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Product Info Summary
| SKU: | M07911 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-OVGP1 Antibody (N-Term)
SKU/Catalog Number
M07911
Size
50 µl
Description
Boster Bio Anti-OVGP1 Antibody (N-Term) (Catalog # M07911). Tested in WB, IHC-P, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-OVGP1 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07911)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This OVGP1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 107-137 amino acids from human OVGP1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M07911 is reactive to OVGP1 in Human
Reconstitution
Calculated molecular weight
75421 Da
Background of OVGP1
Binds to oocyte zona pellucida in vivo. May play a role in the fertilization process and/or early embryonic development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07911 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-OVGP1 Antibody (N-Term) at 1:2000 dilution
Lane 1: human placenta lysate
Lane 2: human uterus lysate
Lane 3: human ovary lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 75 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M07911 staining OVGP1 in human fallopian tube tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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Overlay histogram showing Hela cells stained with M07911 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M07911, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For OVGP1 (Source: Uniprot.org, NCBI)
Gene Name
OVGP1
Full Name
Oviduct-specific glycoprotein
Weight
75421 Da
Superfamily
glycosyl hydrolase 18 family
Alternative Names
Oviduct-specific glycoprotein, Estrogen-dependent oviduct protein, Mucin-9, Oviductal glycoprotein, Oviductin, OVGP1, MUC9, OGP OVGP1 CHIT5, EGP, MUC9, OGP oviductal glycoprotein 1 oviduct-specific glycoprotein|estrogen-dependent oviduct protein|mucin 9|oviduct glycoprotein|oviductal glycoprotein 1, 120kDa|oviductin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on OVGP1, check out the OVGP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for OVGP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-OVGP1 Antibody (N-Term) (M07911)
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