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Product Info Summary
| SKU: | P06527 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-Phospho RapGEF1 (Tyr504) Antibody
SKU/Catalog Number
P06527
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho RapGEF1 (Tyr504) Antibody (Catalog# P06527). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho RapGEF1 (Tyr504) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P06527)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human RapGEF1 around the phosphorylation site of Tyr504. Immunogen sequence location: 470-519
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P06527 is reactive to RAPGEF1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
120548 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P06527 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3nM 40', using RapGEF1 (Phospho-Tyr504) Antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For RAPGEF1 (Source: Uniprot.org, NCBI)
Gene Name
RAPGEF1
Full Name
Rap guanine nucleotide exchange factor 1
Weight
120548 MW
Alternative Names
RAPGEF1;GRF2;Rap guanine nucleotide exchange factor 1;CRK SH3-binding GNRP;Guanine nucleotide-releasing factor 2;Protein C3G RAPGEF1 C3G, GRF2 Rap guanine nucleotide exchange factor 1 rap guanine nucleotide exchange factor 1|CRK SH3-binding GNRP|Rap guanine nucleotide exchange factor (GEF) 1|guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAPGEF1, check out the RAPGEF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAPGEF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho RapGEF1 (Tyr504) Antibody (P06527)
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No publications found for P06527
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Customer Q&As
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1 Customer Q&As for Anti-Phospho RapGEF1 (Tyr504) Antibody
Question
We are currently using anti-Phospho RapGEF1 (Tyr504) antibody P06527 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
The anti-Phospho RapGEF1 (Tyr504) antibody (P06527) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-18
