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Product Info Summary
| SKU: | P00119 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00119
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal Antibody catalog # P00119. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00119)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IBB-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-Rac1/Cdc42 (Ser71)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00119 is reactive to CDC42 in Human
Reconstitution
Observed Molecular Weight
70, 75 kDa
Calculated molecular weight
21259 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00119 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
Positive Control
WB: A431 cell
Validation Images & Assay Conditions
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Western blot analysis of Phospho-Rac1/Cdc42 (Ser71) expression in (1) A431 cell lysate treated with LP; (2) A431 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CDC42 (Source: Uniprot.org, NCBI)
Gene Name
CDC42
Full Name
Cell division control protein 42 homolog
Weight
21259 MW
Superfamily
small GTPase superfamily
Alternative Names
Cell division control protein 42 homolog;G25K GTP-binding protein;CDC42; CDC42 CDC42Hs, G25K, TKS cell division cycle 42 cell division control protein 42 homolog|G25K GTP-binding protein|GTP binding protein, 25kDa|dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD))|dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD))|growth-regulating protein|small GTP binding protein CDC42
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDC42, check out the CDC42 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDC42: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal Antibody (P00119)
Hello CJ!
No publications found for P00119
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal Antibody
Question
Do you have a BSA free version of anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody P00119 available?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody P00119 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-26
Question
We have seen staining in human placenta uterus. Are there any suggestions? Is anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody supposed to stain placenta uterus positively?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
From what I have seen in literature placenta uterus does express CDC42. From what I have seen in Uniprot.org, CDC42 is expressed in testis, fetal brain, placenta, brain placenta, cervix, placenta uterus, brain, cajal-retzius cell fetal brain cortex, neutrophil, embryo, among other tissues. Regarding which tissues have CDC42 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 10490598
Fetal brain, Pubmed ID: 2122236
Neutrophil, Pubmed ID: 8504089
Placenta, Pubmed ID: 2124704
Boster Scientific Support
Answered: 2020-03-23
Question
I was wanting to use your anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody for WB for human testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human testis identification?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
As indicated on the product datasheet, P00119 anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-11
Question
Is a blocking peptide available for product anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody (P00119)?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
We do provide the blocking peptide for product anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody (P00119). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-14
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody P00119. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-07
Question
Is this P00119 anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody reactive to the isotypes of CDC42?
J. Baker
Verified customer
Asked: 2019-07-08
Answer
The immunogen of P00119 anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody is A synthesized peptide derived from human Phospho-Rac1/Cdc42 (Ser71). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-08
Question
I have attached the WB image, lot number and protocol we used for testis using anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody P00119. Please let me know if you require anything else.
B. Johnson
Verified customer
Asked: 2019-01-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-17
Question
Our team were content with the WB result of your anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody. However we have been able to see positive staining in testis cell membrane using this antibody. Is that expected? Could you tell me where is CDC42 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-10-26
Answer
According to literature, testis does express CDC42. Generally CDC42 expresses in cell membrane. Regarding which tissues have CDC42 expression, here are a few articles citing expression in various tissues:
Embryo, Pubmed ID: 10490598
Fetal brain, Pubmed ID: 2122236
Neutrophil, Pubmed ID: 8504089
Placenta, Pubmed ID: 2124704
Boster Scientific Support
Answered: 2018-10-26
Question
We want using your anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody for regulation of attachment of spindle microtubules to kinetochore studies. Has this antibody been tested with western blotting on a431 cell lysate? We would like to see some validation images before ordering.
A. Anderson
Verified customer
Asked: 2018-06-28
Answer
We appreciate your inquiry. This P00119 anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody is tested on a431 cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-06-28
Question
Would anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody P00119 work for WB with testis?
C. Collins
Verified customer
Asked: 2017-10-16
Answer
According to the expression profile of testis, CDC42 is highly expressed in testis. So, it is likely that anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody P00119 will work for WB with testis.
Boster Scientific Support
Answered: 2017-10-16
Question
We are currently using anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody P00119 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-18
Answer
The anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody (P00119) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-18
Question
I am looking for to test anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody P00119 on human testis for research purposes, then I may be interested in using anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody P00119 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
F. Thomas
Verified customer
Asked: 2017-09-12
Answer
The products we sell, including anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody P00119, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-09-12
Question
Can you help my question with product P00119, anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Walker
Verified customer
Asked: 2017-01-09
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P00119 anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-01-09
Question
I see that the anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody P00119 works with WB, what is the protocol used to produce the result images on the product page?
K. Gonzalez
Verified customer
Asked: 2016-01-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-01-15
Question
Would P00119 anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Jha
Verified customer
Asked: 2013-12-30
Answer
It shows on the product datasheet, P00119 anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-12-30
