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Product Info Summary
| SKU: | A06527 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, IF (paraffin) |
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Product info
Product Name
Anti-RapGEF1/Grf2 Antibody
SKU/Catalog Number
A06527
Size
100ul
Form
Liquid
Description
Boster Bio Anti-RapGEF1/Grf2 Antibody (Catalog# A06527). Tested in IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RapGEF1/Grf2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06527)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human RAPGEF1. Immunogen sequence location: 470-519
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06527 is reactive to RAPGEF1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
120548 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06527 is guaranteed for ELISA, IHC-P, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:5000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using RapGEF1 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For RAPGEF1 (Source: Uniprot.org, NCBI)
Gene Name
RAPGEF1
Full Name
Rap guanine nucleotide exchange factor 1
Weight
120548 MW
Alternative Names
RAPGEF1;GRF2;Rap guanine nucleotide exchange factor 1;CRK SH3-binding GNRP;Guanine nucleotide-releasing factor 2;Protein C3G RAPGEF1 C3G, GRF2 Rap guanine nucleotide exchange factor 1 rap guanine nucleotide exchange factor 1|CRK SH3-binding GNRP|Rap guanine nucleotide exchange factor (GEF) 1|guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAPGEF1, check out the RAPGEF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAPGEF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RapGEF1/Grf2 Antibody (A06527)
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No publications found for A06527
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-RapGEF1/Grf2 Antibody
Question
I was wanting to use your anti-RapGEF1/Grf2 antibody for IHC for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
As indicated on the product datasheet, A06527 anti-RapGEF1/Grf2 antibody has been validated for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-17
Question
Will anti-RapGEF1/Grf2 antibody A06527 work for IHC with leukemic t-cell?
L. Zhao
Verified customer
Asked: 2019-09-26
Answer
According to the expression profile of leukemic t-cell, RAPGEF1 is highly expressed in leukemic t-cell. So, it is likely that anti-RapGEF1/Grf2 antibody A06527 will work for IHC with leukemic t-cell.
Boster Scientific Support
Answered: 2019-09-26
Question
Is this A06527 anti-RapGEF1/Grf2 antibody reactive to the isotypes of RAPGEF1?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
The immunogen of A06527 anti-RapGEF1/Grf2 antibody is A peptide derived from human RAPGEF1. Immunogen sequence location: 470-519. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-21
