Product Info Summary
SKU: | A01384-1 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-PKR EIF2AK2 Antibody
SKU/Catalog Number
A01384-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PKR EIF2AK2 Antibody (Catalog # A01384-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PKR antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PKR EIF2AK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01384-1)
Host
Rabbit
Contents
PKR Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-PKR antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human PKR. The immunogen is located within the last 50 amino acids of PKR.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two different isoforms of PKR are known to exist; this antibody will detect both isoforms.
Reactive Species
A01384-1 is reactive to EIF2AK2 in Human, Mouse, Rat
Applications
A01384-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
62094 MW
Background of PKR
The interferon-inducible, double-stranded RNA (dsRNA)-dependent protein kinase PKR is a member of the eukaryotic initiation factor-2 alpha (eIF2-alpha) kinase family, possessing serine-threonine kinase activity and two dsRNA-binding motifs that acts as part of the innate immune system. Upon binding dsRNA, PKR undergoes a conformational change leading to its activation and its phosphorylation of the translation factor eIF2, resulting in a general shutdown of protein synthesis and induction of apoptosis through upregulation of caspase-8 and capsase-9 activity in order to prevent the production of more viruses. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1 μg/mL; IF: 20 μg/mL; IHC-P: 2.5-5 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
![a01384 1 anti pkr eif2ak2 antibody wb testing 1 a01384 1 anti pkr eif2ak2 antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a01384-1-anti-pkr-eif2ak2-antibody-wb-testing-1.jpg)
Click image to see more details
Western Blot Validation of PKR in Human Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: PKR A01384-1 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
![a01384 1 anti pkr eif2ak2 antibody wb testing 2 a01384 1 anti pkr eif2ak2 antibody wb testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a01384-1-anti-pkr-eif2ak2-antibody-wb-testing-2.jpg)
Click image to see more details
Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: PKR A01384-1 (1 μg/mL), PKR, (1 μg/mL, and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
![a01384 1 anti pkr eif2ak2 antibody if testing 3 a01384 1 anti pkr eif2ak2 antibody if testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a01384-1-anti-pkr-eif2ak2-antibody-if-testing-3.jpg)
Click image to see more details
Immunofluorescence Validation of PKR in Mouse Lung
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling PKR with A01384-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
![a01384 1 anti pkr eif2ak2 antibody ihc testing 4 a01384 1 anti pkr eif2ak2 antibody ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a01384-1-anti-pkr-eif2ak2-antibody-ihc-testing-4.jpg)
Click image to see more details
Immunohistochemistry Validation of PKR in Rat Lung
Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-PKR antibody (A01384-1) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
![a01384 1 anti pkr eif2ak2 antibody if testing 5 a01384 1 anti pkr eif2ak2 antibody if testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a01384-1-anti-pkr-eif2ak2-antibody-if-testing-5.jpg)
Click image to see more details
Immunofluorescence Validation of PKR in Mouse Lung
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling PKR with A01384-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
![a01384 1 anti pkr eif2ak2 antibody ihc testing 6 a01384 1 anti pkr eif2ak2 antibody ihc testing 6](https://www.bosterbio.com/media/catalog/product/a/0/a01384-1-anti-pkr-eif2ak2-antibody-ihc-testing-6.jpg)
Click image to see more details
Immunohistochemistry Validation of PKR in Mouse Lung
Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-PKR antibody (A01384-1) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Protein Target Info & Infographic
Gene/Protein Information For EIF2AK2 (Source: Uniprot.org, NCBI)
Gene Name
EIF2AK2
Full Name
Interferon-induced, double-stranded RNA-activated protein kinase
Weight
62094 MW
Superfamily
protein kinase superfamily
Alternative Names
double stranded RNA activated protein kinase; EC 2.7.11.1; eIF-2A protein kinase 2; EIF2AK1; EIF2AK2; eukaryotic translation initiation factor 2-alpha kinase 2P1/eIF-2A protein kinase; interferon-induced, double-stranded RNA-activated protein kinase; interferon-inducible double stranded RNA dependent; interferon-inducible elF2alpha kinase; Interferon-inducible RNA-dependent protein kinase; P68 Kinase; PKR; PKRp68 kinase; PPP1R83; PRKR; PRKRMGC126524; Protein Kinase R; Protein kinase RNA-activated EIF2AK2 EIF2AK1, LEUDEN, PKR, PPP1R83, PRKR eukaryotic translation initiation factor 2 alpha kinase 2 interferon-induced, double-stranded RNA-activated protein kinase|P1/eIF-2A protein kinase|double stranded RNA activated protein kinase|eIF-2A protein kinase 2|interferon-inducible elF2alpha kinase|p68 kinase|protein kinase R|protein kinase, interferon-inducible double stranded RNA dependent|protein phosphatase 1, regulatory subunit 83|tyrosine-protein kinase EIF2AK2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF2AK2, check out the EIF2AK2 Infographic
![EIF2AK2 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF2AK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PKR EIF2AK2 Antibody (A01384-1)
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