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Product Info Summary
| SKU: | P00090 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Phospho-Smad2 (S255) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00090
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Smad2 (S255) Rabbit Monoclonal Antibody catalog # P00090. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Smad2 (S255) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00090)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HBE-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Smad2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00090 is reactive to SMAD2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
100 kDa
Calculated molecular weight
52306 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00090 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
IP 1:50
Positive Control
WB: Hela cell
IHC: human transitional cell carcinoma of bladder tissue
Validation Images & Assay Conditions
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Western blot analysis of Phospho-Smad2 (S255) expression in Hela cell treated with Okadaic acid and Calyculin A lysate.
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Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder, using Phospho-Smad2 (S255) Antibody.
Protein Target Info & Infographic
Gene/Protein Information For SMAD2 (Source: Uniprot.org, NCBI)
Gene Name
SMAD2
Full Name
Mothers against decapentaplegic homolog 2
Weight
52306 MW
Superfamily
dwarfin/SMAD family
Alternative Names
Mothers against decapentaplegic homolog 2;MAD homolog 2;Mothers against DPP homolog 2;JV18-1;Mad-related protein 2;hMAD-2;SMAD family member 2;SMAD 2;Smad2;hSMAD2;SMAD2;MADH2, MADR2; SMAD2 JV18, JV18-1, MADH2, MADR2, hMAD-2, hSMAD2 SMAD family member 2 mothers against decapentaplegic homolog 2|MAD homolog 2|SMAD, mothers against DPP homolog 2|Sma- and Mad-related protein 2|mother against DPP homolog 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMAD2, check out the SMAD2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMAD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Smad2 (S255) Rabbit Monoclonal Antibody (P00090)
Hello CJ!
P00090 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Mouse hepatic neoplasm formation induced by trace level and low frequency exposure to diethylnitrosamine through %u03B2-catenin signaling pathway
Astragaloside effect on TGF-%u03B21, SMAD2/3, and %u03B1-SMA expression in the kidney tissues of diabetic KKAy mice
Effect of Kuijie Granule on the Expression of TGF-?/Smads Signaling Pathway in Patients with Ulcerative Colitis
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Phospho-Smad2 (S255) Rabbit Monoclonal Antibody
Question
We have been able to see staining in human kidney. Any tips? Is anti-Phospho-Smad2 (S255) Rabbit Monoclonal antibody supposed to stain kidney positively?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
According to literature kidney does express SMAD2. According to Uniprot.org, SMAD2 is expressed in kidney, placenta, kidney, pancreas spleen, chronic myeloid leukemia cell, colon adenocarcinoma, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have SMAD2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Chronic myeloid leukemia cell, Pubmed ID: 8980228
Colon adenocarcinoma, Pubmed ID: 9702198
Kidney, Pubmed ID: 8752209
Kidney, Pancreas, and Spleen, Pubmed ID: 15489334
Placenta, Pubmed ID: 8774881
Boster Scientific Support
Answered: 2020-04-17
Question
We are currently using anti-Phospho-Smad2 (S255) Rabbit Monoclonal antibody P00090 for human tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
The anti-Phospho-Smad2 (S255) Rabbit Monoclonal antibody (P00090) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-23
Question
Our team were happy with the WB result of your anti-Phospho-Smad2 (S255) Rabbit Monoclonal antibody. However we have been able to see positive staining in chronic myeloid leukemia cell cytoplasm using this antibody. Is that expected? Could you tell me where is SMAD2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
From what I have seen in literature, chronic myeloid leukemia cell does express SMAD2. Generally SMAD2 expresses in cytoplasm. Regarding which tissues have SMAD2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Chronic myeloid leukemia cell, Pubmed ID: 8980228
Colon adenocarcinoma, Pubmed ID: 9702198
Kidney, Pubmed ID: 8752209
Kidney, Pancreas, and Spleen, Pubmed ID: 15489334
Placenta, Pubmed ID: 8774881
Boster Scientific Support
Answered: 2019-06-21
Question
We have tried in the past anti-Phospho-Smad2 (S255) Rabbit Monoclonal antibody for IP on pancreas spleen in a previous experiment. I am using rat, and We want to use the antibody for WB next. We want examining pancreas spleen as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2017-12-15
Answer
I have checked the website and datasheets of our anti-Phospho-Smad2 (S255) Rabbit Monoclonal antibody and I see that P00090 has been validated on rat in both IP and WB. Thus P00090 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-12-15
