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Product Info Summary
| SKU: | M05304 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-PDE8A Purified Monoclonal Antibody
SKU/Catalog Number
M05304
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PDE8A Purified Monoclonal Antibody (Catalog# M05304). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-PDE8A Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05304)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
EM-52
Isotype
Mouse IgG1 kappa
Immunogen
Human PDE8a. The mouse monoclonal antibody EM-52 recognizes PDE8a (phosphodiesterase 8 A), an approximately 93 kDa intracellular enzyme that hydrolyzes cAMP to 5'AMP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M05304 is reactive to PDE8A in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
93304 MW
Background of PDE8A
PDE8a (high affinity cAMP-specific and IBMX-insensitive 3´,5´-cyclic phosphodiesterase 8 A) is a cyclic nucleotide phosphodiesterase, which hydrolyzes cAMP, an important regulator and mediator of multiple cellular responses to extracellular signals. It is expressed in a wide variety of tissues including testis, ovary, small intestine, and colon. It is not expressed e.g. in thymus. PDE8a is composed of C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05304 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1-2 μg/ml.
Validation Images & Assay Conditions
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Figure 1. Western blotting validation for Anti-PDE8A Purified Monoclonal Antibody M05304
Western blotting analysis (reducing conditions) of PDE8a in HEK293T/17-PDE8a transfectants using mouse monoclonal antibody EM-52.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For PDE8A (Source: Uniprot.org, NCBI)
Gene Name
PDE8A
Full Name
High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
Weight
93304 MW
Superfamily
cyclic nucleotide phosphodiesterase family
Alternative Names
HsT19550 PDE8A HsT19550 phosphodiesterase 8A high affinity cAMP-specific and IBMX-insensitive 3,5-cyclic phosphodiesterase 8A|cAMP-specific cyclic nucleotide phosphodiesterase 8A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDE8A, check out the PDE8A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDE8A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDE8A Purified Monoclonal Antibody (M05304)
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No publications found for M05304
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