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Product Info Summary
| SKU: | A01281-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-TAPA1/Cd81 Antibody Picoband®
SKU/Catalog Number
A01281-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TAPA1/Cd81 Antibody Picoband® catalog # A01281-3. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TAPA1/Cd81 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01281-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse TAPA1 recombinant protein (Position: K116-K201).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01281-3 is reactive to Cd81 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22 kDa
Calculated molecular weight
25.809kDa
Background of CD81
CD81 molecule, also known as CD81 (Cluster of Differentiation 81), is a protein which in humans is encoded by the CD81 gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01281-3 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
Immunofluorescence, 5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat brain tissue, mouse ovary tissue, mouse brain tissue, mouse NIH/3T3 whole cell
IHC: rat liver tissue
IF: mouse liver tissue, rat liver tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TAPA1/CD81 using anti-TAPA1/CD81 antibody (A01281-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse ovary tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAPA1/CD81 antigen affinity purified polyclonal antibody (Catalog # A01281-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAPA1/CD81 at approximately 22 kDa. The expected band size for TAPA1/CD81 is at 26 kDa.
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Figure 2. IHC analysis of TAPA1/CD81 using anti-TAPA1/CD81 antibody (A01281-3).
TAPA1/CD81 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TAPA1/CD81 Antibody (A01281-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IF analysis of TAPA1/CD81 using anti-TAPA1/CD81 antibody (A01281-3).
TAPA1/CD81 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TAPA1/CD81 Antibody (A01281-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 4. IF analysis of TAPA1/CD81 using anti-TAPA1/CD81 antibody (A01281-3).
TAPA1/CD81 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TAPA1/CD81 Antibody (A01281-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For Cd81 (Source: Uniprot.org, NCBI)
Gene Name
Cd81
Full Name
CD81 antigen
Weight
25.809kDa
Superfamily
tetraspanin (TM4SF) family
Alternative Names
CD81 antigen; 26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1; CD81; Cd81; Tapa1 CD81 CVID6, S5.7, TAPA1, TSPAN28 CD81 molecule CD81 |26 kDa cell surface protein TAPA-1|CD81 (target of antiproliferative 1)|tetraspanin-28|tspan-28
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cd81, check out the Cd81 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cd81: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TAPA1/Cd81 Antibody Picoband® (A01281-3)
Hello CJ!
A01281-3 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wang D,Hao C,Zhang L,Zhang J,Liu S,Li Y,Qu Y,Zhao Y,Huang R,Wei J,Yao W.Exosomal miR-125a-5p derived from silica-exposed macrophages induces fibroblast transdifferentiation.Ecotoxicol Environ Saf.2020 Apr 1;192:110253.doi:10.1016/j.ecoenv.2020.110253.Epub
Species: Human,Mouse
A01281-3 usage in article: APP:WB, SAMPLE:FIBROBLAST, DILUTION:NA
Cao G,Meng X, Han X,Li J.Exosomes derived from circRNA Rtn4-modified BMSCs attenuate TNF-α-induced cytotoxicity and apoptosis in murine MC3T3-E1 cells by sponging miR-146a.Biosci Rep.2020 May 29;40(5): BSR20193436.doi:10.1042/BSR20193436.PMID:32400849;PMC
Species: Mouse
A01281-3 usage in article: APP:WB, SAMPLE:MC3T3-E1 CELLS, DILUTION:NA
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-TAPA1/Cd81 Antibody Picoband®
Question
I see that the anti-TAPA1/Cd81 antibody A01281-3 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-29
Question
Will anti-TAPA1/Cd81 antibody A01281-3 work for IHC with myometrium?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
According to the expression profile of myometrium, CD81 is highly expressed in myometrium. So, it is likely that anti-TAPA1/Cd81 antibody A01281-3 will work for IHC with myometrium.
Boster Scientific Support
Answered: 2020-01-07
Question
Our lab want to know about to test anti-TAPA1/Cd81 antibody A01281-3 on rat myometrium for research purposes, then I may be interested in using anti-TAPA1/Cd81 antibody A01281-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
The products we sell, including anti-TAPA1/Cd81 antibody A01281-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-27
Question
We have seen staining in mouse myometrium. Are there any suggestions? Is anti-TAPA1/Cd81 antibody supposed to stain myometrium positively?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
According to literature myometrium does express CD81. According to Uniprot.org, CD81 is expressed in myometrium, brain, among other tissues. Regarding which tissues have CD81 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-11-15
Question
We are currently using anti-TAPA1/Cd81 antibody A01281-3 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
The anti-TAPA1/Cd81 antibody (A01281-3) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-08
Question
See attached the WB image, lot number and protocol we used for myometrium using anti-TAPA1/Cd81 antibody A01281-3. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-17
Question
My lab would like using your anti-TAPA1/Cd81 antibody for regulation of cell population proliferation studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-04-24
Answer
We appreciate your inquiry. This A01281-3 anti-TAPA1/Cd81 antibody is tested on mouse brain, brain tissue, tissue lysate. It is guaranteed to work for ELISA, Flow Cytometry, IHC, ICC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-04-24
Question
My boss were content with the WB result of your anti-TAPA1/Cd81 antibody. However we have been able to see positive staining in brain cell membrane using this antibody. Is that expected? Could you tell me where is CD81 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-11-23
Answer
Based on literature, brain does express CD81. Generally CD81 expresses in cell membrane. Regarding which tissues have CD81 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-11-23
Question
Would A01281-3 anti-TAPA1/Cd81 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-10-31
Answer
You can see on the product datasheet, A01281-3 anti-TAPA1/Cd81 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-31
Question
Is this A01281-3 anti-TAPA1/Cd81 antibody reactive to the isotypes of CD81?
Verified Customer
Verified customer
Asked: 2018-10-02
Answer
The immunogen of A01281-3 anti-TAPA1/Cd81 antibody is E. coli-derived mouse TAPA1 recombinant protein (Position: K116-K201). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-10-02
Question
I was wanting to use your anti-TAPA1/Cd81 antibody for IHC for rat myometrium on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat myometrium identification?
V. Moore
Verified customer
Asked: 2018-06-28
Answer
As indicated on the product datasheet, A01281-3 anti-TAPA1/Cd81 antibody has been tested for ELISA, Flow Cytometry, IHC, ICC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat myometrium in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-28
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for myometrium using anti-TAPA1/Cd81 antibody A01281-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-10-20
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-20
Question
Do you have a BSA free version of anti-TAPA1/Cd81 antibody A01281-3 available?
Verified Customer
Verified customer
Asked: 2017-07-19
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-TAPA1/Cd81 antibody A01281-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-19
Question
We have tried in the past anti-TAPA1/Cd81 antibody for Flow Cytometry on brain in the past. I am using mouse, and We are going to use the antibody for ELISA next. I was wanting to use examining brain as well as myometrium in our next experiment. Could give a recommendation on which antibody would work the best for ELISA?
K. Anderson
Verified customer
Asked: 2014-11-20
Answer
I looked at the website and datasheets of our anti-TAPA1/Cd81 antibody and it seems that A01281-3 has been tested on mouse in both Flow Cytometry and ELISA. Thus A01281-3 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-11-20
Question
I have a question about product A01281-3, anti-TAPA1/Cd81 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Huang
Verified customer
Asked: 2013-11-18
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01281-3 anti-TAPA1/Cd81 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-11-18
Question
Is a blocking peptide available for product anti-TAPA1/Cd81 antibody (A01281-3)?
J. Zhao
Verified customer
Asked: 2013-11-04
Answer
We do provide the blocking peptide for product anti-TAPA1/Cd81 antibody (A01281-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-11-04
