Anti-Prospero homeobox protein 1 PROX1 Antibody Picoband®

PROX1 antibody

Boster Bio Anti-Prospero homeobox protein 1 PROX1 Antibody catalog # PA2229. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 2 publication(s).

Product Info Summary

SKU: PA2229
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Prospero homeobox protein 1 PROX1 Antibody Picoband®

View all PROX1 Antibodies

SKU/Catalog Number

PA2229

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Prospero homeobox protein 1 PROX1 Antibody catalog # PA2229. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Prospero homeobox protein 1 PROX1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2229)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human PROX1, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2229 is reactive to PROX1 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

55 kDa

Calculated molecular weight

83203 MW

Background of PROX1

Prospero homeobox protein 1, also called PROX1 is a protein that in humans is encoded by the PROX1 gene. This gene is mapped to 1q32.3. The protein encoded by this gene is a member of the homeobox transcription factor family. Members of this family contain a homeobox domain that consists of a 60-amino acid helix-turn-helix structure that binds DNA and RNA. The protein encoded by this gene is conserved across vertebrates and may play an essential role during development. Altered levels of this protein have been reported in cancers of different organs, such as colon, brain, blood, breast, pancreas, liver and esophagus.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2229 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

WB: Rat Thymus Tissue, HELA Cell, JURKAT Cell, MM231 Cell,

Validation Images & Assay Conditions

Gene/Protein Information For PROX1 (Source: Uniprot.org, NCBI)

Gene Name

PROX1

Full Name

Prospero homeobox protein 1

Weight

83203 MW

Superfamily

Prospero homeodomain family

Alternative Names

Prospero homeobox protein 1;Homeobox prospero-like protein PROX1;PROX-1;PROX1; PROX1 prospero homeobox 1 prospero homeobox protein 1|homeobox prospero-like protein PROX1|prospero-related homeobox 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PROX1, check out the PROX1 Infographic

PROX1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PROX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA2229 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Meng FW,Jing XN,Song GH,Jie LL,Shen FF.Prox1 induces new lymphatic vessel formation and promotes nerve reconstruction in a mouse model of sciatic nerve crush injury.J Anat.2020 Nov;237(5):933-940.doi: 10.1111/joa.13247.Epub 2020 Jun 9.PMID:32515838;PMCID:
Species: Mouse
PA2229 usage in article: APP:IF, SAMPLE:SCIATIC NERVE TISSUE, DILUTION:1:100

Fucoidan inhibits lymphangiogenesis by downregulating the expression of VEGFR3 and PROX1 in human lymphatic endothelial cells

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4 Customer Q&As for Anti-Prospero homeobox protein 1 PROX1 Antibody Picoband®

Question

you antibody using your anti-PROX1 antibody for ventricular cardiac muscle tissue morphogenesis studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-04-23

Answer

Thank you for your inquiry. This PA2229 anti-PROX1 antibody is tested on rat thymus tissue, tissue lysate, hela cell lysate, jurkat cell lysate, mm231 cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-04-23

Question

We have observed staining in human liver. Are there any suggestions? Is anti-PROX1 antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2019-05-06

Answer

From what I have seen in literature liver does express PROX1. From what I have seen in Uniprot.org, PROX1 is expressed in liver, embryonic brain, muscle, among other tissues. Regarding which tissues have PROX1 expression, here are a few articles citing expression in various tissues:
Embryonic brain, Pubmed ID: 8812486
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-05-06

Question

Our team were happy with the WB result of your anti-PROX1 antibody. However we have observed positive staining in muscle nucleus. using this antibody. Is that expected? Could you tell me where is PROX1 supposed to be expressed?

Z. Jha

Verified customer

Asked: 2018-12-18

Answer

According to literature, muscle does express PROX1. Generally PROX1 expresses in nucleus. Regarding which tissues have PROX1 expression, here are a few articles citing expression in various tissues:
Embryonic brain, Pubmed ID: 8812486
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-12-18

Question

We are currently using anti-PROX1 antibody PA2229 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?

S. Carter

Verified customer

Asked: 2014-06-20

Answer

The anti-PROX1 antibody (PA2229) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-06-20

Order DetailsPrice
PA2229

100μg

$370
PA2229-10ug

10μg sample (liquid)

$99
PA2229-Biotin

100 μg Biotin conjugated

$570
PA2229-Cy3

100 μg Cy3 conjugated

$570
PA2229-Dylight488

100 μg Dylight488 conjugated

$570
PA2229-Dylight550

100 μg Dylight550 conjugated

$570
PA2229-Dylight594

100 μg Dylight594 conjugated

$570
PA2229-FITC

100 μg FITC conjugated

$570
PA2229-HRP

100 μg HRP conjugated

$570
PA2229-APC

100 μg APC conjugated

$670
PA2229-PE

100 μg PE conjugated

$670
PA2229-iFluor647

100 μg iFluor647 conjugated

$670
PA2229-carrier-free

Carrier Free

$370

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