Click image to see more details
Product Info Summary
| SKU: | PA2025 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PKR/EIF2AK2 Antibody Picoband®
SKU/Catalog Number
PA2025
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PKR/EIF2AK2 Antibody catalog # PA2025. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKR/EIF2AK2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2025)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human PKR.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2025 is reactive to EIF2AK2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
94 kDa
Calculated molecular weight
62094 MW
Background of PKR
EIF2AK2 (Eukaryotic Translation Initiation Factor 2-Alpha Kinase 2), also called PKR, is an enzyme that in humans is encoded by the EIF2AK2 gene. Activation of EIF2AK2 allows the kinase to phosphorylate its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2, leading to the inhibition of protein synthesis (summary by Kuhen et al., 1996). By FISH analysis, Squire et al. (1993) assigned the EIF2AK2 gene to the boundary between chromosome 2p22-p21. Ben-Asouli et al. (2002) showed that human gamma-interferon mRNA uses local activation of PKR in the cell to control its own translation yield. IFNG mRNA was found to activate PKR through a pseudoknot in its 5-prime untranslated region. Taylor et al. (1999) studied the mechanism underlying the resistance of hepatitis C virus (HCV) to interferon. They demonstrated that the HCV envelope protein E2 contains a sequence identical with phosphorylation sites of the interferon-inducible protein kinase PKR and the translation initiation factor EIF2-alpha, a target of PKR. E2 inhibited the kinase activity of PKR and blocked its inhibitory effect on protein synthesis and cell growth.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2025 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-PKR antibody, PA2025, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human EIF2AK2, 35.0KD
(162aa tag+ M1-K154)
Lane 1: Recombinant Human EIF2AK2 Protein 10ng
Lane 2: Recombinant Human EIF2AK2 Protein 5ng
Lane 3: Recombinant Human EIF2AK2 Protein 2.5ng
Lane 4: Recombinant Human EIF2AK2 Protein 1.25ng
Protein Target Info & Infographic
Gene/Protein Information For EIF2AK2 (Source: Uniprot.org, NCBI)
Gene Name
EIF2AK2
Full Name
Interferon-induced, double-stranded RNA-activated protein kinase
Weight
62094 MW
Superfamily
protein kinase superfamily
Alternative Names
Interferon-induced, double-stranded RNA-activated protein kinase;2.7.11.1;Eukaryotic translation initiation factor 2-alpha kinase 2;eIF-2A protein kinase 2;Interferon-inducible RNA-dependent protein kinase;P1/eIF-2A protein kinase;Protein kinase RNA-activated;PKR;Protein kinase R ;Tyrosine-protein kinase EIF2AK2;2.7.10.2;p68 kinase;EIF2AK2;PKR, PRKR; EIF2AK2 EIF2AK1, LEUDEN, PKR, PPP1R83, PRKR eukaryotic translation initiation factor 2 alpha kinase 2 interferon-induced, double-stranded RNA-activated protein kinase|P1/eIF-2A protein kinase|double stranded RNA activated protein kinase|eIF-2A protein kinase 2|interferon-inducible elF2alpha kinase|p68 kinase|protein kinase R|protein kinase, interferon-inducible double stranded RNA dependent|protein phosphatase 1, regulatory subunit 83|tyrosine-protein kinase EIF2AK2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF2AK2, check out the EIF2AK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF2AK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PKR/EIF2AK2 Antibody Picoband® (PA2025)
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No publications found for PA2025
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-PKR/EIF2AK2 Antibody Picoband®
Question
I see that the anti-PKR/EIF2AK2 antibody PA2025 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-23
Question
Does PA2025 anti-PKR/EIF2AK2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
As indicated on the product datasheet, PA2025 anti-PKR/EIF2AK2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-12
Question
See below the WB image, lot number and protocol we used for brain using anti-PKR/EIF2AK2 antibody PA2025. Please let me know if you require anything else.
C. Moore
Verified customer
Asked: 2014-04-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-04-21
