Anti-NECAB1 Mouse Monoclonal Antibody [Clone ID: OTI2H5]

Product Name

Anti-NECAB1 Mouse Monoclonal Antibody [Clone ID: OTI2H5]

View all NECAB1 Antibodies

SKU/Catalog Number

M13973

Size

100 µl

Description

Boster Bio NECAB1 mouse monoclonal antibody, clone OTI2H5 (formerly 2H5). Catalog# M13973. Tested in FC, WB. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C as received.

Cite This Product

Anti-NECAB1 Mouse Monoclonal Antibody [Clone ID: OTI2H5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M13973)

Host

Mouse

Contents

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Clonality

Monoclonal

Clone Number

OTI2H5

Isotype

IgG1

Immunogen

Full length human recombinant protein of human NECAB1 (NP_071746) produced in HEK293T cell.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M13973 is reactive to NECAB1 in Human, Mouse, Rat

Applications

M13973 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Validation Images & Assay Conditions

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NECAB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NECAB1.

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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NECAB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-NECAB1 monoclonal antibody (1:200).

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HEK293T cells transfected with either NECAB1 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NECAB1 antibody (M13973)

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Flow cytometric Analysis of Jurkat cells

Gene/Protein Information For NECAB1 (Source: Uniprot.org, NCBI)

Alternative Names

EF hand calcium binding protein 1; EFCBP1; EF-hand calcium binding protein 1; EF-hand calcium-binding protein 1; neuronal calcium binding protein; Neuronal calcium-binding protein 1; N-terminal EF-hand calcium binding protein 1; N-terminal EF-hand calcium-binding protein 1; STIP-1; synaptotagmin interacting protein 1 NECAB1 EFCBP1, STIP-1 N-terminal EF-hand calcium binding protein 1 N-terminal EF-hand calcium-binding protein 1|EF-hand calcium-binding protein 1|neuronal calcium binding protein|neuronal calcium-binding protein 1|synaptotagmin interacting protein 1

For more info on NECAB1, check out the NECAB1 Infographic

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In this infographic, you will see the following information for NECAB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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