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Product Info Summary
| SKU: | PA2229 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Prospero homeobox protein 1 PROX1 Antibody
SKU/Catalog Number
PA2229
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Prospero homeobox protein 1 PROX1 Antibody catalog # PA2229. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Prospero homeobox protein 1 PROX1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2229)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Standard Protein
A synthetic peptide corresponding to a sequence in the middle region of human PROX1, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2229 is reactive to PROX1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
83203 MW
Background of PROX1
Prospero homeobox protein 1, also called PROX1 is a protein that in humans is encoded by the PROX1 gene. This gene is mapped to 1q32.3. The protein encoded by this gene is a member of the homeobox transcription factor family. Members of this family contain a homeobox domain that consists of a 60-amino acid helix-turn-helix structure that binds DNA and RNA. The protein encoded by this gene is conserved across vertebrates and may play an essential role during development. Altered levels of this protein have been reported in cancers of different organs, such as colon, brain, blood, breast, pancreas, liver and esophagus.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2229 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Thymus Tissue, HELA Cell, JURKAT Cell, MM231 Cell,
Validation Images & Assay Conditions
Click image to see more details
Anti-PROX1 antibody, PA2229, Western blotting
Lane 1: Rat Thymus Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: JURKAT Cell Lysate
Lane 4: MM231 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For PROX1 (Source: Uniprot.org, NCBI)
Gene Name
PROX1
Full Name
Prospero homeobox protein 1
Weight
83203 MW
Superfamily
Prospero homeodomain family
Alternative Names
Homeobox prospero-like protein PROX1; prospero homeobox 1; prospero homeobox protein 1; prospero-related homeobox 1; Prox1; PROX-1 PROX1 prospero homeobox 1 prospero homeobox protein 1|homeobox prospero-like protein PROX1|prospero-related homeobox 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PROX1, check out the PROX1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PROX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Prospero homeobox protein 1 PROX1 Antibody (PA2229)
Hello CJ!
PA2229 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Meng FW,Jing XN,Song GH,Jie LL,Shen FF.Prox1 induces new lymphatic vessel formation and promotes nerve reconstruction in a mouse model of sciatic nerve crush injury.J Anat.2020 Nov;237(5):933-940.doi: 10.1111/joa.13247.Epub 2020 Jun 9.PMID:32515838;PMCID:
Species: Mouse
PA2229 usage in article: APP:IF, SAMPLE:SCIATIC NERVE TISSUE, DILUTION:1:100
Fucoidan inhibits lymphangiogenesis by downregulating the expression of VEGFR3 and PROX1 in human lymphatic endothelial cells
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Prospero homeobox protein 1 PROX1 Antibody
Question
you antibody using your anti-PROX1 antibody for ventricular cardiac muscle tissue morphogenesis studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
Thank you for your inquiry. This PA2229 anti-PROX1 antibody is tested on rat thymus tissue, tissue lysate, hela cell lysate, jurkat cell lysate, mm231 cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-23
Question
We have observed staining in human liver. Are there any suggestions? Is anti-PROX1 antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2019-05-06
Answer
From what I have seen in literature liver does express PROX1. From what I have seen in Uniprot.org, PROX1 is expressed in liver, embryonic brain, muscle, among other tissues. Regarding which tissues have PROX1 expression, here are a few articles citing expression in various tissues:
Embryonic brain, Pubmed ID: 8812486
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-05-06
Question
Our team were happy with the WB result of your anti-PROX1 antibody. However we have observed positive staining in muscle nucleus. using this antibody. Is that expected? Could you tell me where is PROX1 supposed to be expressed?
Z. Jha
Verified customer
Asked: 2018-12-18
Answer
According to literature, muscle does express PROX1. Generally PROX1 expresses in nucleus. Regarding which tissues have PROX1 expression, here are a few articles citing expression in various tissues:
Embryonic brain, Pubmed ID: 8812486
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-12-18
Question
We are currently using anti-PROX1 antibody PA2229 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
S. Carter
Verified customer
Asked: 2014-06-20
Answer
The anti-PROX1 antibody (PA2229) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-06-20
