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Product Info Summary
| SKU: | P01384 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Phospho-PKR (T446) EIF2AK2 Rabbit Monoclonal Antibody
SKU/Catalog Number
P01384
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-PKR (T446) EIF2AK2 Rabbit Monoclonal Antibody catalog # P01384. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-PKR (T446) EIF2AK2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01384)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BIC-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-PKR (T446)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P01384 is reactive to EIF2AK2 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
62094 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01384 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
Positive Control
IHC: human colon tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of PKR phosphorylation expression in HeLa cell lysate treated with Calyculin A and TNF-alpha.
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Immunohistochemical analysis of paraffin-embedded human colon, using Phospho-PKR (T446) Antibody.
Protein Target Info & Infographic
Gene/Protein Information For EIF2AK2 (Source: Uniprot.org, NCBI)
Gene Name
EIF2AK2
Full Name
Interferon-induced, double-stranded RNA-activated protein kinase
Weight
62094 MW
Superfamily
protein kinase superfamily
Alternative Names
Interferon-induced, double-stranded RNA-activated protein kinase;2.7.11.1;Eukaryotic translation initiation factor 2-alpha kinase 2;eIF-2A protein kinase 2;Interferon-inducible RNA-dependent protein kinase;P1/eIF-2A protein kinase;Protein kinase RNA-activated;PKR;Protein kinase R ;Tyrosine-protein kinase EIF2AK2;2.7.10.2;p68 kinase;EIF2AK2;PKR, PRKR; EIF2AK2 EIF2AK1, LEUDEN, PKR, PPP1R83, PRKR eukaryotic translation initiation factor 2 alpha kinase 2 interferon-induced, double-stranded RNA-activated protein kinase|P1/eIF-2A protein kinase|double stranded RNA activated protein kinase|eIF-2A protein kinase 2|interferon-inducible elF2alpha kinase|p68 kinase|protein kinase R|protein kinase, interferon-inducible double stranded RNA dependent|protein phosphatase 1, regulatory subunit 83|tyrosine-protein kinase EIF2AK2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF2AK2, check out the EIF2AK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF2AK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-PKR (T446) EIF2AK2 Rabbit Monoclonal Antibody (P01384)
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