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Product Info Summary
| SKU: | EK2003 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse LTBR ELISA Kit PicoKine®
View all Lymphotoxin beta R/TNFRSF3 ELISA kits
SKU/Catalog Number
EK2003
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse LTBR ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ltbr in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse LTBR ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2003)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q31-L223
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2003 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2003 is reactive to Ltbr in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2003 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Lymphotoxin beta R/TNFRSF3
Lymphotoxin beta receptor (LTBR), also known as tumor necrosis factor receptor superfamily member 3 (TNFRSF3), is a cell surface receptor for lymphotoxin involved in apoptosis and cytokine release. This gene is mapped to 6 F3; 6 59.32 cM. This gene encodes a member of the tumor necrosis factor receptor superfamily. The major ligands of this receptor include lymphotoxin alpha/beta and tumor necrosis factor ligand superfamily member 14. The encoded protein plays a role in signalling during the development of lymphoid and other organs, lipid metabolism, immune response, and programmed cell death. Activity of this receptor has also been linked to carcinogenesis. Alternatively spliced transcript variants encoding multiple isoforms have been observed.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2003-CAP | Anti-Mouse Ltbr Pre-coated 96-well strip microplate | 1 |
| EK2003-ST | Mouse Ltbr Standard | 2 vials, 10 ng/tube |
| EK2003-DA | Mouse Ltbr Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.037 | 0.063 | 0.143 | 0.285 | 0.523 | 1.037 | 1.678 | 2.012 |
Data Example Images
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Mouse LTBR PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Lymphotoxin beta R/TNFRSF3, Dilution ratio of 1:1, concentration in serum and plasma is around 60 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse LTBR ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 136 | 552 | 2451 | 141 | 582 | 2471 |
| Standard deviation | 7.07 | 29.26 | 134.81 | 8.18 | 36.08 | 163.09 |
| CV (%) | 5.2% | 5.3% | 5.5% | 5.8% | 6.2% | 6.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Lymphotoxin beta R/TNFRSF3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 136 | 148 | 150 | 127 | 140 | 9.33 | 6.6% |
| Sample 2 | 552 | 557 | 552 | 596 | 564 | 18.44 | 3.2% |
| Sample 3 | 2451 | 2253 | 2433 | 2219 | 2339 | 103.89 | 4.4% |
Protein Target Info & Infographic
Gene/Protein Information For Ltbr (Source: Uniprot.Org, NCBI)
Gene Name
Ltbr
Full Name
Tumor necrosis factor receptor superfamily member 3
Weight
46.709kDa
Alternative Names
Tumor necrosis factor receptor superfamily member 3; Lymphotoxin-beta receptor; Ltbr; Tnfcr; Tnfrsf3 LTBR D12S370, LT-BETA-R, TNF-R-III, TNFCR, TNFR-RP, TNFR2-RP, TNFR3, TNFRSF3 lymphotoxin beta receptor tumor necrosis factor receptor superfamily member 3|lymphotoxin B receptor|lymphotoxin beta receptor (TNFR superfamily, member 3)|tumor necrosis factor C receptor|tumor necrosis factor receptor 2-related protein|tumor necrosis factor receptor type III
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Ltbr, check out the Ltbr Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Ltbr: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse LTBR ELISA Kit PicoKine® (EK2003)
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Customer Q&As
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6 Customer Q&As for Mouse LTBR ELISA Kit PicoKine®
Question
Q: can I use citrate plasma as samples in Mouse Lymphotoxin beta R Picokine® ELISA Kit (Catalog # EK2003)?
Verified Customer
Verified customer
Asked: 2020-07-12
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Lymphotoxin beta R causing degradation of its protein structure. Lymphotoxin beta R may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Lymphotoxin beta R ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-07-12
Question
Q: how to proceed with the analysis of ELISA data? I have obtained Lymphotoxin beta R level in serum.
K. White
Verified customer
Asked: 2019-12-14
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-12-14
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
S. Dayal
Verified customer
Asked: 2019-05-06
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-05-06
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
C. Adams
Verified customer
Asked: 2018-06-13
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-06-13
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-05-16
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-05-16
Question
Q: What is the optimal O.D. value for Lymphotoxin beta R ELISA kit? I used your Lymphotoxin beta R ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Lymphotoxin beta R even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2017-10-03
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Lymphotoxin beta R ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Lymphotoxin beta R that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2017-10-03
