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Product Info Summary
| SKU: | A01985 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-PROX-1-S514 Antibody
SKU/Catalog Number
A01985
Size
80 µl
Description
Boster Bio Anti-PROX-1-S514 Antibody (Catalog # A01985). Tested in IHC-P, Flow Cytometry, WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PROX-1-S514 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01985)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This PROX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 492-520 amino acids from human PROX1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01985 is reactive to PROX1 in Human, Mouse
Reconstitution
Calculated molecular weight
83203 Da
Background of PROX1
Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01985 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
FC: 1:25
Validation Images & Assay Conditions
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Anti-PROX-1-S514 Antibody at 1:2000 dilution + mouse liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 83 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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PROX-1-S514 Antibody IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PROX-1-S514 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
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Overlay histogram showing A549 cells stained with A01985 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (A01985, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For PROX1 (Source: Uniprot.org, NCBI)
Gene Name
PROX1
Full Name
Prospero homeobox protein 1
Weight
83203 Da
Superfamily
Prospero homeodomain family
Alternative Names
Prospero homeobox protein 1, Homeobox prospero-like protein PROX1, PROX-1, PROX1 PROX1 prospero homeobox 1 prospero homeobox protein 1|homeobox prospero-like protein PROX1|prospero-related homeobox 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PROX1, check out the PROX1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PROX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PROX-1-S514 Antibody (A01985)
Hello CJ!
A01985 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Meng FW,Yu JT,Chen JY,Yang PF.New lymphatic cell formation is associated with damaged brain tissue clearance after penetrating traumatic brain injury.Sci Rep.2021 May 13;11(1):10193.doi:10.1038/s41598-021-89616-3.PMID:33986371;PMCID:PMC8119702.
Species: Mouse
A01985 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:100
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