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Key=sku, value=EK0478
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Key=created_at, value=2014-05-20 07:17:43
Key=updated_at, value=2024-09-26 00:43:56
Key=mst_search_weight, value=0
Key=status, value=Enabled
Key=name, value=Human OSM/Oncostatin M ELISA Kit PicoKine®
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Key=meta_title, value=Human OSM/Oncostatin M ELISA Kit Price PicoKine® | BosterBio
Key=meta_description, value=Human OSM/Oncostatin M ELISA Kit PicoKine® (96 Tests). Quantitate Human OSM in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 10pg/ml.
Key=image, value=/e/k/ek0478.png
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Key=url_key, value=human-osm-oncostatin-m-picokine-trade-elisa-kit-ek0478-boster
Key=url_path, value=human-osm-oncostatin-m-picokine-trade-elisa-kit-ek0478-boster
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Key=image_label, value=Human OSM/Oncostatin M PicoKine ELISA Kit standard curve
Key=small_image_label, value=Human OSM/Oncostatin M PicoKine ELISA Kit standard curve
Key=thumbnail_label, value=Human OSM/Oncostatin M PicoKine ELISA Kit standard curve
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Key=size, value=96 wells/kit, with removable strips.
Key=protein_name, value=Oncostatin-M
Key=gene_full_name, value=Oncostatin-M
Key=uniprot_id, value=P13725
Key=reconstitution
Key=assay_range, value=15.6 pg/ml - 1,000 pg/ml
Key=sensitivity, value=<10 pg/ml
Key=applications, value=ELISA
Key=reactivity, value=Human
Key=form
Key=concentration
Key=cross_reactivity, value=There is no detectable cross-reactivity with other relevant proteins.
Key=recommended_detection_systems
Key=clone_number
Key=principle, value=monoclonal antibody from mouse|polyclonal antibody from goat
Key=anticoagulant, value=heparin or EDTA
Key=assay_procedure_step_1, value=1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml, 15.6 pg/ml
Key=tmb_incubation_time, value=15-25min
Key=normal_range_citations, value=24600984
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Key=source_company, value=Boster EK0478
Key=isotype
Key=molecular_weight, value=28484 MW
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Key=related_product_skus, value=PROTP13725,PROTP13725-1,PROTP53347,PROTQ65Z15,PROTP13725-2,PROTP13725-3,PROTP13725-4
Key=product_category, value=Picokine ELISA Kits
Key=research_category, value=Cytokines, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, Interleukins, Nuclear Signaling, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway
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Key=cj_related_products, value=Kaposi Sarcoma:FEK0577|PROTQ00560|PA1668|EK0479|PB9732|EZ0478
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Key=description, value=Human OSM/Oncostatin M ELISA Kit PicoKine® (96 Tests). Quantitate Human OSM in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=short_description, value=Human OSM/Oncostatin M ELISA Kit PicoKine® (96 Tests). Quantitate Human OSM in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=meta_keyword, value=Human OSM/Oncostatin M ELISA Kit PicoKine, Bosterbio High Sensitivity
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Key=contents
Key=background, value=OSM (Oncostatin M) is a protein that in humans is encoded by the OSM gene. OSM is a pleiotropic cytokine that belongs to the interleukin 6 group of cytokines. The OSM gene is mapped on 22q12.2. Of these cytokines it most closely resembles leukemia inhibitory factor (LIF) in both structure and function. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM signals through cell surface receptors that contain the protein gp130. The type I receptor is composed of gp130 and LIFR, the type II receptor is composed of gp130 and OSMR.
Key=gene_name, value=OSM
Key=synonyms, value=Oncostatin-M;OSM;OSM;
Key=sample_type, value=cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Key=kit_components, value=10 ng/tube
Key=componentsneeded, value=

• Microplate reader in standard size.
• Automated plate washer.
• Incubator.
• Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
• Clean tubes and Eppendorf tubes.
• Washing buffer (neutral PBS or TBS).
• Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
• Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Key=intra_inter_assay_cv, value=

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	40	159	453	36	158	453
Standard deviation	1.96	11.6	29.44	2.19	13.27	36.69
CV (%)	4.9%	7.3%	6.5%	6.1%	8.4%	8.1%

Key=application_details, value=Dilution ratio of 1:1, concentration in serum and plasma is 20-300 pg/ml.
Key=subcellular_localization, value= Secreted.
Key=sequence_similarities
Key=tissue_specificity
Key=protein_function, value= Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration (By similarity). .
Key=application_notes
Key=sample_data, value=

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.008	0.070	0.125	0.266	0.501	0.969	1.516	2.029

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Key=competitor_equivalent_skus, value=sc 27104|sc 50294|sc 5488|sc 374040|sc 373734|sc 133229|sc 374039|sc 129|sc 50296|sc 365136|sc 390253
Key=immunogen, value=Expression system for standard: E.coli; Immunogen sequence: A26R-220
Key=purification
Key=storage, value=Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Key=reproducibility, value=

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	40	39	42	36	39	2.16	5.5%
Sample 2	159	178	156	178	167	10.3	6.1%
Sample 3	453	484	457	488	470	15.62	3.3%

*number of samples for each test n=16.
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