.537kDa
Superfamily
type I cytokine receptor family
Alternative Names
Interleukin-6 receptor subunit beta; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; Interleukin-6 signal transducer; Membrane glycoprotein 130; gp130; Oncostatin-M receptor subunit alpha; CD130; Il6st IL6ST CD130, CDW130, GP130, HIES4, IL-6RB, sGP130 interleukin 6 cytokine family signal transducer interleukin-6 receptor subunit beta|CD130 |IL-6 receptor subunit beta|IL-6R subunit beta|gp130 of the rheumatoid arthritis ic peptide-bearing soluble form|gp130, oncostatin M receptor|interleukin receptor beta chain|interleukin-6 signal transducer|membrane glycoprotein 130|membrane glycoprotein gp130|oncostatin-M receptor subunit alpha
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Il6st, check out the Il6st Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Il6st: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat IL6ST ELISA Kit PicoKine® (EK1742)
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Customer Q&As
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8 Customer Q&As for Rat IL6ST ELISA Kit PicoKine®
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-08-28
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-08-28
Question
Q: What is the optimal O.D. value for CD130 ELISA kit? I performed your CD130 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CD130 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-08-02
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD130 ELISA kit to have sensitivity of 0.05ng/ml, that means the minimum amount of CD130 that can be declared/interpreted as positive by the above standard is 0.05ng/ml.
Boster Scientific Support
Answered: 2020-08-02
Question
Q: can I use heparin plasma as samples in Rat CD130 Picokine® ELISA Kit (Catalog # EK1742)?
Verified Customer
Verified customer
Asked: 2020-06-19
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of CD130 causing disruption of its protein structure. CD130 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CD130 ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-06-19
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-03-08
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-03-08
Question
Q: how can I thaw whole blood sample for CD130 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-10-30
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD130 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-10-30
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring CD130 using the EK1742 Rat CD130 Picokine® ELISA Kit?
C. Maharaj
Verified customer
Asked: 2018-10-16
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2018-10-16
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
L. Banerjee
Verified customer
Asked: 2016-10-16
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2016-10-16
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2014-04-16
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2014-04-16
